ACTH-like Peptides Compensate Rat Brain Gene Expression Profile Disrupted by Ischemia a Day After Experimental Stroke.

Filippenkov IB, Shpetko YY, Stavchansky VV, Denisova AE, Gubsky LV, Andreeva LA, Myasoedov NF, Limborska SA, Dergunova LV

Biomedicines13 Dec 2024Cited by 3 (Europe PMC)

PMID39767736PMCIDPMC11673339DOI10.3390/biomedicines12122830PepBankPUB-39767736

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Summary

Background: Ischemic stroke results from a disruption of cerebral blood flow. Adrenocorticotropic hormone (ACTH) serves as the basis for the creation of synthetic peptides as neuroprotective agents for stroke therapy. Previously, using RNA-Seq we first revealed differential expressed genes (DEGs) associated with ACTH(4-7)PGP (Semax) and ACTH(6-9)PGP peptides under cerebral ischemia conditions. Analysis was carried out at 4.5 h after transient middle cerebral artery occlusion (tMCAO) model in the ipsilateral frontal cortex of a rat brain. Methods: Here, we analyzed the penumbra-associated frontal cortex of rats and actions under the same peptides at 24 h after tMCAO using RNA-Seq. Results: 3774 DEGs (fold change > 1.5 and Padj < 0.05) were identified under ischemia conditions, whereas 1539 and 2066 DEGs were revealed under Semax and ACTH(6-9)PGP peptides at 24 h after tMCAO. Furthermore, both peptides significantly reduced expression distortions caused by ischemia for 1171 genes associated with immune and neurosignaling pathways. Concomitantly, there were 32 DEGs under ACTH(6-9)PGP versus Semax administration at 24 h after tMCAO. Besides, neurogenesis-, angiogenesis-, protein kinase- and growth factor-related DEGs were revealed under peptides action. Previously, we observed the neuroprotective effect of peptides at the histological level in rat brains at 24 h after tMCAO. Thus, here we demonstrate the transcriptome manifestation of this histological effect. Furthermore, comparison with previous data at the 4.5 h post-tMCAO time point showed that the pattern of peptide action on the transcriptome depends on the time elapsed after tMCAO. Conclusions: We revealed that the effect of ACTH(6-9)PGP was more similar to Semax than different from it a day after tMCAO. At this time point, ACTH-like peptides compensated rat brain gene expression profiles disrupted by ischemia. Thus, our results may be useful for selecting more effective structures for future anti-stroke drugs and appropriate post-stroke time points for their testing.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study4

4 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Mechanism
In rats 24 h after transient middle cerebral artery occlusion, Semax was linked to 1539 differentially expressed genes in the frontal cortex.
In plain wordsIn rats after an induced stroke, Semax changed the activity of 1539 genes in the brain.
Peptide
Semax
Outcome
Not applicableDifferentially expressed genes under Semax at 24 h
Study
ratn: not reported in source
tMCAO model, penumbra-associated frontal cortex, 24 h
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated
Measurements
Differentially expressed genes
1539 genes
Semax, 24 h after tMCAO
Source sentences
Summary
1539 and 2066 DEGs were revealed under Semax and ACTH(6-9)PGP peptides at 24 h after tMCAO.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we analyzed the penumbra-associated frontal cortex of rats and actions under the same peptides at 24 h after tMCAO using RNA-Seq.
Show in the study text Report a problemFND-3976773601
02AnimalAnimal study (in vivo)Mechanism
In rats 24 h after transient middle cerebral artery occlusion, Semax and ACTH(6-9)PGP significantly reduced ischemia-induced expression distortions for 1171 genes linked to immune and neurosignaling pathways.
In plain wordsIn rats after an induced stroke, Semax and a related peptide reduced stroke-caused gene activity changes in immune and nerve-signal genes.
Peptide
Semax
Outcome
DecreaseIschemia-induced expression distortions
Study
ratn: not reported in source
tMCAO model, penumbra-associated frontal cortex, 24 h
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated
Measurements
Genes with reduced expression distortion
1171 genes
both peptides
Source sentences
Summary
Furthermore, both peptides significantly reduced expression distortions caused by ischemia for 1171 genes associated with immune and neurosignaling pathways.
2 context sentences(design, population, supporting numbers)
Summary · context
1539 and 2066 DEGs were revealed under Semax and ACTH(6-9)PGP peptides at 24 h after tMCAO.
Summary · context
Here, we analyzed the penumbra-associated frontal cortex of rats and actions under the same peptides at 24 h after tMCAO using RNA-Seq.
Show in the study text Report a problemFND-3976773602
03AnimalAnimal study (in vivo)Mechanism
In rats 24 h after transient middle cerebral artery occlusion, 32 genes differed in expression between ACTH(6-9)PGP and Semax administration.
In plain wordsIn rats after an induced stroke, only 32 genes differed between treatment with ACTH(6-9)PGP and treatment with Semax.
Peptide
Semax
Outcome
Not applicableDifferentially expressed genes, ACTH(6-9)PGP versus Semax
Study
ratn: not reported in source
tMCAO model, penumbra-associated frontal cortex, 24 h
Comparator
ACTH(6-9)PGP
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated
Measurements
Differentially expressed genes
32 genes
ACTH(6-9)PGP versus Semax
Source sentences
Summary
Concomitantly, there were 32 DEGs under ACTH(6-9)PGP versus Semax administration at 24 h after tMCAO.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we analyzed the penumbra-associated frontal cortex of rats and actions under the same peptides at 24 h after tMCAO using RNA-Seq.
Show in the study text Report a problemFND-3976773603
04AnimalAnimal study (in vivo)Mechanism
In rats 24 h after transient middle cerebral artery occlusion, differentially expressed genes related to neurogenesis, angiogenesis, protein kinases and growth factors appeared under the action of the peptides, Semax among them.
In plain wordsIn rats after an induced stroke, the peptides, Semax among them, changed genes linked to nerve growth, blood vessel growth and growth factors.
Peptide
Semax
Outcome
Not applicableNeurogenesis, angiogenesis, protein kinase and growth factor genes
Study
ratn: not reported in source
tMCAO model, penumbra-associated frontal cortex, 24 h
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated
Source sentences
Summary
Besides, neurogenesis-, angiogenesis-, protein kinase- and growth factor-related DEGs were revealed under peptides action.
2 context sentences(design, population, supporting numbers)
Summary · context
1539 and 2066 DEGs were revealed under Semax and ACTH(6-9)PGP peptides at 24 h after tMCAO.
Summary · context
Here, we analyzed the penumbra-associated frontal cortex of rats and actions under the same peptides at 24 h after tMCAO using RNA-Seq.
Show in the study text Report a problemFND-3976773604

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Version 2Current
10 Oct 2026, 10:21 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

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