Skip to content

Study · In vitro (cells) · 2024

7 results

MOTS-c regulates pancreatic alpha and beta cell functions in vitro.

Bień J, Pruszyńska-Oszmałek E, Kołodziejski P, Leciejewska N, Szczepankiewicz D, Sassek M

Histochemistry and cell biology2 Mar 2024Cited by 6 (Europe PMC)

PMID38430258 (opens in a new tab)DOI10.1007/s00418-024-02274-0 (opens in a new tab)PepBankPUB-38430258
Publisher pagePubMedAsk about this study
Jump to 7 results

Summary

The aim of this study is to determine the influence of the mitochondrial open-reading-frame of the twelve S rRNA-c (MOTS-c) peptide on pancreatic cell physiology. Moreover, in this study, we examined the changes in MOTS-c secretion and expression under different conditions. Our experiments were conducted using laboratory cell line cultures, specifically the INS-1E and αTC-1 cell lines, which represent β and α pancreatic cells, respectively. As the pancreas is an endocrine organ, we also tested its hormone regulation capabilities. Furthermore, we assessed the secretion of MOTS-c after incubating the cells with glucose and free fatty acids. Additionally, we examined key cell culture parameters such as cell viability, proliferation, and apoptosis. The results obtained from this study show that MOTS-c has a significant impact on the physiology of pancreatic cells. Specifically, it lowers insulin secretion and expression in INS-1E cells and enhances glucagon secretion and expression in αTC-1 cells. Furthermore, MOTS-c affects cell viability and apoptosis. Interestingly, insulin and glucagon affect the MOTS-c secretion as well as glucose and free fatty acids. These experiments clearly show that MOTS-c is an important regulator of pancreatic metabolism, and there are numerous properties of MOTS-c yet to be discovered.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study7

6 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    In INS-1E beta-cell line cells, MOTS‑c lowered insulin secretion.

    In plain wordsIn cells, MOTS‑c lowered insulin release from INS-1E beta cells.

    Peptide
    MOTS-c
    Outcome
    DecreaseInsulin secretion in INS-1E cells
    Study

    n: not reported in source

    INS-1E cells
    Outcome group
    Glycemic control · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Specifically, it lowers insulin secretion and expression in INS-1E cells
    1 context sentence
    Summary · context
    Our experiments were conducted using laboratory cell line cultures, specifically the INS-1E and αTC-1 cell lines, which represent β and α pancreatic cells, respectively.
  2. 02LabIn vitro (cells)Efficacy outcome

    In INS-1E beta-cell line cells, MOTS‑c lowered insulin expression.

    In plain wordsIn cells, MOTS‑c lowered insulin production in INS-1E beta cells.

    Peptide
    MOTS-c
    Outcome
    DecreaseInsulin expression in INS-1E cells
    Study

    n: not reported in source

    INS-1E cells
    Outcome group
    Glycemic control · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Specifically, it lowers insulin secretion and expression in INS-1E cells
    1 context sentence
    Summary · context
    Our experiments were conducted using laboratory cell line cultures, specifically the INS-1E and αTC-1 cell lines, which represent β and α pancreatic cells, respectively.
  3. 03LabIn vitro (cells)Efficacy outcome

    In αTC-1 alpha-cell line cells, MOTS‑c enhanced glucagon secretion.

    In plain wordsIn cells, MOTS‑c raised glucagon release from αTC-1 alpha cells.

    Peptide
    MOTS-c
    Outcome
    IncreaseGlucagon secretion in αTC-1 cells
    Study

    n: not reported in source

    αTC-1 cells
    Outcome group
    Glycemic control · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    enhances glucagon secretion and expression in αTC-1 cells.
    1 context sentence
    Summary · context
    Our experiments were conducted using laboratory cell line cultures, specifically the INS-1E and αTC-1 cell lines, which represent β and α pancreatic cells, respectively.
  4. 04LabIn vitro (cells)Efficacy outcome

    In αTC-1 alpha-cell line cells, MOTS‑c enhanced glucagon expression.

    In plain wordsIn cells, MOTS‑c raised glucagon production in αTC-1 alpha cells.

    Peptide
    MOTS-c
    Outcome
    IncreaseGlucagon expression in αTC-1 cells
    Study

    n: not reported in source

    αTC-1 cells
    Outcome group
    Glycemic control · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    enhances glucagon secretion and expression in αTC-1 cells.
    1 context sentence
    Summary · context
    Our experiments were conducted using laboratory cell line cultures, specifically the INS-1E and αTC-1 cell lines, which represent β and α pancreatic cells, respectively.
  5. 05LabIn vitro (cells)Efficacy outcome

    In INS-1E and αTC-1 pancreatic cell lines, MOTS‑c affected cell viability; the direction is not stated.

    In plain wordsIn cells, MOTS‑c changed the survival of pancreatic cells; the abstract does not say in which direction.

    Peptide
    MOTS-c
    Outcome
    Direction not reportedCell viability of pancreatic cell lines
    Study

    n: not reported in source

    INS-1E and αTC-1 cells
    Outcome group
    Glycemic control · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Furthermore, MOTS-c affects cell viability and apoptosis.
    1 context sentence
    Summary · context
    Our experiments were conducted using laboratory cell line cultures, specifically the INS-1E and αTC-1 cell lines, which represent β and α pancreatic cells, respectively.
  6. 06LabIn vitro (cells)Efficacy outcome

    In INS-1E and αTC-1 pancreatic cell lines, MOTS‑c affected apoptosis; the direction is not stated.

    In plain wordsIn cells, MOTS‑c changed programmed cell death in pancreatic cells; the abstract does not say in which direction.

    Peptide
    MOTS-c
    Outcome
    Direction not reportedApoptosis of pancreatic cell lines
    Study

    n: not reported in source

    INS-1E and αTC-1 cells
    Outcome group
    Glycemic control · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Furthermore, MOTS-c affects cell viability and apoptosis.
    1 context sentence
    Summary · context
    Our experiments were conducted using laboratory cell line cultures, specifically the INS-1E and αTC-1 cell lines, which represent β and α pancreatic cells, respectively.

Natural levels measured1

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

  1. 07LabEndogenous levelIn vitro (cells)Mechanism

    In INS-1E and αTC-1 cells, insulin, glucagon, glucose and free fatty acids affected secretion of the cells' own MOTS‑c.

    In plain wordsIn cells, insulin, glucagon, glucose and fatty acids changed how much of the cells' own MOTS‑c was released.

    Peptide
    MOTS-c
    Outcome
    Direction not reportedMOTS-c secretion regulated by insulin, glucagon, glucose and free fatty acids
    Study

    n: not reported in source

    INS-1E and αTC-1 cells
    Outcome group
    Glycemic control · Endocrine and hormones

    Source sentences

    Summary
    Interestingly, insulin and glucagon affect the MOTS-c secretion as well as glucose and free fatty acids.
    1 context sentence
    Summary · context
    Our experiments were conducted using laboratory cell line cultures, specifically the INS-1E and αTC-1 cell lines, which represent β and α pancreatic cells, respectively.

    This result measures the body's own MOTS-c. Nobody was given the peptide. It is not evidence about taking it.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    9 Oct 2026, 18:17 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 02:47 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

PepBank is a research reference. Talk to a doctor before you use any peptide.