MOTS-c modulates pancreatic islet function in rats and pigs in vitro.

Bień J, Pruszynska-Oszmalek E, Kolodziejski P, Leciejewska N, Szczepankiewicz D, Grzęda E, Sassek M

Histochemistry and cell biology6 Jun 2025Cited by 1 (Europe PMC)

PMID40478460PMCIDPMC12144051DOI10.1007/s00418-025-02391-4PepBankPUB-40478460

Summary

MOTS-c is a promising regulator of metabolism and energy homeostasis. While its effects have been studied in cell lines, our team aimed to investigate its influence on more complex structures-specifically, isolated pancreatic islets. We used two animal models: the rat, which is commonly studied, and the pig, which shares greater physiological similarities with humans. This study assessed the expression and secretion of insulin and glucagon, the expression of their receptors, cell viability, and cell death following MOTS-c treatment of the islets. Additionally, we examined how MOTS-c secretion is affected by different incubation media, such as the presence of free fatty acids, pancreatic hormones, and different glucose concentrations. The results indicate that MOTS-c impacts pancreatic islet physiology by, for example, reducing insulin and glucagon secretion and enhancing cell viability. Notably, the effects differed between the two species, which may be attributed to anatomical differences in their pancreatic islets or structural variations in rat and pig MOTS-c. These facts may lead to the conclusion that if MOTS-c may be helpful in human medicine, the pig model should be considered another valuable choice.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study4

4 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueEfficacy outcome
In isolated rat and pig pancreatic islets, MOTS‑c reduced insulin secretion overall, but the abstract says the effects differed between the two species.
In plain wordsIn lab tests on rat and pig pancreatic islets, MOTS‑c reduced insulin release overall, but the effects differed between the two species.
Peptide
MOTS-c
Outcome
DecreaseInsulin secretion from islets
Study
rat and pign: not reported in source
isolated pancreatic islets
Outcome group
Glycemic control · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
The results indicate that MOTS-c impacts pancreatic islet physiology by, for example, reducing insulin and glucagon secretion and enhancing cell viability.
1 context sentence(design, population, supporting numbers)
Summary · context
We used two animal models: the rat, which is commonly studied, and the pig, which shares greater physiological similarities with humans.
Show in the study text Report a problemFND-4047846001
02LabEx vivo tissueEfficacy outcome
In isolated rat and pig pancreatic islets, MOTS‑c reduced glucagon secretion overall, but the abstract says the effects differed between the two species.
In plain wordsIn lab tests on rat and pig pancreatic islets, MOTS‑c reduced glucagon release overall, but the effects differed between the two species.
Peptide
MOTS-c
Outcome
DecreaseGlucagon secretion from islets
Study
rat and pign: not reported in source
isolated pancreatic islets
Outcome group
Glycemic control · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
The results indicate that MOTS-c impacts pancreatic islet physiology by, for example, reducing insulin and glucagon secretion and enhancing cell viability.
1 context sentence(design, population, supporting numbers)
Summary · context
We used two animal models: the rat, which is commonly studied, and the pig, which shares greater physiological similarities with humans.
Show in the study text Report a problemFND-4047846002
03LabEx vivo tissueEfficacy outcome
In isolated rat and pig pancreatic islets, MOTS‑c enhanced cell viability overall, but the abstract says the effects differed between the two species.
In plain wordsIn lab tests on rat and pig pancreatic islets, MOTS‑c improved cell survival overall, but the effects differed between the two species.
Peptide
MOTS-c
Outcome
IncreaseIslet cell viability
Study
rat and pign: not reported in source
isolated pancreatic islets
Outcome group
Glycemic control · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
The results indicate that MOTS-c impacts pancreatic islet physiology by, for example, reducing insulin and glucagon secretion and enhancing cell viability.
1 context sentence(design, population, supporting numbers)
Summary · context
We used two animal models: the rat, which is commonly studied, and the pig, which shares greater physiological similarities with humans.
Show in the study text Report a problemFND-4047846003
04LabEx vivo tissueMechanism
In isolated pancreatic islets, the effects of MOTS‑c differed between rat and pig.
In plain wordsIn lab tests on islets, MOTS‑c effects differed between rat and pig.
Peptide
MOTS-c
Outcome
MixedSpecies difference in islet response
Study
rat and pign: not reported in source
isolated pancreatic islets
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
Notably, the effects differed between the two species, which may be attributed to anatomical differences in their pancreatic islets or structural variations in rat and pig MOTS-c.
1 context sentence(design, population, supporting numbers)
Summary · context
We used two animal models: the rat, which is commonly studied, and the pig, which shares greater physiological similarities with humans.
Show in the study text Report a problemFND-4047846004

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
9 Oct 2026, 18:17 UTC
Study results updated
Version 1
9 Oct 2026, 02:47 UTC
First added · source update from Europe PMC

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