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Study · In vitro (cells) · 2023

4 results

Effect of Peptides on the Synthesis, Properties and Wound Healing Capacity of Silver Nanoparticles.

Papaioannou A, Liakopoulou A, Papoulis D, Gianni E, Gkolfi P, Zygouri E, Letsiou S, Hatziantoniou S

Pharmaceutics16 Oct 2023Cited by 9 (Europe PMC)

PMID37896231 (opens in a new tab)PMCIDPMC10609782 (opens in a new tab)DOI10.3390/pharmaceutics15102471 (opens in a new tab)PepBankPUB-37896231
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Summary

The aim of this study is the synthesis of novel peptide-silver nanoparticle conjugates with enhanced wound healing capacity. Peptide-silver nanoparticle conjugates were synthesized using myristoyl tetrapeptide 6 (MT6) or copper tripeptide 1 (CuTP1). Peptide-free silver nanoparticles (AgNP) were synthesized using NaBH4 and sodium citrate and were used as control. The addition of the peptides during or after the synthesis of nanoparticles and its impact on the properties of the synthesized peptide-silver nanoparticle conjugates were assessed. The monitoring of the synthesis of nanoparticles was achieved using ultraviolet-visible spectrophotometry (UV-/Vis). The characteristics and colloidal stability of the nanoparticles (size and ζ-potential distribution, morphology, composition and structure) were monitored using dynamic laser scattering (DLS), transmission electron microscopy (TEM), atomic absorption spectroscopy (AAS) and X-ray diffraction (XRD). The wound healing capacity of the peptide-silver nanoparticle conjugates was assessed using scratch test assay on fibroblasts (NIH/3T3). The results indicated that the addition of the peptides during the synthesis of nanoparticles lead to better yield of the reaction and more effective capping while the size distribution and ζ-potential of the conjugates indicated long-term colloidal stability. The MT6-AgNP conjugate exhibited 71.97 ± 4.35% wound closure, which was about 5.48-fold higher (p < 0.05) than the corresponding free MT6. The CuTP1-AgNP conjugate exhibited 62.37 ± 18.33% wound closure that was better by 2.82 fold (p < 0.05) compared to the corresponding free CuTP1. Both peptides led to the synthesis of silver nanoparticle conjugates with enhanced wound healing capacity compared to the respective free peptide or to the peptide-free AgNP (29.53 ± 4.71% wound closure, p < 0.05). Our findings demonstrated that the synthetized peptide-silver nanoparticle conjugates are promising ingredients for wound care formulation.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study4

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    In a fibroblast scratch assay, the CuTP1-silver nanoparticle conjugate gave 62.37% wound closure, 2.82 fold better than free CuTP1.

    In plain wordsIn cells, copper peptide attached to silver nanoparticles closed a scratch 2.82 fold better than free copper peptide.

    Peptide
    GHK-Cu
    Outcome
    IncreaseWound closure by CuTP1-silver nanoparticle conjugate versus free CuTP1
    Study

    n: not reported in source

    NIH/3T3 fibroblasts
    Comparator
    Free CuTP1
    Outcome group
    Wound healing · Skin and wound
    Studied dose
    Dose not stated

    Measurements

    Wound closure
    62.37%
    CuTP1-AgNP
    Fold improvement versus free CuTP1
    2.82 fold
    CuTP1-AgNP

    Source sentences

    Summary
    The CuTP1-AgNP conjugate exhibited 62.37 ± 18.33% wound closure that was better by 2.82 fold (p < 0.05) compared to the corresponding free CuTP1.
    1 context sentence
    Summary · context
    The wound healing capacity of the peptide-silver nanoparticle conjugates was assessed using scratch test assay on fibroblasts (NIH/3T3).
  2. 02LabIn vitro (cells)Efficacy outcome

    In a fibroblast scratch assay, the CuTP1-silver nanoparticle conjugate had enhanced wound closure compared with peptide-free silver nanoparticles (29.53% wound closure).

    In plain wordsIn cells, silver nanoparticles with copper peptide closed a scratch better than silver nanoparticles without peptide.

    Peptide
    GHK-Cu
    Outcome
    IncreaseWound closure by CuTP1-silver nanoparticle conjugate versus peptide-free AgNP
    Study

    n: not reported in source

    NIH/3T3 fibroblasts
    Comparator
    Peptide-free silver nanoparticles
    Outcome group
    Wound healing · Skin and wound
    Studied dose
    Dose not stated

    Measurements

    Wound closure
    29.53%
    peptide-free AgNP

    Source sentences

    Summary
    Both peptides led to the synthesis of silver nanoparticle conjugates with enhanced wound healing capacity compared to the respective free peptide or to the peptide-free AgNP (29.53 ± 4.71% wound closure, p < 0.05).
    1 context sentence
    Summary · context
    The wound healing capacity of the peptide-silver nanoparticle conjugates was assessed using scratch test assay on fibroblasts (NIH/3T3).

Studies that did not test the peptide alone2

In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHK-Cu.

  1. 03LabNot a test of GHK-Cu aloneIn vitro (cell-free)Other

    Adding the peptides during synthesis of silver nanoparticles gave a better reaction yield and more effective capping.

    In plain wordsIn lab tests, adding the peptides while making silver nanoparticles gave a better yield and better capping.

    Peptide
    GHK-Cu
    Outcome
    IncreaseYield and capping of silver nanoparticle synthesis
    Study

    n: not reported in source

    Outcome group
    Formulation, delivery and stability · Pharmacology

    Source sentences

    Summary
    The results indicated that the addition of the peptides during the synthesis of nanoparticles lead to better yield of the reaction and more effective capping
    1 context sentence
    Summary · context
    Peptide-silver nanoparticle conjugates were synthesized using myristoyl tetrapeptide 6 (MT6) or copper tripeptide 1 (CuTP1).

    In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK-Cu.

  2. 04LabNot a test of GHK-Cu aloneIn vitro (cell-free)Other

    The size distribution and ζ-potential of the conjugates indicated long-term colloidal stability.

    In plain wordsIn lab tests, the size and charge of the peptide-silver particles indicated they stay stable over a long time.

    Peptide
    GHK-Cu
    Outcome
    Not applicableColloidal stability of peptide-silver nanoparticle conjugates
    Study

    n: not reported in source

    Outcome group
    Formulation, delivery and stability · Pharmacology

    Source sentences

    Summary
    the size distribution and ζ-potential of the conjugates indicated long-term colloidal stability.
    1 context sentence
    Summary · context
    Peptide-silver nanoparticle conjugates were synthesized using myristoyl tetrapeptide 6 (MT6) or copper tripeptide 1 (CuTP1).

    In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK-Cu.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 4Current

    9 Oct 2026, 18:17 UTC

    Study results updated

  2. Versions 2 to 3

    9 Oct 2026, 03:03 UTC

    The same notice was added 2 times, first on 9 Oct 2026, 02:56 UTC. The status did not change between them.

    Study data updated · source update from Europe PMC

  3. Version 1

    9 Oct 2026, 02:56 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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