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Study · Animal study (in vivo) · rat · 2023

8 results

Effect of ACTH4-10Pro8-Gly9-Pro10 on anti-inflammatory cytokine (IL-4, IL-10, IL-13) expression in acute spinal cord injury models (male Sprague Dawley rats).

Asadullah A, Bajamal AH, Parenrengi MA, Turchan A, Utomo B, Sudiana IK, Subagio EA

F1000Research1 Jan 2023

PMID41179234 (opens in a new tab)PMCIDPMC12579320 (opens in a new tab)DOI10.12688/f1000research.127413.2 (opens in a new tab)PepBankPUB-41179234
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Summary

BackgroundSpinal cord injury (SCI) is a damage to the spinal cord caused mainly by trauma resulting in major motor, sensory and autonomic dysfunctions. Its final neurological outcome is determined by both primary and secondary injury processes. A key component of secondary injury mechanisms after initial trauma is neuroinflammation. A neuroprotective compound, ACTH 4-10Pro 8-Gly 9-Pro 10 (ACTH 4-10) also known as semax, has shown neuroprotective and anti-inflammatory properties. ACTH 4-10 has also been actively used in the treatment of brain ischemia without serious complication reported. Here, we analyzed the effects of ACTH 4-10 at regulating the inflammatory cascade in SCI by looking at anti-inflammatory cytokine (IL-4, IL-10 and IL-13) levels after acute SCI.MethodWe carried out laminectomies in male Sprague Dawley rats at the second thoracic vertebrae. After laminectomy, we exposed the myelum and created mild SCI models with 20-g, and severe SCI with 35-g aneurysm clips. ACTH 4-10 was administered intranasally to the treatment group and 0.9% NaCl to the control group (placebo). Both groups were kept alive and terminated at 3 and 6 hours. The tissue sample preparations were fixed in formalin and examined for immunohistochemistry. Quantitative measurement of the cytokines was done in the posterior horn area with specific associated anti-monoclonal antibodies.ResultsRats with mild SCI that were given ACTH 4-10 showed greater anti-inflammatory levels at 3 hours post-compression but only IL-10 and IL-13 were elevated significantly at 6 hours. Rats with severe compression in ACTH 4-10 group showed greater levels of IL-10, IL-13 at 3 hours and IL-4, IL-10 at 6 hours compared with the placebo group.ConclusionsAdministration of ACTH 4-10Pro 8-Gly 9-Pro 10 intranasal can increase anti-inflammatory cytokine expression in Sprague Dawley rat models with mild and severe SCI. Expression of anti-inflammatory cytokines was greater in mild compression and 3-hour termination. Further research is needed to determine the optimal dose and clinical outcome in vivo.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study8

8 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In rats with mild spinal cord injury, intranasal ACTH 4-10 (Semax) gave greater anti-inflammatory cytokine levels than placebo at 3 hours.

    In plain wordsIn rats with mild spinal cord injury, Semax nose treatment gave higher levels of anti-inflammatory signals than placebo after 3 hours.

    Peptide
    Semax
    Outcome
    IncreaseAnti-inflammatory cytokine levels at 3 h, mild SCI
    Study

    ratn: not reported in source

    acute spinal cord injury (SCI) model
    Comparator
    Placebo (0.9% NaCl)
    Outcome group
    Inflammation markers · Immune and inflammation
    Studied dose
    Dose not stated · intranasalAs given in this study (rat).

    Source sentences

    Summary
    Rats with mild SCI that were given ACTH 4-10 showed greater anti-inflammatory levels at 3 hours post-compression but only IL-10 and IL-13 were elevated significantly at 6 hours.
    1 context sentence
    Summary · context
    After laminectomy, we exposed the myelum and created mild SCI models with 20-g, and severe SCI with 35-g aneurysm clips. ACTH 4-10 was administered intranasally to the treatment group and 0.9% NaCl to the control group (placebo).
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In rats with mild spinal cord injury, intranasal ACTH 4-10 (Semax) significantly raised IL-10 at 6 hours.

    In plain wordsIn rats with mild spinal cord injury, Semax nose treatment raised the anti-inflammatory signal IL-10 after 6 hours.

    Peptide
    Semax
    Outcome
    IncreaseIL-10 at 6 h, mild SCI
    Study

    ratn: not reported in source

    acute spinal cord injury (SCI) model
    Comparator
    Placebo (0.9% NaCl)
    Outcome group
    Inflammation markers · Immune and inflammation
    Studied dose
    Dose not stated · intranasalAs given in this study (rat).

    Source sentences

    Summary
    Rats with mild SCI that were given ACTH 4-10 showed greater anti-inflammatory levels at 3 hours post-compression but only IL-10 and IL-13 were elevated significantly at 6 hours.
    1 context sentence
    Summary · context
    After laminectomy, we exposed the myelum and created mild SCI models with 20-g, and severe SCI with 35-g aneurysm clips. ACTH 4-10 was administered intranasally to the treatment group and 0.9% NaCl to the control group (placebo).
  3. 03AnimalAnimal study (in vivo)Efficacy outcome

    In rats with mild spinal cord injury, intranasal ACTH 4-10 (Semax) significantly raised IL-13 at 6 hours.

    In plain wordsIn rats with mild spinal cord injury, Semax nose treatment raised the anti-inflammatory signal IL-13 after 6 hours.

    Peptide
    Semax
    Outcome
    IncreaseIL-13 at 6 h, mild SCI
    Study

    ratn: not reported in source

    acute spinal cord injury (SCI) model
    Comparator
    Placebo (0.9% NaCl)
    Outcome group
    Inflammation markers · Immune and inflammation
    Studied dose
    Dose not stated · intranasalAs given in this study (rat).

    Source sentences

    Summary
    Rats with mild SCI that were given ACTH 4-10 showed greater anti-inflammatory levels at 3 hours post-compression but only IL-10 and IL-13 were elevated significantly at 6 hours.
    1 context sentence
    Summary · context
    After laminectomy, we exposed the myelum and created mild SCI models with 20-g, and severe SCI with 35-g aneurysm clips. ACTH 4-10 was administered intranasally to the treatment group and 0.9% NaCl to the control group (placebo).
  4. 04AnimalAnimal study (in vivo)Efficacy outcome

    In rats with mild spinal cord injury, IL-4 was not significantly elevated at 6 hours after intranasal ACTH 4-10 (Semax); only IL-10 and IL-13 were.

    In plain wordsIn rats with mild spinal cord injury, Semax nose treatment did not significantly raise IL-4 after 6 hours.

    Peptide
    Semax
    Outcome
    No significant changeIL-4 at 6 h, mild SCI
    Study

    ratn: not reported in source

    acute spinal cord injury (SCI) model
    Comparator
    Placebo (0.9% NaCl)
    Outcome group
    Inflammation markers · Immune and inflammation
    Studied dose
    Dose not stated · intranasalAs given in this study (rat).

    Source sentences

    Summary
    Rats with mild SCI that were given ACTH 4-10 showed greater anti-inflammatory levels at 3 hours post-compression but only IL-10 and IL-13 were elevated significantly at 6 hours.
    1 context sentence
    Summary · context
    After laminectomy, we exposed the myelum and created mild SCI models with 20-g, and severe SCI with 35-g aneurysm clips. ACTH 4-10 was administered intranasally to the treatment group and 0.9% NaCl to the control group (placebo).
  5. 05AnimalAnimal study (in vivo)Efficacy outcome

    In rats with severe spinal cord injury, intranasal ACTH 4-10 (Semax) gave greater IL-10 than placebo at 3 hours.

    In plain wordsIn rats with severe spinal cord injury, Semax nose treatment gave higher IL-10 than placebo after 3 hours.

    Peptide
    Semax
    Outcome
    IncreaseIL-10 at 3 h, severe SCI
    Study

    ratn: not reported in source

    acute spinal cord injury (SCI) model
    Comparator
    Placebo (0.9% NaCl)
    Outcome group
    Inflammation markers · Immune and inflammation
    Studied dose
    Dose not stated · intranasalAs given in this study (rat).

    Source sentences

    Summary
    Rats with severe compression in ACTH 4-10 group showed greater levels of IL-10, IL-13 at 3 hours and IL-4, IL-10 at 6 hours compared with the placebo group.
    1 context sentence
    Summary · context
    After laminectomy, we exposed the myelum and created mild SCI models with 20-g, and severe SCI with 35-g aneurysm clips. ACTH 4-10 was administered intranasally to the treatment group and 0.9% NaCl to the control group (placebo).
  6. 06AnimalAnimal study (in vivo)Efficacy outcome

    In rats with severe spinal cord injury, intranasal ACTH 4-10 (Semax) gave greater IL-13 than placebo at 3 hours.

    In plain wordsIn rats with severe spinal cord injury, Semax nose treatment gave higher IL-13 than placebo after 3 hours.

    Peptide
    Semax
    Outcome
    IncreaseIL-13 at 3 h, severe SCI
    Study

    ratn: not reported in source

    acute spinal cord injury (SCI) model
    Comparator
    Placebo (0.9% NaCl)
    Outcome group
    Inflammation markers · Immune and inflammation
    Studied dose
    Dose not stated · intranasalAs given in this study (rat).

    Source sentences

    Summary
    Rats with severe compression in ACTH 4-10 group showed greater levels of IL-10, IL-13 at 3 hours and IL-4, IL-10 at 6 hours compared with the placebo group.
    1 context sentence
    Summary · context
    After laminectomy, we exposed the myelum and created mild SCI models with 20-g, and severe SCI with 35-g aneurysm clips. ACTH 4-10 was administered intranasally to the treatment group and 0.9% NaCl to the control group (placebo).
  7. 07AnimalAnimal study (in vivo)Efficacy outcome

    In rats with severe spinal cord injury, intranasal ACTH 4-10 (Semax) gave greater IL-4 than placebo at 6 hours.

    In plain wordsIn rats with severe spinal cord injury, Semax nose treatment gave higher IL-4 than placebo after 6 hours.

    Peptide
    Semax
    Outcome
    IncreaseIL-4 at 6 h, severe SCI
    Study

    ratn: not reported in source

    acute spinal cord injury (SCI) model
    Comparator
    Placebo (0.9% NaCl)
    Outcome group
    Inflammation markers · Immune and inflammation
    Studied dose
    Dose not stated · intranasalAs given in this study (rat).

    Source sentences

    Summary
    Rats with severe compression in ACTH 4-10 group showed greater levels of IL-10, IL-13 at 3 hours and IL-4, IL-10 at 6 hours compared with the placebo group.
    1 context sentence
    Summary · context
    After laminectomy, we exposed the myelum and created mild SCI models with 20-g, and severe SCI with 35-g aneurysm clips. ACTH 4-10 was administered intranasally to the treatment group and 0.9% NaCl to the control group (placebo).
  8. 08AnimalAnimal study (in vivo)Efficacy outcome

    In rats with severe spinal cord injury, intranasal ACTH 4-10 (Semax) gave greater IL-10 than placebo at 6 hours.

    In plain wordsIn rats with severe spinal cord injury, Semax nose treatment gave higher IL-10 than placebo after 6 hours.

    Peptide
    Semax
    Outcome
    IncreaseIL-10 at 6 h, severe SCI
    Study

    ratn: not reported in source

    acute spinal cord injury (SCI) model
    Comparator
    Placebo (0.9% NaCl)
    Outcome group
    Inflammation markers · Immune and inflammation
    Studied dose
    Dose not stated · intranasalAs given in this study (rat).

    Source sentences

    Summary
    Rats with severe compression in ACTH 4-10 group showed greater levels of IL-10, IL-13 at 3 hours and IL-4, IL-10 at 6 hours compared with the placebo group.
    1 context sentence
    Summary · context
    After laminectomy, we exposed the myelum and created mild SCI models with 20-g, and severe SCI with 35-g aneurysm clips. ACTH 4-10 was administered intranasally to the treatment group and 0.9% NaCl to the control group (placebo).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    9 Oct 2026, 19:08 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

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