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Study · Animal study (in vivo) · mouse · 2023

10 results

Activation of AMPK ameliorates acute severe pancreatitis by suppressing pancreatic acinar cell necroptosis in obese mice models.

Wang K, Zhao A, Tayier D, Tan K, Song W, Cheng Q, Li X, Chen Z, Wei Q, Yuan Y, Yang Z

Cell death discovery30 Sept 2023Cited by 21 (Europe PMC)

PMID37777514 (opens in a new tab)PMCIDPMC10542799 (opens in a new tab)DOI10.1038/s41420-023-01655-z (opens in a new tab)PepBankPUB-37777514
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Summary

Obese people with acute pancreatitis (AP) have an increased risk of developing severe acute pancreatitis (SAP), which prolongs the length of hospital stay and increases mortality. Thus, elucidation of the mechanisms through which SAP occurs in obese individuals will provide clues for possible treatment targets. Differences in early events in obese or lean patients with AP have not been conclusively reported. We selected C57BL/6 mice as lean mice models, ob/ob mice or diet induced obese (DIO) mice as obese mice models and then induced experimental AP in mice via injections of caerulein. There were suppressed p-AMPK expressions in the pancreas of obese mice, compared with same-age lean C57BL/6 mice, which were further reduced in AP mice models. Obese AP mice were treated using AICAR, a direct AMPK agonist, which prevented pancreatic damage and cell death, suppressed pancreatic enzyme levels in serum, reduced the areas of fat saponification in the peritoneal cavity, prevented injury in other organs and decreased mice mortality rate. Further assays showed that AICAR activates p-AMPK to stabilize pro-caspase-8. Pro-caspase-8 enhances RIPK3 degradation, inhibits pancreatic acinar cell necroptosis, and downregulates the release of pancreatic enzymes. Thus, activation of AMPK by AICAR alleviates pancreatic acinar cell necroptosis and converts SAP to mild acute pancreatitis in obese mice.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study10

10 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In obese mice with acute pancreatitis, AICAR prevented pancreatic damage.

    In plain wordsIn obese mice with pancreatitis, AICAR prevented pancreatic damage.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreasePancreatic damage
    Study

    mousen: not reported in source

    Obese mice with caerulein-induced acute pancreatitis
    Outcome group
    Gastrointestinal protection · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Obese AP mice were treated using AICAR, a direct AMPK agonist, which prevented pancreatic damage and cell death, suppressed pancreatic enzyme levels in serum, reduced the areas of fat saponification in the peritoneal cavity, prevented injury in other organs and decreased mice mortality rate.
    1 context sentence
    Summary · context
    We selected C57BL/6 mice as lean mice models, ob/ob mice or diet induced obese (DIO) mice as obese mice models and then induced experimental AP in mice via injections of caerulein.
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In obese mice with acute pancreatitis, AICAR prevented cell death.

    In plain wordsIn obese mice with pancreatitis, AICAR prevented cell death.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreasePancreatic cell death
    Study

    mousen: not reported in source

    Obese mice with caerulein-induced acute pancreatitis
    Outcome group
    Gastrointestinal protection · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Obese AP mice were treated using AICAR, a direct AMPK agonist, which prevented pancreatic damage and cell death, suppressed pancreatic enzyme levels in serum, reduced the areas of fat saponification in the peritoneal cavity, prevented injury in other organs and decreased mice mortality rate.
    1 context sentence
    Summary · context
    We selected C57BL/6 mice as lean mice models, ob/ob mice or diet induced obese (DIO) mice as obese mice models and then induced experimental AP in mice via injections of caerulein.
  3. 03AnimalAnimal study (in vivo)Efficacy outcome

    In obese mice with acute pancreatitis, AICAR suppressed serum pancreatic enzyme levels.

    In plain wordsIn obese mice with pancreatitis, AICAR reduced pancreatic enzymes in blood.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseSerum pancreatic enzymes
    Study

    mousen: not reported in source

    Obese mice with caerulein-induced acute pancreatitis
    Outcome group
    Gastrointestinal protection · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Obese AP mice were treated using AICAR, a direct AMPK agonist, which prevented pancreatic damage and cell death, suppressed pancreatic enzyme levels in serum, reduced the areas of fat saponification in the peritoneal cavity, prevented injury in other organs and decreased mice mortality rate.
    1 context sentence
    Summary · context
    We selected C57BL/6 mice as lean mice models, ob/ob mice or diet induced obese (DIO) mice as obese mice models and then induced experimental AP in mice via injections of caerulein.
  4. 04AnimalAnimal study (in vivo)Efficacy outcome

    In obese mice with acute pancreatitis, AICAR reduced peritoneal fat saponification areas.

    In plain wordsIn obese mice with pancreatitis, AICAR reduced fat saponification in the abdominal cavity.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreasePeritoneal fat saponification
    Study

    mousen: not reported in source

    Obese mice with caerulein-induced acute pancreatitis
    Outcome group
    Gastrointestinal protection · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Obese AP mice were treated using AICAR, a direct AMPK agonist, which prevented pancreatic damage and cell death, suppressed pancreatic enzyme levels in serum, reduced the areas of fat saponification in the peritoneal cavity, prevented injury in other organs and decreased mice mortality rate.
    1 context sentence
    Summary · context
    We selected C57BL/6 mice as lean mice models, ob/ob mice or diet induced obese (DIO) mice as obese mice models and then induced experimental AP in mice via injections of caerulein.
  5. 05AnimalAnimal study (in vivo)Efficacy outcome

    In obese mice with acute pancreatitis, AICAR prevented injury in other organs.

    In plain wordsIn obese mice with pancreatitis, AICAR prevented injury in other organs.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseOther organ injury
    Study

    mousen: not reported in source

    Obese mice with caerulein-induced acute pancreatitis
    Outcome group
    Gastrointestinal protection · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Obese AP mice were treated using AICAR, a direct AMPK agonist, which prevented pancreatic damage and cell death, suppressed pancreatic enzyme levels in serum, reduced the areas of fat saponification in the peritoneal cavity, prevented injury in other organs and decreased mice mortality rate.
    1 context sentence
    Summary · context
    We selected C57BL/6 mice as lean mice models, ob/ob mice or diet induced obese (DIO) mice as obese mice models and then induced experimental AP in mice via injections of caerulein.
  6. 06AnimalAnimal study (in vivo)Safety outcome

    In obese mice with acute pancreatitis, AICAR decreased mortality.

    In plain wordsIn obese mice with pancreatitis, AICAR reduced deaths.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseMortality
    Study

    mousen: not reported in source

    Obese mice with caerulein-induced acute pancreatitis
    Outcome group
    Mortality · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Obese AP mice were treated using AICAR, a direct AMPK agonist, which prevented pancreatic damage and cell death, suppressed pancreatic enzyme levels in serum, reduced the areas of fat saponification in the peritoneal cavity, prevented injury in other organs and decreased mice mortality rate.
    1 context sentence
    Summary · context
    We selected C57BL/6 mice as lean mice models, ob/ob mice or diet induced obese (DIO) mice as obese mice models and then induced experimental AP in mice via injections of caerulein.
  7. 07AnimalAnimal study (in vivo)Mechanism

    In obese mice with pancreatitis, AICAR activated phosphorylated AMPK.

    In plain wordsIn obese mice with pancreatitis, AICAR activated phosphorylated AMPK.

    Peptide
    AICAR (acadesine)
    Outcome
    IncreaseAMPK phosphorylation
    Study

    mousen: not reported in source

    Obese mice with caerulein-induced acute pancreatitis
    Outcome group
    Receptor activity and mechanism · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Further assays showed that AICAR activates p-AMPK to stabilize pro-caspase-8.
    1 context sentence
    Summary · context
    We selected C57BL/6 mice as lean mice models, ob/ob mice or diet induced obese (DIO) mice as obese mice models and then induced experimental AP in mice via injections of caerulein.
  8. 08AnimalAnimal study (in vivo)Mechanism

    In obese mice with pancreatitis, AICAR stabilized pro-caspase-8 through AMPK activation.

    In plain wordsIn obese mice with pancreatitis, AICAR stabilized pro-caspase-8 through AMPK activation.

    Peptide
    AICAR (acadesine)
    Outcome
    IncreasePro-caspase-8 stability
    Study

    mousen: not reported in source

    Obese mice with caerulein-induced acute pancreatitis
    Outcome group
    Receptor activity and mechanism · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Further assays showed that AICAR activates p-AMPK to stabilize pro-caspase-8.
    1 context sentence
    Summary · context
    We selected C57BL/6 mice as lean mice models, ob/ob mice or diet induced obese (DIO) mice as obese mice models and then induced experimental AP in mice via injections of caerulein.
  9. 09AnimalAnimal study (in vivo)Mechanism

    In obese mice with pancreatitis, AICAR activated AMPK to stabilize pro-caspase-8, which enhanced RIPK3 degradation.

    In plain wordsIn obese mice with pancreatitis, AICAR stabilized pro-caspase-8 through AMPK activation, promoting RIPK3 breakdown.

    Peptide
    AICAR (acadesine)
    Outcome
    IncreaseRIPK3 degradation
    Study

    mousen: not reported in source

    Obese mice with caerulein-induced acute pancreatitis
    Outcome group
    Receptor activity and mechanism · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Further assays showed that AICAR activates p-AMPK to stabilize pro-caspase-8.
    2 context sentences
    Summary · context
    Pro-caspase-8 enhances RIPK3 degradation, inhibits pancreatic acinar cell necroptosis, and downregulates the release of pancreatic enzymes.
    Summary · context
    We selected C57BL/6 mice as lean mice models, ob/ob mice or diet induced obese (DIO) mice as obese mice models and then induced experimental AP in mice via injections of caerulein.
  10. 10AnimalAnimal study (in vivo)Mechanism

    In obese mice with pancreatitis, AICAR-mediated AMPK activation suppressed pancreatic acinar cell necroptosis through pro-caspase-8 stabilization.

    In plain wordsIn obese mice with pancreatitis, AICAR reduced a specific form of pancreatic cell death called necroptosis.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreasePancreatic acinar cell necroptosis
    Study

    mousen: not reported in source

    Obese mice with caerulein-induced acute pancreatitis
    Outcome group
    Gastrointestinal protection · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Pro-caspase-8 enhances RIPK3 degradation, inhibits pancreatic acinar cell necroptosis, and downregulates the release of pancreatic enzymes.
    3 context sentences
    Summary · context
    Further assays showed that AICAR activates p-AMPK to stabilize pro-caspase-8.
    Summary · context
    Thus, activation of AMPK by AICAR alleviates pancreatic acinar cell necroptosis and converts SAP to mild acute pancreatitis in obese mice.
    Summary · context
    We selected C57BL/6 mice as lean mice models, ob/ob mice or diet induced obese (DIO) mice as obese mice models and then induced experimental AP in mice via injections of caerulein.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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