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Study · In vitro (cells) · 2024

4 results

Neuroprotection by Cerebrolysin and Citicoline Through the Upregulation of Brain-Derived Neurotrophic Factor (BDNF) Expression in the Affected Neural Cells: A Preliminary Clue Obtained Through an In Vitro Study.

P A, Rengarajan S, Venkatachalam S, Pattabi S, Jones S, K P, Krishna V, Prasanth K

Cureus21 Feb 2024Cited by 4 (Europe PMC)

PMID38524067 (opens in a new tab)PMCIDPMC10960614 (opens in a new tab)DOI10.7759/cureus.54665 (opens in a new tab)PepBankPUB-38524067
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Summary

ObjectivesCiticoline and cerebrolysin are two unique yet contentious medications because of inconsistencies in efficacy as well as the mystery surrounding their mode of action. The current study aimed to re-validate the neuroprotective benefits of these medications and investigate the possible molecular mechanism.MethodsNeuro-2A cells were exposed to tert-butyl hydroperoxide, a consistent in vitro model of neuronal damage caused by oxidative stress. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, acridine orange/ethidium bromide (AO-EtBr) staining, and phase-view examinations were utilized to evaluate cell survival and cytotoxicity. Real-time reverse transcription-polymerase chain reaction (RT-PCR)-based gene expression studies were conducted.Key findingObservations revealed that these two medications had modest but considerable neuroprotective effects. While the majority of the genes' expressions remained unchanged, cerebrolysin upregulated Neuregulin 1, and both upregulated brain-derived neurotrophic factor (BDNF) expression.ConclusionThe findings of the current study may be the first to suggest that citicoline and cerebrolysin may increase host cells' defense mechanisms (secretion neurotrophic factors) rather than carrying nutrients for cell survival. Because of its simplicity, the current study can readily be repeated to learn more about these two disputed medications for treating ischemic stroke.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study4

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    In damaged Neuro-2A cells, cerebrolysin showed modest but considerable neuroprotective effects.

    In plain wordsIn cells exposed to oxidative damage, cerebrolysin showed modest neuroprotective effects.

    Peptide
    Cerebrolysin
    Outcome
    IncreaseNeuroprotection
    Study

    n: not reported in source

    Neuro-2A cells exposed to tert-butyl hydroperoxide
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Observations revealed that these two medications had modest but considerable neuroprotective effects.
    1 context sentence
    Summary · context
    Neuro-2A cells were exposed to tert-butyl hydroperoxide, a consistent in vitro model of neuronal damage caused by oxidative stress.
  2. 02LabIn vitro (cells)Mechanism

    In damaged Neuro-2A cells, cerebrolysin increased Neuregulin 1 expression.

    In plain wordsIn cells exposed to oxidative damage, cerebrolysin increased Neuregulin 1 expression.

    Peptide
    Cerebrolysin
    Outcome
    IncreaseNeuregulin 1 expression
    Study

    n: not reported in source

    Neuro-2A cells exposed to tert-butyl hydroperoxide
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    While the majority of the genes' expressions remained unchanged, cerebrolysin upregulated Neuregulin 1, and both upregulated brain-derived neurotrophic factor (BDNF) expression.
    1 context sentence
    Summary · context
    Neuro-2A cells were exposed to tert-butyl hydroperoxide, a consistent in vitro model of neuronal damage caused by oxidative stress.
  3. 03LabIn vitro (cells)Mechanism

    In damaged Neuro-2A cells, cerebrolysin increased BDNF expression.

    In plain wordsIn cells exposed to oxidative damage, cerebrolysin increased BDNF expression.

    Peptide
    Cerebrolysin
    Outcome
    IncreaseBDNF expression
    Study

    n: not reported in source

    Neuro-2A cells exposed to tert-butyl hydroperoxide
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    While the majority of the genes' expressions remained unchanged, cerebrolysin upregulated Neuregulin 1, and both upregulated brain-derived neurotrophic factor (BDNF) expression.
    1 context sentence
    Summary · context
    Neuro-2A cells were exposed to tert-butyl hydroperoxide, a consistent in vitro model of neuronal damage caused by oxidative stress.
  4. 04LabIn vitro (cells)Mechanism

    In damaged Neuro-2A cells treated with cerebrolysin, most measured gene expression remained unchanged.

    In plain wordsIn cells exposed to oxidative damage, cerebrolysin left most measured gene expression unchanged.

    Peptide
    Cerebrolysin
    Outcome
    No significant changeOther gene expression
    Study

    n: not reported in source

    Neuro-2A cells exposed to tert-butyl hydroperoxide
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    While the majority of the genes' expressions remained unchanged, cerebrolysin upregulated Neuregulin 1, and both upregulated brain-derived neurotrophic factor (BDNF) expression.
    1 context sentence
    Summary · context
    Neuro-2A cells were exposed to tert-butyl hydroperoxide, a consistent in vitro model of neuronal damage caused by oxidative stress.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    14 Oct 2026, 05:02 UTC

    Study results updated

  2. Version 1

    14 Oct 2026, 01:46 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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