The impact of mitokine MOTS-c administration on the soleus muscle of rats subjected to a 7-day hindlimb suspension.

Sidorenko DA, Lvova ID, Tyganov SA, Shenkman BS, Sharlo KA

Journal of muscle research and cell motility3 Jul 2025Cited by 2 (Europe PMC)

PMID40608240DOI10.1007/s10974-025-09700-3PepBankPUB-40608240

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study10

10 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In rats during 7-day hindlimb unloading, daily MOTS‑c prevented the increase in soleus muscle fatigue seen in the ex vivo fatigue test.
In plain wordsIn rats, MOTS‑c injections prevented increased muscle fatigue during 7 days of hindlimb unloading.
Peptide
MOTS-c
Outcome
DecreaseSoleus muscle fatigue (ex vivo test)
Study
ratn: not reported in source
7-day hindlimb unloading
Comparator
Hindlimb unloading group
Outcome group
Exercise and physical function · Musculoskeletal
Studied dose
Dose not stated · intraperitoneal · 7 daysAs given in this study (rat).
Source sentences
Summary
After the end of the experiment, we conducted an ex vivo fatigue test of soleus muscle and showed that the MOTS-c administration prevents increased fatigue during 7-day hind limb unloading.
1 context sentence(design, population, supporting numbers)
Summary · context
We daily intraperitoneally injected male Wistar rats with a short mitochondrial peptide MOTS-c during 7-day unloading of their hind limbs.
Report a problemFND-4060824001
02AnimalAnimal study (in vivo)Efficacy outcome
In rats during 7-day hindlimb unloading, MOTS‑c prevented the transformation of slow fibers into fast ones in the soleus muscle.
In plain wordsIn rats, MOTS‑c prevented slow muscle fibers from turning into fast fibers during 7 days of hindlimb unloading.
Peptide
MOTS-c
Outcome
DecreaseSlow-to-fast fiber transformation in soleus
Study
ratn: not reported in source
7-day hindlimb unloading
Comparator
Hindlimb unloading group
Outcome group
Muscle injury and repair · Musculoskeletal
Studied dose
Dose not stated · intraperitoneal · 7 daysAs given in this study (rat).
Source sentences
Summary
Also, using immunohistochemical analysis, we showed that MOTS-c prevents the transformation of slow fibers into fast ones
1 context sentence(design, population, supporting numbers)
Summary · context
We daily intraperitoneally injected male Wistar rats with a short mitochondrial peptide MOTS-c during 7-day unloading of their hind limbs.
Report a problemFND-4060824002
03AnimalAnimal study (in vivo)Efficacy outcome
In rats during 7-day hindlimb unloading, MOTS‑c mitigated atrophy of slow fibers of the soleus muscle.
In plain wordsIn rats, MOTS‑c reduced the shrinking of slow muscle fibers during 7 days of hindlimb unloading.
Peptide
MOTS-c
Outcome
DecreaseAtrophy of slow soleus fibers
Study
ratn: not reported in source
7-day hindlimb unloading
Comparator
Hindlimb unloading group
Outcome group
Muscle injury and repair · Musculoskeletal
Studied dose
Dose not stated · intraperitoneal · 7 daysAs given in this study (rat).
Source sentences
Summary
mitigates the slow muscle atrophy fibers (but not fast ones)
1 context sentence(design, population, supporting numbers)
Summary · context
We daily intraperitoneally injected male Wistar rats with a short mitochondrial peptide MOTS-c during 7-day unloading of their hind limbs.
Report a problemFND-4060824003
04AnimalAnimal study (in vivo)Efficacy outcome
In rats during 7-day hindlimb unloading, MOTS‑c did not mitigate atrophy of fast fibers of the soleus muscle.
In plain wordsIn rats, MOTS‑c did not reduce the shrinking of fast muscle fibers during 7 days of hindlimb unloading.
Peptide
MOTS-c
Outcome
No significant changeAtrophy of fast soleus fibers
Study
ratn: not reported in source
7-day hindlimb unloading
Comparator
Hindlimb unloading group
Outcome group
Muscle injury and repair · Musculoskeletal
Studied dose
Dose not stated · intraperitoneal · 7 daysAs given in this study (rat).
Source sentences
Summary
mitigates the slow muscle atrophy fibers (but not fast ones)
1 context sentence(design, population, supporting numbers)
Summary · context
We daily intraperitoneally injected male Wistar rats with a short mitochondrial peptide MOTS-c during 7-day unloading of their hind limbs.
Report a problemFND-4060824004
05AnimalAnimal study (in vivo)Mechanism
In rats during 7-day hindlimb unloading, MOTS‑c prevented the decrease in Akt and GSK3β phosphorylation in the soleus muscle.
In plain wordsIn rats, MOTS‑c prevented the drop in two muscle signalling markers (Akt and GSK3β phosphorylation) during hindlimb unloading.
Peptide
MOTS-c
Outcome
IncreaseAkt and GSK3β phosphorylation in soleus
Study
ratn: not reported in source
7-day hindlimb unloading
Comparator
Hindlimb unloading group
Outcome group
Muscle injury and repair · Musculoskeletal
Studied dose
Dose not stated · intraperitoneal · 7 daysAs given in this study (rat).
Source sentences
Summary
In the group receiving MOTS-c, the decrease in Akt and GSK3β phosphorylation was prevented
1 context sentence(design, population, supporting numbers)
Summary · context
We daily intraperitoneally injected male Wistar rats with a short mitochondrial peptide MOTS-c during 7-day unloading of their hind limbs.
Report a problemFND-4060824005
06AnimalAnimal study (in vivo)Mechanism
In rats during 7-day hindlimb unloading, 18 S and 28 S rRNA levels in MOTS‑c treated animals stayed at the control level.
In plain wordsIn rats given MOTS‑c during hindlimb unloading, muscle ribosomal RNA levels stayed at the control level.
Peptide
MOTS-c
Outcome
No significant change18 S and 28 S rRNA levels in soleus
Study
ratn: not reported in source
7-day hindlimb unloading
Comparator
Hindlimb unloading group
Outcome group
Muscle injury and repair · Musculoskeletal
Studied dose
Dose not stated · intraperitoneal · 7 daysAs given in this study (rat).
Source sentences
Summary
and the 18 S and 28 S rRNA levels were at the control level.
1 context sentence(design, population, supporting numbers)
Summary · context
We daily intraperitoneally injected male Wistar rats with a short mitochondrial peptide MOTS-c during 7-day unloading of their hind limbs.
Report a problemFND-4060824006
07AnimalAnimal study (in vivo)Mechanism
In rats during 7-day hindlimb unloading, MOTS‑c reduced MuRF mRNA compared with the unloading group given placebo.
In plain wordsIn rats, MOTS‑c lowered a muscle breakdown marker (MuRF mRNA) compared with unloaded rats given placebo.
Peptide
MOTS-c
Outcome
DecreaseMuRF mRNA in soleus
Study
ratn: not reported in source
7-day hindlimb unloading
Comparator
Hindlimb unloading with placebo
Outcome group
Muscle injury and repair · Musculoskeletal
Studied dose
Dose not stated · intraperitoneal · 7 daysAs given in this study (rat).
Source sentences
Summary
The ubiquitin ligases MuRF and Atrogin-1 mRNA were also reduced compared to the hindlimb unloading group with placebo.
1 context sentence(design, population, supporting numbers)
Summary · context
We daily intraperitoneally injected male Wistar rats with a short mitochondrial peptide MOTS-c during 7-day unloading of their hind limbs.
Report a problemFND-4060824007
08AnimalAnimal study (in vivo)Mechanism
In rats during 7-day hindlimb unloading, MOTS‑c reduced Atrogin-1 mRNA compared with the unloading group given placebo.
In plain wordsIn rats, MOTS‑c lowered a muscle breakdown marker (Atrogin-1 mRNA) compared with unloaded rats given placebo.
Peptide
MOTS-c
Outcome
DecreaseAtrogin-1 mRNA in soleus
Study
ratn: not reported in source
7-day hindlimb unloading
Comparator
Hindlimb unloading with placebo
Outcome group
Muscle injury and repair · Musculoskeletal
Studied dose
Dose not stated · intraperitoneal · 7 daysAs given in this study (rat).
Source sentences
Summary
The ubiquitin ligases MuRF and Atrogin-1 mRNA were also reduced compared to the hindlimb unloading group with placebo.
1 context sentence(design, population, supporting numbers)
Summary · context
We daily intraperitoneally injected male Wistar rats with a short mitochondrial peptide MOTS-c during 7-day unloading of their hind limbs.
Report a problemFND-4060824008
09AnimalAnimal study (in vivo)Mechanism
In rats during 7-day hindlimb unloading, MOTS‑c prevented the decrease in expression of some mitochondrial biogenesis parameters.
In plain wordsIn rats, MOTS‑c prevented the drop in some markers of new mitochondria formation during hindlimb unloading.
Peptide
MOTS-c
Outcome
IncreaseMitochondrial biogenesis parameters in soleus
Study
ratn: not reported in source
7-day hindlimb unloading
Comparator
Hindlimb unloading group
Outcome group
Mitochondrial function · Musculoskeletal
Studied dose
Dose not stated · intraperitoneal · 7 daysAs given in this study (rat).
Source sentences
Summary
MOTS-c prevented a decrease in the expression of a few mitochondrial biogenesis parameters
1 context sentence(design, population, supporting numbers)
Summary · context
We daily intraperitoneally injected male Wistar rats with a short mitochondrial peptide MOTS-c during 7-day unloading of their hind limbs.
Report a problemFND-4060824009
10AnimalAnimal study (in vivo)Mechanism
In rats during 7-day hindlimb unloading, MOTS‑c prevented the decrease in ACC phosphorylation, an AMPK target.
In plain wordsIn rats, MOTS‑c prevented the drop in ACC phosphorylation, a marker of AMPK activity, during hindlimb unloading.
Peptide
MOTS-c
Outcome
IncreaseACC phosphorylation (AMPK target) in soleus
Study
ratn: not reported in source
7-day hindlimb unloading
Comparator
Hindlimb unloading group
Outcome group
Mitochondrial function · Musculoskeletal
Studied dose
Dose not stated · intraperitoneal · 7 daysAs given in this study (rat).
Source sentences
Summary
MOTS-c prevented a decrease in the expression of a few mitochondrial biogenesis parameters and the level of ACC phosphorylation (AMPK target)
1 context sentence(design, population, supporting numbers)
Summary · context
We daily intraperitoneally injected male Wistar rats with a short mitochondrial peptide MOTS-c during 7-day unloading of their hind limbs.
Report a problemFND-4060824010

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
9 Oct 2026, 18:17 UTC
Study results updated
Version 1
9 Oct 2026, 02:47 UTC
First added · source update from Europe PMC

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Terms as the source lists them.

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