Liposome Encapsulation of the Palmitoyl-KTTKS Peptide: Structural and Functional Characterization.

Vitali A, Paolicelli P, Bigi B, Trilli J, Di Muzio L, Carriero VC, Casadei MA, Petralito S

Pharmaceutics2 Feb 2024Cited by 6 (Europe PMC)

PMID38399273PMCIDPMC10892597DOI10.3390/pharmaceutics16020219PepBankPUB-38399273

Summary

In this study, the amphiphilic N-palmitoyl-KTTKS peptide was integrated in the bilayer of egg-derived phosphatidylcholine (PC) vesicles using two different preparation methods, namely thin-film evaporation (TLE) and reverse-phase evaporation (REV). Both the REV and TLE methods allowed for the formation of homogeneous liposome dispersions (PdI < 0.20) with mean hydrodynamic diameters of <100 nm and <200 nm, respectively, a net negative surface charge and a percentage of structured phospholipids higher than 90%. The inclusion of the amphiphilic N-palmitoyl-KTTKS peptide within phospholipid-based vesicles could improve peptide stability and skin delivery. Therefore, the obtained liposomes were evaluated via experiments assessing the synthesis of collagen and the ECM in 3T3-NIH fibroblasts. The obtained results showed that, when delivered with PC liposomes, pal-KTTKS stimulated collagen production more than free pentapeptide and 1 mM ascorbic acid, used as a positive control.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study7

2 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Efficacy outcome
In 3T3-NIH fibroblasts, liposomal pal-KTTKS stimulated collagen production more than free pentapeptide.
In plain wordsIn cells, liposomal pal-KTTKS increased collagen production more than free pentapeptide.
Peptide
Matrixyl (palmitoyl pentapeptide-4)
Outcome
IncreaseCollagen production versus free peptide
Study
n: not reported in source
3T3-NIH fibroblasts
Comparator
Free pentapeptide
Outcome group
Skin and hair · Skin and wound
Studied dose
Dose not stated
Source sentences
Summary
The obtained results showed that, when delivered with PC liposomes, pal-KTTKS stimulated collagen production more than free pentapeptide and 1 mM ascorbic acid, used as a positive control.
1 context sentence(design, population, supporting numbers)
Summary · context
Therefore, the obtained liposomes were evaluated via experiments assessing the synthesis of collagen and the ECM in 3T3-NIH fibroblasts.
Show in the study text Report a problemFND-3839927304
02LabIn vitro (cells)Efficacy outcome
In 3T3-NIH fibroblasts, liposomal pal-KTTKS stimulated collagen production more than ascorbic acid.
In plain wordsIn cells, liposomal pal-KTTKS increased collagen production more than ascorbic acid.
Peptide
Matrixyl (palmitoyl pentapeptide-4)
Outcome
IncreaseCollagen production versus ascorbic acid
Study
n: not reported in source
3T3-NIH fibroblasts
Comparator
1 mM ascorbic acid
Outcome group
Skin and hair · Skin and wound
Studied dose
Dose not stated
Source sentences
Summary
The obtained results showed that, when delivered with PC liposomes, pal-KTTKS stimulated collagen production more than free pentapeptide and 1 mM ascorbic acid, used as a positive control.
1 context sentence(design, population, supporting numbers)
Summary · context
Therefore, the obtained liposomes were evaluated via experiments assessing the synthesis of collagen and the ECM in 3T3-NIH fibroblasts.
Show in the study text Report a problemFND-3839927305

Studies that did not test the peptide alone5

In this study, Matrixyl (palmitoyl pentapeptide-4) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Matrixyl (palmitoyl pentapeptide-4).

03LabNot a test of Matrixyl (palmitoyl pentapeptide-4) aloneIn vitro (cell-free)Activity measurement
In formulation tests, REV and TLE produced homogeneous liposome dispersions with polydispersity indices below 0.20.
In plain wordsIn lab tests, REV and TLE produced uniform liposome dispersions with polydispersity indices below 0.20.
Peptide
Matrixyl (palmitoyl pentapeptide-4)
Outcome
Not applicableLiposome polydispersity
Study
n: not reported in source
Outcome group
Formulation, delivery and stability · Pharmacology
Measurements
Polydispersity index
< 0.2 unitless
REV and TLE
Source sentences
Summary
Both the REV and TLE methods allowed for the formation of homogeneous liposome dispersions (PdI < 0.20) with mean hydrodynamic diameters of <100 nm and <200 nm, respectively, a net negative surface charge and a percentage of structured phospholipids higher than 90%.
In this study, Matrixyl (palmitoyl pentapeptide-4) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Matrixyl (palmitoyl pentapeptide-4).
Show in the study text Report a problemFND-3839927301
04LabNot a test of Matrixyl (palmitoyl pentapeptide-4) aloneIn vitro (cell-free)Activity measurement
In formulation tests, REV liposomes containing N-palmitoyl-KTTKS had mean hydrodynamic diameters below 100 nm.
In plain wordsIn lab tests, REV liposomes with N-palmitoyl-KTTKS had average diameters below 100 nm.
Peptide
Matrixyl (palmitoyl pentapeptide-4)
Outcome
Not applicableREV liposome hydrodynamic diameter
Study
n: not reported in source
Outcome group
Formulation, delivery and stability · Pharmacology
Measurements
Mean hydrodynamic diameter
< 100 nm
REV
Source sentences
Summary
Both the REV and TLE methods allowed for the formation of homogeneous liposome dispersions (PdI < 0.20) with mean hydrodynamic diameters of <100 nm and <200 nm, respectively, a net negative surface charge and a percentage of structured phospholipids higher than 90%.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, the amphiphilic N-palmitoyl-KTTKS peptide was integrated in the bilayer of egg-derived phosphatidylcholine (PC) vesicles using two different preparation methods, namely thin-film evaporation (TLE) and reverse-phase evaporation (REV).
In this study, Matrixyl (palmitoyl pentapeptide-4) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Matrixyl (palmitoyl pentapeptide-4).
Show in the study text Report a problemFND-3839927302
05LabNot a test of Matrixyl (palmitoyl pentapeptide-4) aloneIn vitro (cell-free)Activity measurement
In formulation tests, TLE liposomes containing N-palmitoyl-KTTKS had mean hydrodynamic diameters below 200 nm.
In plain wordsIn lab tests, TLE liposomes with N-palmitoyl-KTTKS had average diameters below 200 nm.
Peptide
Matrixyl (palmitoyl pentapeptide-4)
Outcome
Not applicableTLE liposome hydrodynamic diameter
Study
n: not reported in source
Outcome group
Formulation, delivery and stability · Pharmacology
Measurements
Mean hydrodynamic diameter
< 200 nm
TLE
Source sentences
Summary
Both the REV and TLE methods allowed for the formation of homogeneous liposome dispersions (PdI < 0.20) with mean hydrodynamic diameters of <100 nm and <200 nm, respectively, a net negative surface charge and a percentage of structured phospholipids higher than 90%.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, the amphiphilic N-palmitoyl-KTTKS peptide was integrated in the bilayer of egg-derived phosphatidylcholine (PC) vesicles using two different preparation methods, namely thin-film evaporation (TLE) and reverse-phase evaporation (REV).
In this study, Matrixyl (palmitoyl pentapeptide-4) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Matrixyl (palmitoyl pentapeptide-4).
Show in the study text Report a problemFND-3839927303
06LabNot a test of Matrixyl (palmitoyl pentapeptide-4) aloneIn vitro (cell-free)Activity measurement
In formulation tests, REV and TLE liposome dispersions had a net negative surface charge.
In plain wordsIn lab tests, REV and TLE liposome dispersions had a net negative surface charge.
Peptide
Matrixyl (palmitoyl pentapeptide-4)
Outcome
Not applicableLiposome surface charge
Study
n: not reported in source
Outcome group
Formulation, delivery and stability · Pharmacology
Source sentences
Summary
Both the REV and TLE methods allowed for the formation of homogeneous liposome dispersions (PdI < 0.20) with mean hydrodynamic diameters of <100 nm and <200 nm, respectively, a net negative surface charge and a percentage of structured phospholipids higher than 90%.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, the amphiphilic N-palmitoyl-KTTKS peptide was integrated in the bilayer of egg-derived phosphatidylcholine (PC) vesicles using two different preparation methods, namely thin-film evaporation (TLE) and reverse-phase evaporation (REV).
In this study, Matrixyl (palmitoyl pentapeptide-4) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Matrixyl (palmitoyl pentapeptide-4).
Show in the study text Report a problemFND-3839927306
07LabNot a test of Matrixyl (palmitoyl pentapeptide-4) aloneIn vitro (cell-free)Activity measurement
In formulation tests, REV and TLE liposome dispersions had more than 90% structured phospholipids.
In plain wordsIn lab tests, REV and TLE liposome dispersions had more than 90% structured phospholipids.
Peptide
Matrixyl (palmitoyl pentapeptide-4)
Outcome
Not applicableStructured phospholipids
Study
n: not reported in source
Outcome group
Formulation, delivery and stability · Pharmacology
Measurements
Structured phospholipids
> 90%
REV and TLE
Source sentences
Summary
Both the REV and TLE methods allowed for the formation of homogeneous liposome dispersions (PdI < 0.20) with mean hydrodynamic diameters of <100 nm and <200 nm, respectively, a net negative surface charge and a percentage of structured phospholipids higher than 90%.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, the amphiphilic N-palmitoyl-KTTKS peptide was integrated in the bilayer of egg-derived phosphatidylcholine (PC) vesicles using two different preparation methods, namely thin-film evaporation (TLE) and reverse-phase evaporation (REV).
In this study, Matrixyl (palmitoyl pentapeptide-4) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Matrixyl (palmitoyl pentapeptide-4).
Show in the study text Report a problemFND-3839927307

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
22 Oct 2026, 02:26 UTC
Study results updated
Version 1
21 Oct 2026, 18:59 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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Keywords

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