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Study · Animal study (in vivo) · mouse · 2024

5 results

FOXO4-DRI improves spermatogenesis in aged mice through reducing senescence-associated secretory phenotype secretion from Leydig cells.

Li Y, Zhang C, Cheng H, Lv L, Zhu X, Ma M, Xu Z, He J, Xie Y, Yang X, Liang X, Deng C, Liu G

Experimental gerontology19 Jul 2024Cited by 11 (Europe PMC)

PMID39025385 (opens in a new tab)DOI10.1016/j.exger.2024.112522 (opens in a new tab)PepBankPUB-39025385
Publisher pagePubMedAsk about this study
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Summary

Male ageing is always accompanied by decreased fertility. The forkhead O (FOXO) transcription factor FOXO4 is reported to be highly expressed in senescent cells. Upon activation, it binds p53 in the nucleus, preventing senescent cell apoptosis and maintaining senescent cells in situ. Leydig cells play key roles in assisting spermatogenesis. Leydig cell senescence leads to deterioration of the microenvironment of the testes and impairs spermatogenesis. In this study, we observed that FOXO4-DRI, a specific FOXO4- p53 binding blocker, induced apoptosis in senescent Leydig cells, reduced the secretion of certain Senescence-Associated Secretory Phenotype and improved the proliferation of cocultured GC-1 SPG cells. In naturally aged mice, FOXO4-DRI-treated aged mice exhibited increased sperm quality and improved spermatogenesis.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study5

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In naturally aged mice, FOXO4‑DRI treatment increased sperm quality.

    In plain wordsIn aged mice, FOXO4‑DRI treatment improved sperm quality.

    Peptide
    FOXO4-DRI
    Outcome
    IncreaseSperm quality
    Study

    mousen: not reported in source

    Naturally aged mice
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In naturally aged mice, FOXO4-DRI-treated aged mice exhibited increased sperm quality and improved spermatogenesis.
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In naturally aged mice, FOXO4‑DRI treatment improved spermatogenesis.

    In plain wordsIn aged mice, FOXO4‑DRI treatment improved sperm production.

    Peptide
    FOXO4-DRI
    Outcome
    IncreaseSpermatogenesis
    Study

    mousen: not reported in source

    Naturally aged mice
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In naturally aged mice, FOXO4-DRI-treated aged mice exhibited increased sperm quality and improved spermatogenesis.
  3. 03LabIn vitro (cells)Efficacy outcome

    In senescent Leydig cells, FOXO4‑DRI induced apoptosis.

    In plain wordsIn cells, FOXO4‑DRI caused aged Leydig cells to die.

    Peptide
    FOXO4-DRI
    Outcome
    IncreaseApoptosis of senescent Leydig cells
    Study

    n: not reported in source

    Senescent Leydig cells
    Outcome group
    Cell viability and death · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In this study, we observed that FOXO4-DRI, a specific FOXO4- p53 binding blocker, induced apoptosis in senescent Leydig cells, reduced the secretion of certain Senescence-Associated Secretory Phenotype and improved the proliferation of cocultured GC-1 SPG cells.
  4. 04LabIn vitro (cells)Efficacy outcome

    In senescent Leydig cells, FOXO4‑DRI reduced the secretion of certain senescence-associated secretory phenotype factors.

    In plain wordsIn cells, FOXO4‑DRI reduced the release of some aging-related signals from Leydig cells.

    Peptide
    FOXO4-DRI
    Outcome
    DecreaseSenescence-associated secretory phenotype secretion
    Study

    n: not reported in source

    Senescent Leydig cells
    Outcome group
    Aging markers · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In this study, we observed that FOXO4-DRI, a specific FOXO4- p53 binding blocker, induced apoptosis in senescent Leydig cells, reduced the secretion of certain Senescence-Associated Secretory Phenotype and improved the proliferation of cocultured GC-1 SPG cells.
  5. 05LabIn vitro (cells)Efficacy outcome

    In coculture with senescent Leydig cells, FOXO4‑DRI improved the proliferation of GC-1 SPG cells.

    In plain wordsIn cells, FOXO4‑DRI improved the growth of sperm precursor cells in coculture with Leydig cells.

    Peptide
    FOXO4-DRI
    Outcome
    IncreaseProliferation of cocultured GC-1 SPG cells
    Study

    n: not reported in source

    Senescent Leydig cells cocultured with GC-1 SPG cells
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In this study, we observed that FOXO4-DRI, a specific FOXO4- p53 binding blocker, induced apoptosis in senescent Leydig cells, reduced the secretion of certain Senescence-Associated Secretory Phenotype and improved the proliferation of cocultured GC-1 SPG cells.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

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