Assessing The Effectiveness of Growth Hormone Releasing Protein-6 in Improving Human Oocyte Maturation and Meiotic Progression in In Vitro Maturation Culture Media.

Ostadian C, Hayati N, Zahiri Sorouri Z, Hosseini A

International journal of fertility & sterility30 Sept 2025Cited by 1 (Europe PMC)

PMID41090425PMCIDPMC12530215DOI10.22074/ijfs.2025.2038272.1745PepBankPUB-41090425

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: CC BY-NC. Results still quote short attributed sentences.

Results from this study7

7 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueEfficacy outcome
In cultured human oocytes, GHRP‑6 yielded 70% maturation on the first day, exceeding blastocyst medium.
In plain wordsIn lab tests, human oocyte maturation reached 70% with GHRP‑6 on the first day, exceeding blastocyst medium.
Peptide
GHRP-6
Outcome
IncreaseFirst-day maturation rate versus blastocyst medium
Study
humann: not reported in source
Human germinal vesicle oocytes in maturation culture
Comparator
Blastocyst medium
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
75 ng/ml · culture medium · 1 dayAs given in this study (human).
Measurements
Maturation rate
70%
first day; GHRP-6 75 ng/ml
Source sentences
Summary
The optimal concentration of GHRP-6 for the IVM procedure was determined to be 75 ng/ml, resulting in a maturation rate of 70% on the first day and 80% on the second day.
3 context sentences(design, population, supporting numbers)
Summary · context
These results surpassed those of other culture media on both days.
Summary · context
In this experimental study, a total of 240 human germinal vesicle (GV) oocytes were collected and cultured in varying concentrations of GHRP-6.
Summary · context
Maturation rates were assessed during two days of culture, and compared against a blastocyst media (single-step culture) as control group and another IVM media, human tubal fluid (HTF) 10%, as the sham group.
Report a problemFND-4109042501
02LabEx vivo tissueEfficacy outcome
In cultured human oocytes, GHRP‑6 yielded 70% maturation on the first day, exceeding HTF medium.
In plain wordsIn lab tests, human oocyte maturation reached 70% with GHRP‑6 on the first day, exceeding HTF medium.
Peptide
GHRP-6
Outcome
IncreaseFirst-day maturation rate versus HTF medium
Study
humann: not reported in source
Human germinal vesicle oocytes in maturation culture
Comparator
HTF 10% medium
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
75 ng/ml · culture medium · 1 dayAs given in this study (human).
Measurements
Maturation rate
70%
first day; GHRP-6 75 ng/ml
Source sentences
Summary
The optimal concentration of GHRP-6 for the IVM procedure was determined to be 75 ng/ml, resulting in a maturation rate of 70% on the first day and 80% on the second day.
3 context sentences(design, population, supporting numbers)
Summary · context
These results surpassed those of other culture media on both days.
Summary · context
In this experimental study, a total of 240 human germinal vesicle (GV) oocytes were collected and cultured in varying concentrations of GHRP-6.
Summary · context
Maturation rates were assessed during two days of culture, and compared against a blastocyst media (single-step culture) as control group and another IVM media, human tubal fluid (HTF) 10%, as the sham group.
Report a problemFND-4109042502
03LabEx vivo tissueEfficacy outcome
In cultured human oocytes, GHRP‑6 yielded 80% maturation on the second day, exceeding blastocyst medium.
In plain wordsIn lab tests, human oocyte maturation reached 80% with GHRP‑6 on the second day, exceeding blastocyst medium.
Peptide
GHRP-6
Outcome
IncreaseSecond-day maturation rate versus blastocyst medium
Study
humann: not reported in source
Human germinal vesicle oocytes in maturation culture
Comparator
Blastocyst medium
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
75 ng/ml · culture medium · 2 daysAs given in this study (human).
Measurements
Maturation rate
80%
second day; GHRP-6 75 ng/ml
Source sentences
Summary
The optimal concentration of GHRP-6 for the IVM procedure was determined to be 75 ng/ml, resulting in a maturation rate of 70% on the first day and 80% on the second day.
3 context sentences(design, population, supporting numbers)
Summary · context
These results surpassed those of other culture media on both days.
Summary · context
In this experimental study, a total of 240 human germinal vesicle (GV) oocytes were collected and cultured in varying concentrations of GHRP-6.
Summary · context
Maturation rates were assessed during two days of culture, and compared against a blastocyst media (single-step culture) as control group and another IVM media, human tubal fluid (HTF) 10%, as the sham group.
Report a problemFND-4109042503
04LabEx vivo tissueEfficacy outcome
In cultured human oocytes, GHRP‑6 yielded 80% maturation on the second day, exceeding HTF medium.
In plain wordsIn lab tests, human oocyte maturation reached 80% with GHRP‑6 on the second day, exceeding HTF medium.
Peptide
GHRP-6
Outcome
IncreaseSecond-day maturation rate versus HTF medium
Study
humann: not reported in source
Human germinal vesicle oocytes in maturation culture
Comparator
HTF 10% medium
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
75 ng/ml · culture medium · 2 daysAs given in this study (human).
Measurements
Maturation rate
80%
second day; GHRP-6 75 ng/ml
Source sentences
Summary
The optimal concentration of GHRP-6 for the IVM procedure was determined to be 75 ng/ml, resulting in a maturation rate of 70% on the first day and 80% on the second day.
3 context sentences(design, population, supporting numbers)
Summary · context
These results surpassed those of other culture media on both days.
Summary · context
In this experimental study, a total of 240 human germinal vesicle (GV) oocytes were collected and cultured in varying concentrations of GHRP-6.
Summary · context
Maturation rates were assessed during two days of culture, and compared against a blastocyst media (single-step culture) as control group and another IVM media, human tubal fluid (HTF) 10%, as the sham group.
Report a problemFND-4109042504
05LabEx vivo tissueMechanism
In cultured human oocytes, GHRP‑6 did not elevate CENP-E expression despite early first polar body appearance.
In plain wordsIn lab tests, GHRP‑6 did not increase CENP-E gene expression in human oocytes.
Peptide
GHRP-6
Outcome
No significant changeCENP-E expression
Study
humann: not reported in source
Human germinal vesicle oocytes in maturation culture
Comparator
Control and sham culture groups
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
75 ng/ml · culture medium · 24 hoursAs given in this study (human).
Source sentences
Summary
Real-time PCR data indicated that, despite the early appearance of the first polar body (PB1) on 24h of culture, the Ghrelin agonist did not elevate the expression levels of CENP-E or LINGO2, genes associated with meiotic progression and membrane proteins, respectively.
3 context sentences(design, population, supporting numbers)
Summary · context
Additionally, the expression levels of two genes associated with nuclear and cytoplasmic maturation were compared in the number of 164 GV oocytes randomly cultured in the most effective concentration of GHRP-6, control and sham groups for 24 hours, using real-time polymerase chain reaction (PCR).
Summary · context
In this experimental study, a total of 240 human germinal vesicle (GV) oocytes were collected and cultured in varying concentrations of GHRP-6.
Summary · context
Maturation rates were assessed during two days of culture, and compared against a blastocyst media (single-step culture) as control group and another IVM media, human tubal fluid (HTF) 10%, as the sham group.
Report a problemFND-4109042505
06LabEx vivo tissueMechanism
In cultured human oocytes, GHRP‑6 did not elevate LINGO2 expression despite early first polar body appearance.
In plain wordsIn lab tests, GHRP‑6 did not increase LINGO2 gene expression in human oocytes.
Peptide
GHRP-6
Outcome
No significant changeLINGO2 expression
Study
humann: not reported in source
Human germinal vesicle oocytes in maturation culture
Comparator
Control and sham culture groups
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
75 ng/ml · culture medium · 24 hoursAs given in this study (human).
Source sentences
Summary
Real-time PCR data indicated that, despite the early appearance of the first polar body (PB1) on 24h of culture, the Ghrelin agonist did not elevate the expression levels of CENP-E or LINGO2, genes associated with meiotic progression and membrane proteins, respectively.
3 context sentences(design, population, supporting numbers)
Summary · context
Additionally, the expression levels of two genes associated with nuclear and cytoplasmic maturation were compared in the number of 164 GV oocytes randomly cultured in the most effective concentration of GHRP-6, control and sham groups for 24 hours, using real-time polymerase chain reaction (PCR).
Summary · context
In this experimental study, a total of 240 human germinal vesicle (GV) oocytes were collected and cultured in varying concentrations of GHRP-6.
Summary · context
Maturation rates were assessed during two days of culture, and compared against a blastocyst media (single-step culture) as control group and another IVM media, human tubal fluid (HTF) 10%, as the sham group.
Report a problemFND-4109042506
07LabEx vivo tissueEfficacy outcome
In cultured human oocytes receiving GHRP‑6, the first polar body appeared early during culture.
In plain wordsIn lab tests, human oocytes receiving GHRP‑6 showed early first polar body appearance.
Peptide
GHRP-6
Outcome
Not applicableEarly first polar body appearance
Study
humann: not reported in source
Human germinal vesicle oocytes in maturation culture
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
75 ng/ml · culture medium · 24 hoursAs given in this study (human).
Source sentences
Summary
Real-time PCR data indicated that, despite the early appearance of the first polar body (PB1) on 24h of culture, the Ghrelin agonist did not elevate the expression levels of CENP-E or LINGO2, genes associated with meiotic progression and membrane proteins, respectively.
3 context sentences(design, population, supporting numbers)
Summary · context
Additionally, the expression levels of two genes associated with nuclear and cytoplasmic maturation were compared in the number of 164 GV oocytes randomly cultured in the most effective concentration of GHRP-6, control and sham groups for 24 hours, using real-time polymerase chain reaction (PCR).
Summary · context
In this experimental study, a total of 240 human germinal vesicle (GV) oocytes were collected and cultured in varying concentrations of GHRP-6.
Summary · context
Maturation rates were assessed during two days of culture, and compared against a blastocyst media (single-step culture) as control group and another IVM media, human tubal fluid (HTF) 10%, as the sham group.
Report a problemFND-4109042507

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
9 Oct 2026, 03:13 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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Keywords

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