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Study · Animal study (in vivo) · rat · 2025

17 results

Protective effect of Dulaglutide, a GLP1 agonist, on acetic acid-induced ulcerative colitis in rats: involvement of GLP-1, TFF-3, and TGF-β/PI3K/NF-κB signaling pathway.

Mahdy RNE, Nader MA, Helal MG, Abu-Risha SE, Abdelmageed ME

Naunyn-Schmiedeberg's archives of pharmacology23 Nov 2024Cited by 18 (Europe PMC)

PMID39579211 (opens in a new tab)PMCIDPMC11985593 (opens in a new tab)DOI10.1007/s00210-024-03631-5 (opens in a new tab)PepBankPUB-39579211
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Summary

A chronic inflammatory condition of the colon called ulcerative colitis (UC) is characterized by mucosal surface irritation that extends from the rectum to the near proximal colon portions. The rationale of this work was to conclude if dulaglutide (Dula) could protect rats from developing colitis caused by exposure to acetic acid (AA). Rats were randomly divided into seven groups (each with eight rats): Normal control, Dula control, AA (received 2 milliliters of 3% v/v AA through the rectum), Sulfasalazine (SLZ); given SLZ (100 mg/kg) orally from day 11 to day 21 then AA intrarectally on day 22 and Dula groups ( pretreated with 50, 100 or 150 μg/kg subcutaneous injection of Dula - once weekly for three weeks and AA on day 22 to induce ulcerative colitis, colon tissues and blood samples were taken on day 23. By generating colonic histological deviations such as inflammatory processes, goblet cell death, glandular hyperplasia, and mucosa ulcers, Dula dropped AA-induced colitis. Additionally, these modifications diminished blood lactate dehydrogenase (LDH), C-reactive protein (CRP), colon weight, and the weight/length ratio of the colon. In addition, Dula decreased the oxidative stress biomarker malondialdehyde (MDA) and increased the antioxidant enzymes (total antioxidant capacity (TAC), reduced glutathione (GSH), and superoxide dismutase (SOD) concentrations). Dula also significantly reduced the expression of transforming growth factor-1 (TGF-β1), phosphatidylinositol-3-kinase (PI3K), protein kinase B (AKT) signaling pathway, and the inflammatory cytokines: nuclear factor kappa B (NF-κB), interleukin-6 (IL-6), and interferon-γ (IFN-γ) in colonic cellular structures. In addition, Dula enforced the levels of glucagon-like peptide-1 (GLP-1) and trefoil factor-3 (TFF-3) that were crucial to intestinal mucosa regeneration and healing of wounds. By modulating TGF-β1 in conjunction with other inflammatory pathways like PI3K/AKT and NF-κB, regulating the oxidant/antioxidant balance, and improving the integrity of the intestinal barrier, Dula prevented AA-induced colitis in rats.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study17

17 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In rats with acetic acid-induced colitis, dulaglutide reduced colitis with reported histological changes.

    In plain wordsIn rats with acid-induced colitis, dulaglutide reduced colitis compared with the acid-induced condition.

    Peptide
    Dulaglutide (Trulicity)
    Outcome
    DecreaseColitis histology
    Study

    ratn: not reported in source

    Acetic acid-induced colitis
    Comparator
    Acetic acid-induced colitis
    Outcome group
    Gastrointestinal protection · Gastrointestinal
    Studied dose
    50, 100 or 150 μg/kg once weekly · subcutaneous · three weeksAs given in this study (rat).

    Source sentences

    Summary
    By generating colonic histological deviations such as inflammatory processes, goblet cell death, glandular hyperplasia, and mucosa ulcers, Dula dropped AA-induced colitis.
    1 context sentence
    Summary · context
    Dula groups ( pretreated with 50, 100 or 150 μg/kg subcutaneous injection of Dula - once weekly for three weeks and AA on day 22 to induce ulcerative colitis, colon tissues and blood samples were taken on day 23.
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In rats with acetic acid-induced colitis, dulaglutide reduced blood lactate dehydrogenase.

    In plain wordsIn rats with acid-induced colitis, dulaglutide lowered blood lactate dehydrogenase compared with the acid-induced condition.

    Peptide
    Dulaglutide (Trulicity)
    Outcome
    DecreaseBlood lactate dehydrogenase
    Study

    ratn: not reported in source

    Acetic acid-induced colitis
    Comparator
    Acetic acid-induced colitis
    Outcome group
    Organ injury · Gastrointestinal
    Studied dose
    50, 100 or 150 μg/kg once weekly · subcutaneous · three weeksAs given in this study (rat).

    Source sentences

    Summary
    Additionally, these modifications diminished blood lactate dehydrogenase (LDH), C-reactive protein (CRP), colon weight, and the weight/length ratio of the colon.
    1 context sentence
    Summary · context
    Dula groups ( pretreated with 50, 100 or 150 μg/kg subcutaneous injection of Dula - once weekly for three weeks and AA on day 22 to induce ulcerative colitis, colon tissues and blood samples were taken on day 23.
  3. 03AnimalAnimal study (in vivo)Efficacy outcome

    In rats with acetic acid-induced colitis, dulaglutide reduced C-reactive protein.

    In plain wordsIn rats with acid-induced colitis, dulaglutide lowered C-reactive protein compared with the acid-induced condition.

    Peptide
    Dulaglutide (Trulicity)
    Outcome
    DecreaseC-reactive protein
    Study

    ratn: not reported in source

    Acetic acid-induced colitis
    Comparator
    Acetic acid-induced colitis
    Outcome group
    Inflammation markers · Immune and inflammation
    Studied dose
    50, 100 or 150 μg/kg once weekly · subcutaneous · three weeksAs given in this study (rat).

    Source sentences

    Summary
    Additionally, these modifications diminished blood lactate dehydrogenase (LDH), C-reactive protein (CRP), colon weight, and the weight/length ratio of the colon.
    1 context sentence
    Summary · context
    Dula groups ( pretreated with 50, 100 or 150 μg/kg subcutaneous injection of Dula - once weekly for three weeks and AA on day 22 to induce ulcerative colitis, colon tissues and blood samples were taken on day 23.
  4. 04AnimalAnimal study (in vivo)Efficacy outcome

    In rats with acetic acid-induced colitis, dulaglutide reduced colon weight.

    In plain wordsIn rats with acid-induced colitis, dulaglutide lowered colon weight compared with the acid-induced condition.

    Peptide
    Dulaglutide (Trulicity)
    Outcome
    DecreaseColon weight
    Study

    ratn: not reported in source

    Acetic acid-induced colitis
    Comparator
    Acetic acid-induced colitis
    Outcome group
    Gastrointestinal protection · Gastrointestinal
    Studied dose
    50, 100 or 150 μg/kg once weekly · subcutaneous · three weeksAs given in this study (rat).

    Source sentences

    Summary
    Additionally, these modifications diminished blood lactate dehydrogenase (LDH), C-reactive protein (CRP), colon weight, and the weight/length ratio of the colon.
    1 context sentence
    Summary · context
    Dula groups ( pretreated with 50, 100 or 150 μg/kg subcutaneous injection of Dula - once weekly for three weeks and AA on day 22 to induce ulcerative colitis, colon tissues and blood samples were taken on day 23.
  5. 05AnimalAnimal study (in vivo)Efficacy outcome

    In rats with acetic acid-induced colitis, dulaglutide reduced colon weight/length ratio.

    In plain wordsIn rats with acid-induced colitis, dulaglutide lowered the colon weight-to-length ratio compared with the acid-induced condition.

    Peptide
    Dulaglutide (Trulicity)
    Outcome
    DecreaseColon weight/length ratio
    Study

    ratn: not reported in source

    Acetic acid-induced colitis
    Comparator
    Acetic acid-induced colitis
    Outcome group
    Gastrointestinal protection · Gastrointestinal
    Studied dose
    50, 100 or 150 μg/kg once weekly · subcutaneous · three weeksAs given in this study (rat).

    Source sentences

    Summary
    Additionally, these modifications diminished blood lactate dehydrogenase (LDH), C-reactive protein (CRP), colon weight, and the weight/length ratio of the colon.
    1 context sentence
    Summary · context
    Dula groups ( pretreated with 50, 100 or 150 μg/kg subcutaneous injection of Dula - once weekly for three weeks and AA on day 22 to induce ulcerative colitis, colon tissues and blood samples were taken on day 23.
  6. 06AnimalAnimal study (in vivo)Efficacy outcome

    In rats with acetic acid-induced colitis, dulaglutide reduced malondialdehyde.

    In plain wordsIn rats with acid-induced colitis, dulaglutide lowered malondialdehyde compared with the acid-induced condition.

    Peptide
    Dulaglutide (Trulicity)
    Outcome
    DecreaseMalondialdehyde
    Study

    ratn: not reported in source

    Acetic acid-induced colitis
    Comparator
    Acetic acid-induced colitis
    Outcome group
    Oxidative stress · Gastrointestinal
    Studied dose
    50, 100 or 150 μg/kg once weekly · subcutaneous · three weeksAs given in this study (rat).

    Source sentences

    Summary
    In addition, Dula decreased the oxidative stress biomarker malondialdehyde (MDA) and increased the antioxidant enzymes (total antioxidant capacity (TAC), reduced glutathione (GSH), and superoxide dismutase (SOD) concentrations).
    1 context sentence
    Summary · context
    Dula groups ( pretreated with 50, 100 or 150 μg/kg subcutaneous injection of Dula - once weekly for three weeks and AA on day 22 to induce ulcerative colitis, colon tissues and blood samples were taken on day 23.
  7. 07AnimalAnimal study (in vivo)Efficacy outcome

    In rats with acetic acid-induced colitis, dulaglutide increased total antioxidant capacity.

    In plain wordsIn rats with acid-induced colitis, dulaglutide increased antioxidant capacity compared with the acid-induced condition.

    Peptide
    Dulaglutide (Trulicity)
    Outcome
    IncreaseTotal antioxidant capacity
    Study

    ratn: not reported in source

    Acetic acid-induced colitis
    Comparator
    Acetic acid-induced colitis
    Outcome group
    Oxidative stress · Gastrointestinal
    Studied dose
    50, 100 or 150 μg/kg once weekly · subcutaneous · three weeksAs given in this study (rat).

    Source sentences

    Summary
    In addition, Dula decreased the oxidative stress biomarker malondialdehyde (MDA) and increased the antioxidant enzymes (total antioxidant capacity (TAC), reduced glutathione (GSH), and superoxide dismutase (SOD) concentrations).
    1 context sentence
    Summary · context
    Dula groups ( pretreated with 50, 100 or 150 μg/kg subcutaneous injection of Dula - once weekly for three weeks and AA on day 22 to induce ulcerative colitis, colon tissues and blood samples were taken on day 23.
  8. 08AnimalAnimal study (in vivo)Efficacy outcome

    In rats with acetic acid-induced colitis, dulaglutide increased reduced glutathione.

    In plain wordsIn rats with acid-induced colitis, dulaglutide increased reduced glutathione compared with the acid-induced condition.

    Peptide
    Dulaglutide (Trulicity)
    Outcome
    IncreaseReduced glutathione
    Study

    ratn: not reported in source

    Acetic acid-induced colitis
    Comparator
    Acetic acid-induced colitis
    Outcome group
    Oxidative stress · Gastrointestinal
    Studied dose
    50, 100 or 150 μg/kg once weekly · subcutaneous · three weeksAs given in this study (rat).

    Source sentences

    Summary
    In addition, Dula decreased the oxidative stress biomarker malondialdehyde (MDA) and increased the antioxidant enzymes (total antioxidant capacity (TAC), reduced glutathione (GSH), and superoxide dismutase (SOD) concentrations).
    1 context sentence
    Summary · context
    Dula groups ( pretreated with 50, 100 or 150 μg/kg subcutaneous injection of Dula - once weekly for three weeks and AA on day 22 to induce ulcerative colitis, colon tissues and blood samples were taken on day 23.
  9. 09AnimalAnimal study (in vivo)Efficacy outcome

    In rats with acetic acid-induced colitis, dulaglutide increased superoxide dismutase.

    In plain wordsIn rats with acid-induced colitis, dulaglutide increased superoxide dismutase compared with the acid-induced condition.

    Peptide
    Dulaglutide (Trulicity)
    Outcome
    IncreaseSuperoxide dismutase
    Study

    ratn: not reported in source

    Acetic acid-induced colitis
    Comparator
    Acetic acid-induced colitis
    Outcome group
    Oxidative stress · Gastrointestinal
    Studied dose
    50, 100 or 150 μg/kg once weekly · subcutaneous · three weeksAs given in this study (rat).

    Source sentences

    Summary
    In addition, Dula decreased the oxidative stress biomarker malondialdehyde (MDA) and increased the antioxidant enzymes (total antioxidant capacity (TAC), reduced glutathione (GSH), and superoxide dismutase (SOD) concentrations).
    1 context sentence
    Summary · context
    Dula groups ( pretreated with 50, 100 or 150 μg/kg subcutaneous injection of Dula - once weekly for three weeks and AA on day 22 to induce ulcerative colitis, colon tissues and blood samples were taken on day 23.
  10. 10AnimalAnimal study (in vivo)Mechanism

    In rats with acetic acid-induced colitis, dulaglutide reduced colonic TGF-β1 expression.

    In plain wordsIn rats with acid-induced colitis, dulaglutide lowered colon TGF-β1 expression compared with the acid-induced condition.

    Peptide
    Dulaglutide (Trulicity)
    Outcome
    DecreaseTGF-β1 expression
    Study

    ratn: not reported in source

    Acetic acid-induced colitis
    Comparator
    Acetic acid-induced colitis
    Outcome group
    Receptor activity and mechanism · Gastrointestinal
    Studied dose
    50, 100 or 150 μg/kg once weekly · subcutaneous · three weeksAs given in this study (rat).

    Source sentences

    Summary
    Dula also significantly reduced the expression of transforming growth factor-1 (TGF-β1), phosphatidylinositol-3-kinase (PI3K), protein kinase B (AKT) signaling pathway, and the inflammatory cytokines: nuclear factor kappa B (NF-κB), interleukin-6 (IL-6), and interferon-γ (IFN-γ) in colonic cellular structures.
    1 context sentence
    Summary · context
    Dula groups ( pretreated with 50, 100 or 150 μg/kg subcutaneous injection of Dula - once weekly for three weeks and AA on day 22 to induce ulcerative colitis, colon tissues and blood samples were taken on day 23.
  11. 11AnimalAnimal study (in vivo)Mechanism

    In rats with acetic acid-induced colitis, dulaglutide reduced colonic PI3K expression.

    In plain wordsIn rats with acid-induced colitis, dulaglutide lowered colon PI3K expression compared with the acid-induced condition.

    Peptide
    Dulaglutide (Trulicity)
    Outcome
    DecreasePI3K expression
    Study

    ratn: not reported in source

    Acetic acid-induced colitis
    Comparator
    Acetic acid-induced colitis
    Outcome group
    Receptor activity and mechanism · Gastrointestinal
    Studied dose
    50, 100 or 150 μg/kg once weekly · subcutaneous · three weeksAs given in this study (rat).

    Source sentences

    Summary
    Dula also significantly reduced the expression of transforming growth factor-1 (TGF-β1), phosphatidylinositol-3-kinase (PI3K), protein kinase B (AKT) signaling pathway, and the inflammatory cytokines: nuclear factor kappa B (NF-κB), interleukin-6 (IL-6), and interferon-γ (IFN-γ) in colonic cellular structures.
    1 context sentence
    Summary · context
    Dula groups ( pretreated with 50, 100 or 150 μg/kg subcutaneous injection of Dula - once weekly for three weeks and AA on day 22 to induce ulcerative colitis, colon tissues and blood samples were taken on day 23.
  12. 12AnimalAnimal study (in vivo)Mechanism

    In rats with acetic acid-induced colitis, dulaglutide reduced colonic AKT signaling.

    In plain wordsIn rats with acid-induced colitis, dulaglutide reduced colon AKT signaling compared with the acid-induced condition.

    Peptide
    Dulaglutide (Trulicity)
    Outcome
    DecreaseAKT signaling
    Study

    ratn: not reported in source

    Acetic acid-induced colitis
    Comparator
    Acetic acid-induced colitis
    Outcome group
    Receptor activity and mechanism · Gastrointestinal
    Studied dose
    50, 100 or 150 μg/kg once weekly · subcutaneous · three weeksAs given in this study (rat).

    Source sentences

    Summary
    Dula also significantly reduced the expression of transforming growth factor-1 (TGF-β1), phosphatidylinositol-3-kinase (PI3K), protein kinase B (AKT) signaling pathway, and the inflammatory cytokines: nuclear factor kappa B (NF-κB), interleukin-6 (IL-6), and interferon-γ (IFN-γ) in colonic cellular structures.
    1 context sentence
    Summary · context
    Dula groups ( pretreated with 50, 100 or 150 μg/kg subcutaneous injection of Dula - once weekly for three weeks and AA on day 22 to induce ulcerative colitis, colon tissues and blood samples were taken on day 23.
  13. 13AnimalAnimal study (in vivo)Efficacy outcome

    In rats with acetic acid-induced colitis, dulaglutide reduced colonic NF-κB expression.

    In plain wordsIn rats with acid-induced colitis, dulaglutide lowered colon NF-κB expression compared with the acid-induced condition.

    Peptide
    Dulaglutide (Trulicity)
    Outcome
    DecreaseNF-κB expression
    Study

    ratn: not reported in source

    Acetic acid-induced colitis
    Comparator
    Acetic acid-induced colitis
    Outcome group
    Inflammation markers · Immune and inflammation
    Studied dose
    50, 100 or 150 μg/kg once weekly · subcutaneous · three weeksAs given in this study (rat).

    Source sentences

    Summary
    Dula also significantly reduced the expression of transforming growth factor-1 (TGF-β1), phosphatidylinositol-3-kinase (PI3K), protein kinase B (AKT) signaling pathway, and the inflammatory cytokines: nuclear factor kappa B (NF-κB), interleukin-6 (IL-6), and interferon-γ (IFN-γ) in colonic cellular structures.
    1 context sentence
    Summary · context
    Dula groups ( pretreated with 50, 100 or 150 μg/kg subcutaneous injection of Dula - once weekly for three weeks and AA on day 22 to induce ulcerative colitis, colon tissues and blood samples were taken on day 23.
  14. 14AnimalAnimal study (in vivo)Efficacy outcome

    In rats with acetic acid-induced colitis, dulaglutide reduced colonic IL-6 expression.

    In plain wordsIn rats with acid-induced colitis, dulaglutide lowered colon IL-6 expression compared with the acid-induced condition.

    Peptide
    Dulaglutide (Trulicity)
    Outcome
    DecreaseIL-6 expression
    Study

    ratn: not reported in source

    Acetic acid-induced colitis
    Comparator
    Acetic acid-induced colitis
    Outcome group
    Inflammation markers · Immune and inflammation
    Studied dose
    50, 100 or 150 μg/kg once weekly · subcutaneous · three weeksAs given in this study (rat).

    Source sentences

    Summary
    Dula also significantly reduced the expression of transforming growth factor-1 (TGF-β1), phosphatidylinositol-3-kinase (PI3K), protein kinase B (AKT) signaling pathway, and the inflammatory cytokines: nuclear factor kappa B (NF-κB), interleukin-6 (IL-6), and interferon-γ (IFN-γ) in colonic cellular structures.
    1 context sentence
    Summary · context
    Dula groups ( pretreated with 50, 100 or 150 μg/kg subcutaneous injection of Dula - once weekly for three weeks and AA on day 22 to induce ulcerative colitis, colon tissues and blood samples were taken on day 23.
  15. 15AnimalAnimal study (in vivo)Efficacy outcome

    In rats with acetic acid-induced colitis, dulaglutide reduced colonic IFN-γ expression.

    In plain wordsIn rats with acid-induced colitis, dulaglutide lowered colon IFN-γ expression compared with the acid-induced condition.

    Peptide
    Dulaglutide (Trulicity)
    Outcome
    DecreaseIFN-γ expression
    Study

    ratn: not reported in source

    Acetic acid-induced colitis
    Comparator
    Acetic acid-induced colitis
    Outcome group
    Inflammation markers · Immune and inflammation
    Studied dose
    50, 100 or 150 μg/kg once weekly · subcutaneous · three weeksAs given in this study (rat).

    Source sentences

    Summary
    Dula also significantly reduced the expression of transforming growth factor-1 (TGF-β1), phosphatidylinositol-3-kinase (PI3K), protein kinase B (AKT) signaling pathway, and the inflammatory cytokines: nuclear factor kappa B (NF-κB), interleukin-6 (IL-6), and interferon-γ (IFN-γ) in colonic cellular structures.
    1 context sentence
    Summary · context
    Dula groups ( pretreated with 50, 100 or 150 μg/kg subcutaneous injection of Dula - once weekly for three weeks and AA on day 22 to induce ulcerative colitis, colon tissues and blood samples were taken on day 23.
  16. 16AnimalAnimal study (in vivo)Mechanism

    In rats with acetic acid-induced colitis, dulaglutide increased GLP-1 levels.

    In plain wordsIn rats with acid-induced colitis, dulaglutide increased GLP-1 levels compared with the acid-induced condition.

    Peptide
    Dulaglutide (Trulicity)
    Outcome
    IncreaseGLP-1 levels
    Study

    ratn: not reported in source

    Acetic acid-induced colitis
    Comparator
    Acetic acid-induced colitis
    Outcome group
    Receptor activity and mechanism · Gastrointestinal
    Studied dose
    50, 100 or 150 μg/kg once weekly · subcutaneous · three weeksAs given in this study (rat).

    Source sentences

    Summary
    In addition, Dula enforced the levels of glucagon-like peptide-1 (GLP-1) and trefoil factor-3 (TFF-3) that were crucial to intestinal mucosa regeneration and healing of wounds.
    1 context sentence
    Summary · context
    Dula groups ( pretreated with 50, 100 or 150 μg/kg subcutaneous injection of Dula - once weekly for three weeks and AA on day 22 to induce ulcerative colitis, colon tissues and blood samples were taken on day 23.
  17. 17AnimalAnimal study (in vivo)Mechanism

    In rats with acetic acid-induced colitis, dulaglutide increased TFF-3 levels.

    In plain wordsIn rats with acid-induced colitis, dulaglutide increased TFF-3 levels compared with the acid-induced condition.

    Peptide
    Dulaglutide (Trulicity)
    Outcome
    IncreaseTFF-3 levels
    Study

    ratn: not reported in source

    Acetic acid-induced colitis
    Comparator
    Acetic acid-induced colitis
    Outcome group
    Receptor activity and mechanism · Gastrointestinal
    Studied dose
    50, 100 or 150 μg/kg once weekly · subcutaneous · three weeksAs given in this study (rat).

    Source sentences

    Summary
    In addition, Dula enforced the levels of glucagon-like peptide-1 (GLP-1) and trefoil factor-3 (TFF-3) that were crucial to intestinal mucosa regeneration and healing of wounds.
    1 context sentence
    Summary · context
    Dula groups ( pretreated with 50, 100 or 150 μg/kg subcutaneous injection of Dula - once weekly for three weeks and AA on day 22 to induce ulcerative colitis, colon tissues and blood samples were taken on day 23.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    22 Oct 2026, 02:25 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 18:59 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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