In mares resistant to endometrial infection, periovulatory treatment with ecbolic drugs does not influence uterine clearance or luteal development.

Khan Y, El-Shalofy A, Kaps M, Gautier C, Aurich C

Animal reproduction science1 Jul 2024

PMID38959672DOI10.1016/j.anireprosci.2024.107548PepBankPUB-38959672

Publisher page PubMedAsk about this study

Summary

We aimed to determine associations between experimentally impaired uterine clearance or treatment with ecbolic drugs on luteal development in estrous mares after insemination. In a crossover design, eight mares were treated with saline (CON), clenbuterol (CLEN), oxytocin (OXY) and carbetocin (CARB) from the day of first insemination until 2 days after ovulation. Between treatments, the mares rested for one cycle. Estrous mares were examined for the presence of free intrauterine fluid by transrectal ultrasound. Endometrial swabs for cytology and bacteriology were collected on days 1 and 14. Blood samples were collected daily before AI until day 14 after ovulation for determination of progesterone and PGF_2α metabolites (PGFM). Differences between treatments were compared by a general linear model for repeated measures (SPSS 29). One mare was excluded because of a uterine infection in the control cycle. In all other mares, only minor amounts of free intrauterine fluid were present after insemination and decreased over time (P<0.05) with no treatment x time interaction. There was no effect of treatment on polymorphonucleated cells (PMN) in endometrial cytology after ovulation or PGFM secretion. Progesterone release from day 1-14 as well as pregnancy rate and conceptus size on day 14 was not influenced by treatment. In conclusion, treatment with clenbuterol does not impair uterine clearance in estrous mares resistant to endometritis. Repeated injection of the oxytocin analogue carbetocin during the early postovulatory period is not detrimental to corpus luteum function and can be recommended to enhance uterine clearance.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study6

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone6

In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Oxytocin.

01AnimalNot a test of Oxytocin aloneAnimal study (in vivo)Efficacy outcome
In estrous mares, free intrauterine fluid decreased over time with no treatment-by-time interaction across saline, clenbuterol, oxytocin and carbetocin.
In plain wordsIn mares, fluid in the womb decreased over time, and the drug treatments, including oxytocin, did not change this pattern.
Peptide
Oxytocin
Outcome
No significant changeFree intrauterine fluid by treatment over time
Study
horsen = 7
Estrous mares
Comparator
Saline (CON)
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
In all other mares, only minor amounts of free intrauterine fluid were present after insemination and decreased over time (P<0.05) with no treatment x time interaction.
1 context sentence(design, population, supporting numbers)
Summary · context
In a crossover design, eight mares were treated with saline (CON), clenbuterol (CLEN), oxytocin (OXY) and carbetocin (CARB) from the day of first insemination until 2 days after ovulation.
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Show in the study text Report a problemFND-3895967201
02AnimalNot a test of Oxytocin aloneAnimal study (in vivo)Efficacy outcome
In estrous mares, treatment with clenbuterol, oxytocin or carbetocin had no effect on polymorphonucleated cells in endometrial cytology after ovulation.
In plain wordsIn mares, the drug treatments, including oxytocin, did not change white blood cells in womb samples.
Peptide
Oxytocin
Outcome
No significant changePolymorphonucleated cells in endometrial cytology
Study
horsen = 7
Estrous mares
Comparator
Saline (CON)
Outcome group
Inflammation markers · Reproductive and sexual health
Source sentences
Summary
There was no effect of treatment on polymorphonucleated cells (PMN) in endometrial cytology after ovulation
1 context sentence(design, population, supporting numbers)
Summary · context
In a crossover design, eight mares were treated with saline (CON), clenbuterol (CLEN), oxytocin (OXY) and carbetocin (CARB) from the day of first insemination until 2 days after ovulation.
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Show in the study text Report a problemFND-3895967202
03AnimalNot a test of Oxytocin aloneAnimal study (in vivo)Efficacy outcome
In estrous mares, treatment with clenbuterol, oxytocin or carbetocin had no effect on PGFM secretion.
In plain wordsIn mares, the drug treatments, including oxytocin, did not change a prostaglandin marker in blood.
Peptide
Oxytocin
Outcome
No significant changePGF2alpha metabolite (PGFM) secretion
Study
horsen = 7
Estrous mares
Comparator
Saline (CON)
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
or PGFM secretion.
1 context sentence(design, population, supporting numbers)
Summary · context
In a crossover design, eight mares were treated with saline (CON), clenbuterol (CLEN), oxytocin (OXY) and carbetocin (CARB) from the day of first insemination until 2 days after ovulation.
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Show in the study text Report a problemFND-3895967203
04AnimalNot a test of Oxytocin aloneAnimal study (in vivo)Efficacy outcome
In estrous mares, progesterone release from day 1 to 14 was not influenced by treatment.
In plain wordsIn mares, the drug treatments, including oxytocin, did not change progesterone release.
Peptide
Oxytocin
Outcome
No significant changeProgesterone release from day 1 to 14
Study
horsen = 7
Estrous mares
Comparator
Saline (CON)
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Progesterone release from day 1-14
1 context sentence(design, population, supporting numbers)
Summary · context
In a crossover design, eight mares were treated with saline (CON), clenbuterol (CLEN), oxytocin (OXY) and carbetocin (CARB) from the day of first insemination until 2 days after ovulation.
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Show in the study text Report a problemFND-3895967204
05AnimalNot a test of Oxytocin aloneAnimal study (in vivo)Efficacy outcome
In estrous mares, pregnancy rate on day 14 was not influenced by treatment.
In plain wordsIn mares, the drug treatments, including oxytocin, did not change pregnancy rate.
Peptide
Oxytocin
Outcome
No significant changePregnancy rate on day 14
Study
horsen = 7
Estrous mares
Comparator
Saline (CON)
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
pregnancy rate and conceptus size on day 14 was not influenced by treatment.
1 context sentence(design, population, supporting numbers)
Summary · context
In a crossover design, eight mares were treated with saline (CON), clenbuterol (CLEN), oxytocin (OXY) and carbetocin (CARB) from the day of first insemination until 2 days after ovulation.
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Show in the study text Report a problemFND-3895967205
06AnimalNot a test of Oxytocin aloneAnimal study (in vivo)Efficacy outcome
In estrous mares, conceptus size on day 14 was not influenced by treatment.
In plain wordsIn mares, the drug treatments, including oxytocin, did not change embryo size.
Peptide
Oxytocin
Outcome
No significant changeConceptus size on day 14
Study
horsen = 7
Estrous mares
Comparator
Saline (CON)
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
pregnancy rate and conceptus size on day 14 was not influenced by treatment.
1 context sentence(design, population, supporting numbers)
Summary · context
In a crossover design, eight mares were treated with saline (CON), clenbuterol (CLEN), oxytocin (OXY) and carbetocin (CARB) from the day of first insemination until 2 days after ovulation.
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Show in the study text Report a problemFND-3895967206

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
11 Oct 2026, 00:50 UTC
Study results updated
Version 1
9 Oct 2026, 02:48 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.