Pichia pastoris secreted peptides crossing the blood-brain barrier and DSIP fusion peptide efficacy in PCPA-induced insomnia mouse models.

Mu X, Qu L, Yin L, Wang L, Liu X, Liu D

Frontiers in pharmacology8 Oct 2024Cited by 1 (Europe PMC)

PMID39444618PMCIDPMC11498945DOI10.3389/fphar.2024.1439536PepBankPUB-39444618

Summary

BackgroundPichia pastoris-secreted delta sleep inducing peptide and crossing the blood-brain barrier peptides (DSIP-CBBBP) fusion peptides holds significant promise for its potential sleep-enhancing and neurotransmitter balancing effects. This study investigates these properties using a p-chlorophenylalanine (PCPA) -induced insomnia model in mice, an approach akin to traditional methods evaluating sleep-promoting activities in fusion peptides.Aim of the studyThe research aims to elucidate the sleep-promoting mechanism of DSIP-CBBBP, exploring its impact on neurotransmitter levels and sleep regulation, and to analyze its composition and structure.Materials and methodsUsing a PCPA-induced insomnia mouse model, the study evaluates the sleep-promoting effects of DSIP-CBBBP. The peptide's influence on neurotransmitters such as 5-HT, glutamate, dopamine, and melatonin is assessed. The functions of DSIP-CBBBP are characterized using biochemical and animal insomnia-induced behavior tests and compared without CBBBP.ResultsDSIP-CBBBP demonstrates a capacity to modulate neurotransmitter levels, indicated by changes in 5-HT, glutamate, DA, and melatonin. DSIP-CBBBP shows a better restorative effect than DSIP on neurotransmitter imbalance and the potential to enhance sleep.ConclusionThe study underscores DSIP-CBBBP potential in correcting neurotransmitter dysregulation and promoting sleep, hinting at its utility in sleep-related therapies.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study5

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone5

In this study, Delta sleep-inducing peptide (DSIP) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Delta sleep-inducing peptide (DSIP).

01AnimalNot a test of Delta sleep-inducing peptide (DSIP) aloneAnimal study (in vivo)Mechanism
In PCPA-induced insomnia mice, the DSIP-CBBBP fusion peptide changed 5-HT levels; the direction is not stated.
In plain wordsIn mice with induced insomnia, a DSIP fusion peptide changed serotonin levels.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
Direction not reported5-HT level change
Study
mousen: not reported in source
PCPA-induced insomnia model
Outcome group
Neurotransmitters and neurotrophic factors · Nervous system and cognition
Source sentences
Summary
DSIP-CBBBP demonstrates a capacity to modulate neurotransmitter levels, indicated by changes in 5-HT, glutamate, DA, and melatonin.
1 context sentence(design, population, supporting numbers)
Summary · context
Using a PCPA-induced insomnia mouse model, the study evaluates the sleep-promoting effects of DSIP-CBBBP.
In this study, Delta sleep-inducing peptide (DSIP) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Delta sleep-inducing peptide (DSIP).
Show in the study text Report a problemFND-3944461801
02AnimalNot a test of Delta sleep-inducing peptide (DSIP) aloneAnimal study (in vivo)Mechanism
In PCPA-induced insomnia mice, the DSIP-CBBBP fusion peptide changed glutamate levels; the direction is not stated.
In plain wordsIn mice with induced insomnia, a DSIP fusion peptide changed glutamate levels.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
Direction not reportedGlutamate level change
Study
mousen: not reported in source
PCPA-induced insomnia model
Outcome group
Neurotransmitters and neurotrophic factors · Nervous system and cognition
Source sentences
Summary
DSIP-CBBBP demonstrates a capacity to modulate neurotransmitter levels, indicated by changes in 5-HT, glutamate, DA, and melatonin.
1 context sentence(design, population, supporting numbers)
Summary · context
Using a PCPA-induced insomnia mouse model, the study evaluates the sleep-promoting effects of DSIP-CBBBP.
In this study, Delta sleep-inducing peptide (DSIP) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Delta sleep-inducing peptide (DSIP).
Show in the study text Report a problemFND-3944461802
03AnimalNot a test of Delta sleep-inducing peptide (DSIP) aloneAnimal study (in vivo)Mechanism
In PCPA-induced insomnia mice, the DSIP-CBBBP fusion peptide changed dopamine levels; the direction is not stated.
In plain wordsIn mice with induced insomnia, a DSIP fusion peptide changed dopamine levels.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
Direction not reportedDopamine level change
Study
mousen: not reported in source
PCPA-induced insomnia model
Outcome group
Neurotransmitters and neurotrophic factors · Nervous system and cognition
Source sentences
Summary
DSIP-CBBBP demonstrates a capacity to modulate neurotransmitter levels, indicated by changes in 5-HT, glutamate, DA, and melatonin.
1 context sentence(design, population, supporting numbers)
Summary · context
Using a PCPA-induced insomnia mouse model, the study evaluates the sleep-promoting effects of DSIP-CBBBP.
In this study, Delta sleep-inducing peptide (DSIP) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Delta sleep-inducing peptide (DSIP).
Show in the study text Report a problemFND-3944461803
04AnimalNot a test of Delta sleep-inducing peptide (DSIP) aloneAnimal study (in vivo)Mechanism
In PCPA-induced insomnia mice, the DSIP-CBBBP fusion peptide changed melatonin levels; the direction is not stated.
In plain wordsIn mice with induced insomnia, a DSIP fusion peptide changed melatonin levels.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
Direction not reportedMelatonin level change
Study
mousen: not reported in source
PCPA-induced insomnia model
Outcome group
Neurotransmitters and neurotrophic factors · Nervous system and cognition
Source sentences
Summary
DSIP-CBBBP demonstrates a capacity to modulate neurotransmitter levels, indicated by changes in 5-HT, glutamate, DA, and melatonin.
1 context sentence(design, population, supporting numbers)
Summary · context
Using a PCPA-induced insomnia mouse model, the study evaluates the sleep-promoting effects of DSIP-CBBBP.
In this study, Delta sleep-inducing peptide (DSIP) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Delta sleep-inducing peptide (DSIP).
Show in the study text Report a problemFND-3944461804
05AnimalNot a test of Delta sleep-inducing peptide (DSIP) aloneAnimal study (in vivo)Efficacy outcome
In PCPA-induced insomnia mice, the DSIP-CBBBP fusion peptide restored neurotransmitter imbalance better than DSIP.
In plain wordsIn mice with induced insomnia, a DSIP fusion peptide corrected brain chemical imbalance better than DSIP did.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
IncreaseRestoration of neurotransmitter imbalance versus DSIP
Study
mousen: not reported in source
PCPA-induced insomnia model
Comparator
DSIP
Outcome group
Neurotransmitters and neurotrophic factors · Nervous system and cognition
Source sentences
Summary
DSIP-CBBBP shows a better restorative effect than DSIP on neurotransmitter imbalance and the potential to enhance sleep.
1 context sentence(design, population, supporting numbers)
Summary · context
Using a PCPA-induced insomnia mouse model, the study evaluates the sleep-promoting effects of DSIP-CBBBP.
In this study, Delta sleep-inducing peptide (DSIP) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Delta sleep-inducing peptide (DSIP).
Show in the study text Report a problemFND-3944461805

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
10 Oct 2026, 08:55 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

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