Alkaline Phosphatase-Triggered Spatiotemporal Repair of Corneal Injury with TB500 Peptide Hydrogel.

Lu P, Shan M, Peng C, Ji W, Yang T, Yang Z, Zhang Z, Wang Y

ACS applied materials & interfaces8 Dec 2025

PMID41359360DOI10.1021/acsami.5c14652PepBankPUB-41359360

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study9

8 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In an alkali burn model of corneal injury, the hydrogel significantly accelerated epithelial regeneration.
In plain wordsIn an animal model of corneal burn, the TB500 hydrogel sped up healing of the surface layer.
Peptide
TB-500
Outcome
IncreaseEpithelial regeneration
Study
n: not reported in source
alkali burn model of corneal injury
Outcome group
Wound healing · Eye
Studied dose
Dose not stated
Source sentences
Summary
In an alkali burn model, the hydrogel significantly accelerated epithelial regeneration, reduced inflammation, and improved corneal barrier function.
1 context sentence(design, population, supporting numbers)
Summary · context
The hydrogel is designed through enzyme-instructed self-assembly (EISA) of a phosphorylated peptide precursor, Nap-YpYY-TB500, which undergoes site-specific dephosphorylation by elevated ALP levels at the wound site, triggering nanofiber formation and gelation in situ.
Report a problemFND-4135936006
02AnimalAnimal study (in vivo)Efficacy outcome
In an alkali burn model of corneal injury, the hydrogel reduced inflammation.
In plain wordsIn an animal model of corneal burn, the TB500 hydrogel reduced inflammation.
Peptide
TB-500
Outcome
DecreaseCorneal inflammation
Study
n: not reported in source
alkali burn model of corneal injury
Outcome group
Inflammation markers · Eye
Studied dose
Dose not stated
Source sentences
Summary
In an alkali burn model, the hydrogel significantly accelerated epithelial regeneration, reduced inflammation, and improved corneal barrier function.
1 context sentence(design, population, supporting numbers)
Summary · context
The hydrogel is designed through enzyme-instructed self-assembly (EISA) of a phosphorylated peptide precursor, Nap-YpYY-TB500, which undergoes site-specific dephosphorylation by elevated ALP levels at the wound site, triggering nanofiber formation and gelation in situ.
Report a problemFND-4135936007
03AnimalAnimal study (in vivo)Efficacy outcome
In an alkali burn model of corneal injury, the hydrogel improved corneal barrier function.
In plain wordsIn an animal model of corneal burn, the TB500 hydrogel improved how well the cornea acts as a barrier.
Peptide
TB-500
Outcome
IncreaseCorneal barrier function
Study
n: not reported in source
alkali burn model of corneal injury
Outcome group
Wound healing · Eye
Studied dose
Dose not stated
Source sentences
Summary
In an alkali burn model, the hydrogel significantly accelerated epithelial regeneration, reduced inflammation, and improved corneal barrier function.
1 context sentence(design, population, supporting numbers)
Summary · context
The hydrogel is designed through enzyme-instructed self-assembly (EISA) of a phosphorylated peptide precursor, Nap-YpYY-TB500, which undergoes site-specific dephosphorylation by elevated ALP levels at the wound site, triggering nanofiber formation and gelation in situ.
Report a problemFND-4135936008
04LabIn vitro (cells)Efficacy outcome
In human corneal epithelial cells, the Nap-YpYY-TB500 hydrogel promoted cell migration.
In plain wordsIn lab-grown human corneal cells, the TB500 hydrogel helped the cells move.
Peptide
TB-500
Outcome
IncreaseHCEC migration
Study
humann: not reported in source
human corneal epithelial cells (HCEC)
Outcome group
Wound healing · Eye
Studied dose
Dose not stated
Source sentences
Summary
In vitro, the hydrogel promoted human corneal epithelial cell (HCEC) migration, proliferation, and tight junction recovery, while also enhancing myofibroblastic differentiation and cytoskeletal reorganization of human corneal stromal fibroblasts (HCSFs).
1 context sentence(design, population, supporting numbers)
Summary · context
The hydrogel is designed through enzyme-instructed self-assembly (EISA) of a phosphorylated peptide precursor, Nap-YpYY-TB500, which undergoes site-specific dephosphorylation by elevated ALP levels at the wound site, triggering nanofiber formation and gelation in situ.
Report a problemFND-4135936001
05LabIn vitro (cells)Efficacy outcome
In human corneal epithelial cells, the Nap-YpYY-TB500 hydrogel promoted cell proliferation.
In plain wordsIn lab-grown human corneal cells, the TB500 hydrogel helped the cells multiply.
Peptide
TB-500
Outcome
IncreaseHCEC proliferation
Study
humann: not reported in source
human corneal epithelial cells (HCEC)
Outcome group
Wound healing · Eye
Studied dose
Dose not stated
Source sentences
Summary
In vitro, the hydrogel promoted human corneal epithelial cell (HCEC) migration, proliferation, and tight junction recovery, while also enhancing myofibroblastic differentiation and cytoskeletal reorganization of human corneal stromal fibroblasts (HCSFs).
1 context sentence(design, population, supporting numbers)
Summary · context
The hydrogel is designed through enzyme-instructed self-assembly (EISA) of a phosphorylated peptide precursor, Nap-YpYY-TB500, which undergoes site-specific dephosphorylation by elevated ALP levels at the wound site, triggering nanofiber formation and gelation in situ.
Report a problemFND-4135936002
06LabIn vitro (cells)Efficacy outcome
In human corneal epithelial cells, the Nap-YpYY-TB500 hydrogel promoted tight junction recovery.
In plain wordsIn lab-grown human corneal cells, the TB500 hydrogel helped cell-to-cell seals recover.
Peptide
TB-500
Outcome
IncreaseHCEC tight junction recovery
Study
humann: not reported in source
human corneal epithelial cells (HCEC)
Outcome group
Wound healing · Eye
Studied dose
Dose not stated
Source sentences
Summary
In vitro, the hydrogel promoted human corneal epithelial cell (HCEC) migration, proliferation, and tight junction recovery, while also enhancing myofibroblastic differentiation and cytoskeletal reorganization of human corneal stromal fibroblasts (HCSFs).
1 context sentence(design, population, supporting numbers)
Summary · context
The hydrogel is designed through enzyme-instructed self-assembly (EISA) of a phosphorylated peptide precursor, Nap-YpYY-TB500, which undergoes site-specific dephosphorylation by elevated ALP levels at the wound site, triggering nanofiber formation and gelation in situ.
Report a problemFND-4135936003
07LabIn vitro (cells)Efficacy outcome
In human corneal stromal fibroblasts, the Nap-YpYY-TB500 hydrogel enhanced myofibroblastic differentiation.
In plain wordsIn lab-grown human corneal cells, the TB500 hydrogel increased the change of fibroblasts into repair-type cells.
Peptide
TB-500
Outcome
IncreaseHCSF myofibroblastic differentiation
Study
humann: not reported in source
human corneal stromal fibroblasts (HCSF)
Outcome group
Wound healing · Eye
Studied dose
Dose not stated
Source sentences
Summary
In vitro, the hydrogel promoted human corneal epithelial cell (HCEC) migration, proliferation, and tight junction recovery, while also enhancing myofibroblastic differentiation and cytoskeletal reorganization of human corneal stromal fibroblasts (HCSFs).
1 context sentence(design, population, supporting numbers)
Summary · context
The hydrogel is designed through enzyme-instructed self-assembly (EISA) of a phosphorylated peptide precursor, Nap-YpYY-TB500, which undergoes site-specific dephosphorylation by elevated ALP levels at the wound site, triggering nanofiber formation and gelation in situ.
Report a problemFND-4135936004
08LabIn vitro (cells)Efficacy outcome
In human corneal stromal fibroblasts, the Nap-YpYY-TB500 hydrogel enhanced cytoskeletal reorganization.
In plain wordsIn lab-grown human corneal cells, the TB500 hydrogel increased the rebuilding of the cell skeleton.
Peptide
TB-500
Outcome
IncreaseHCSF cytoskeletal reorganization
Study
humann: not reported in source
human corneal stromal fibroblasts (HCSF)
Outcome group
Wound healing · Eye
Studied dose
Dose not stated
Source sentences
Summary
In vitro, the hydrogel promoted human corneal epithelial cell (HCEC) migration, proliferation, and tight junction recovery, while also enhancing myofibroblastic differentiation and cytoskeletal reorganization of human corneal stromal fibroblasts (HCSFs).
1 context sentence(design, population, supporting numbers)
Summary · context
The hydrogel is designed through enzyme-instructed self-assembly (EISA) of a phosphorylated peptide precursor, Nap-YpYY-TB500, which undergoes site-specific dephosphorylation by elevated ALP levels at the wound site, triggering nanofiber formation and gelation in situ.
Report a problemFND-4135936005

Studies that did not test the peptide alone1

In this study, TB-500 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking TB-500.

09LabNot a test of TB-500 aloneIn vitro (cell-free)Other
Among three candidate sequences, Nap-YpYY-TB500 showed optimal gelation kinetics, nanostructure and therapeutic efficacy.
In plain wordsIn lab tests, the Nap-YpYY-TB500 version gelled best and had the best structure and effect among three candidates.
Peptide
TB-500
Outcome
Not applicableGelation kinetics, nanostructure and efficacy of candidate sequences
Study
n: not reported in source
Outcome group
Formulation, delivery and stability · Pharmacology
Source sentences
Summary
Among three candidate sequences, Nap-YpYY-TB500 exhibited optimal gelation kinetics, nanostructure, and therapeutic efficacy.
1 context sentence(design, population, supporting numbers)
Summary · context
The hydrogel is designed through enzyme-instructed self-assembly (EISA) of a phosphorylated peptide precursor, Nap-YpYY-TB500, which undergoes site-specific dephosphorylation by elevated ALP levels at the wound site, triggering nanofiber formation and gelation in situ.
In this study, TB-500 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking TB-500.
Report a problemFND-4135936009

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 3Current
9 Oct 2026, 18:17 UTC
Study results updated
Version 2
9 Oct 2026, 03:03 UTC
Study data updated · source update from Europe PMC
Version 1
9 Oct 2026, 03:02 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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Keywords

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