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Study · Animal study (in vivo) · mouse · 2023

8 results

The Effect of Cerebrolysin in an Animal Model of Forebrain Ischemic-Reperfusion Injury: New Insights into the Activation of the Keap1/Nrf2/Antioxidant Signaling Pathway.

Marghani BH, Rezk S, Ateya AI, Alotaibi BS, Othman BH, Sayed SM, Alshehri MA, Shukry M, Mansour MM

International journal of molecular sciences28 Jul 2023Cited by 8 (Europe PMC)

PMID37569457 (opens in a new tab)PMCIDPMC10418386 (opens in a new tab)DOI10.3390/ijms241512080 (opens in a new tab)PepBankPUB-37569457
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Summary

Forebrain ischemia-reperfusion (IR) injury causes neurological impairments due to decreased cerebral autoregulation, hypoperfusion, and edema in the hours to days following the restoration of spontaneous circulation. This study aimed to examine the protective and/or therapeutic effects of cerebrolysin (CBL) in managing forebrain IR injury and any probable underlying mechanisms. To study the contribution of reperfusion to forebrain injury, we developed a transient dual carotid artery ligation (tDCAL/IR) mouse model. Five equal groups of six BLC57 mice were created: Group 1: control group (no surgery was performed); Group 2: sham surgery (surgery was performed without IR); Group 3: tDCAL/IR (surgery with IR via permanently ligating the left CA and temporarily closing the right CA for 30 min, followed by reperfusion for 72 h); Group 4: CBL + tDCAL/IR (CBL was given intravenously at a 60 mg/kg BW dose 30 min before IR); and Group 5: tDCAL/IR + CBL (CBL was administered i.v. at 60 mg/kg BW three hours after IR). At 72 h following IR, the mice were euthanized. CBL administration 3 h after IR improved neurological functional recovery, enhanced anti-inflammatory and antioxidant activities, alleviated apoptotic neuronal death, and inhibited reactive microglial and astrocyte activation, resulting in neuroprotection after IR injury in the tDCAL/IR + CBL mice group as compared to the other groups. Furthermore, CBL reduced the TLRs/NF-kB/cytokines while activating the Keap1/Nrf2/antioxidant signaling pathway. These results indicate that CBL may improve neurologic function in mice following IR.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study8

8 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In mice, Cerebrolysin given 3 h after ischemia/reperfusion improved neurological recovery compared with the other groups.

    In plain wordsIn mice, Cerebrolysin after ischemia/reperfusion improved neurological recovery compared with the other groups.

    Peptide
    Cerebrolysin
    Outcome
    IncreaseNeurological functional recovery
    Study

    mousen = 30

    transient dual carotid artery ligation ischemia/reperfusion
    Comparator
    Other experimental groups
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    60 mg/kg BW · intravenousAs given in this study (mouse).

    Source sentences

    Summary
    CBL administration 3 h after IR improved neurological functional recovery, enhanced anti-inflammatory and antioxidant activities, alleviated apoptotic neuronal death, and inhibited reactive microglial and astrocyte activation
    2 context sentences
    Summary · context
    resulting in neuroprotection after IR injury in the tDCAL/IR + CBL mice group as compared to the other groups.
    Summary · context
    To study the contribution of reperfusion to forebrain injury, we developed a transient dual carotid artery ligation (tDCAL/IR) mouse model.
  2. 02AnimalAnimal study (in vivo)Mechanism

    In mice, Cerebrolysin given 3 h after ischemia/reperfusion enhanced anti-inflammatory activity compared with the other groups.

    In plain wordsIn mice, Cerebrolysin after ischemia/reperfusion enhanced anti-inflammatory activity compared with the other groups.

    Peptide
    Cerebrolysin
    Outcome
    IncreaseAnti-inflammatory activity
    Study

    mousen = 30

    transient dual carotid artery ligation ischemia/reperfusion
    Comparator
    Other experimental groups
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    60 mg/kg BW · intravenousAs given in this study (mouse).

    Source sentences

    Summary
    CBL administration 3 h after IR improved neurological functional recovery, enhanced anti-inflammatory and antioxidant activities, alleviated apoptotic neuronal death, and inhibited reactive microglial and astrocyte activation
    2 context sentences
    Summary · context
    resulting in neuroprotection after IR injury in the tDCAL/IR + CBL mice group as compared to the other groups.
    Summary · context
    To study the contribution of reperfusion to forebrain injury, we developed a transient dual carotid artery ligation (tDCAL/IR) mouse model.
  3. 03AnimalAnimal study (in vivo)Mechanism

    In mice, Cerebrolysin given 3 h after ischemia/reperfusion enhanced antioxidant activity compared with the other groups.

    In plain wordsIn mice, Cerebrolysin after ischemia/reperfusion enhanced antioxidant activity compared with the other groups.

    Peptide
    Cerebrolysin
    Outcome
    IncreaseAntioxidant activity
    Study

    mousen = 30

    transient dual carotid artery ligation ischemia/reperfusion
    Comparator
    Other experimental groups
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    60 mg/kg BW · intravenousAs given in this study (mouse).

    Source sentences

    Summary
    CBL administration 3 h after IR improved neurological functional recovery, enhanced anti-inflammatory and antioxidant activities, alleviated apoptotic neuronal death, and inhibited reactive microglial and astrocyte activation
    2 context sentences
    Summary · context
    resulting in neuroprotection after IR injury in the tDCAL/IR + CBL mice group as compared to the other groups.
    Summary · context
    To study the contribution of reperfusion to forebrain injury, we developed a transient dual carotid artery ligation (tDCAL/IR) mouse model.
  4. 04AnimalAnimal study (in vivo)Efficacy outcome

    In mice, Cerebrolysin given 3 h after ischemia/reperfusion reduced apoptotic neuronal death compared with the other groups.

    In plain wordsIn mice, Cerebrolysin after ischemia/reperfusion reduced nerve cell death compared with the other groups.

    Peptide
    Cerebrolysin
    Outcome
    DecreaseApoptotic neuronal death
    Study

    mousen = 30

    transient dual carotid artery ligation ischemia/reperfusion
    Comparator
    Other experimental groups
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    60 mg/kg BW · intravenousAs given in this study (mouse).

    Source sentences

    Summary
    CBL administration 3 h after IR improved neurological functional recovery, enhanced anti-inflammatory and antioxidant activities, alleviated apoptotic neuronal death, and inhibited reactive microglial and astrocyte activation
    2 context sentences
    Summary · context
    resulting in neuroprotection after IR injury in the tDCAL/IR + CBL mice group as compared to the other groups.
    Summary · context
    To study the contribution of reperfusion to forebrain injury, we developed a transient dual carotid artery ligation (tDCAL/IR) mouse model.
  5. 05AnimalAnimal study (in vivo)Mechanism

    In mice, Cerebrolysin given 3 h after ischemia/reperfusion inhibited reactive microglial activation compared with the other groups.

    In plain wordsIn mice, Cerebrolysin after ischemia/reperfusion reduced microglial activation compared with the other groups.

    Peptide
    Cerebrolysin
    Outcome
    DecreaseReactive microglial activation
    Study

    mousen = 30

    transient dual carotid artery ligation ischemia/reperfusion
    Comparator
    Other experimental groups
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    60 mg/kg BW · intravenousAs given in this study (mouse).

    Source sentences

    Summary
    CBL administration 3 h after IR improved neurological functional recovery, enhanced anti-inflammatory and antioxidant activities, alleviated apoptotic neuronal death, and inhibited reactive microglial and astrocyte activation
    2 context sentences
    Summary · context
    resulting in neuroprotection after IR injury in the tDCAL/IR + CBL mice group as compared to the other groups.
    Summary · context
    To study the contribution of reperfusion to forebrain injury, we developed a transient dual carotid artery ligation (tDCAL/IR) mouse model.
  6. 06AnimalAnimal study (in vivo)Mechanism

    In mice, Cerebrolysin given 3 h after ischemia/reperfusion inhibited reactive astrocyte activation compared with the other groups.

    In plain wordsIn mice, Cerebrolysin after ischemia/reperfusion reduced astrocyte activation compared with the other groups.

    Peptide
    Cerebrolysin
    Outcome
    DecreaseReactive astrocyte activation
    Study

    mousen = 30

    transient dual carotid artery ligation ischemia/reperfusion
    Comparator
    Other experimental groups
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    60 mg/kg BW · intravenousAs given in this study (mouse).

    Source sentences

    Summary
    CBL administration 3 h after IR improved neurological functional recovery, enhanced anti-inflammatory and antioxidant activities, alleviated apoptotic neuronal death, and inhibited reactive microglial and astrocyte activation
    2 context sentences
    Summary · context
    resulting in neuroprotection after IR injury in the tDCAL/IR + CBL mice group as compared to the other groups.
    Summary · context
    To study the contribution of reperfusion to forebrain injury, we developed a transient dual carotid artery ligation (tDCAL/IR) mouse model.
  7. 07AnimalAnimal study (in vivo)Mechanism

    In mice with ischemia/reperfusion injury, Cerebrolysin reduced TLRs/NF-kB/cytokines.

    In plain wordsIn mice with ischemia/reperfusion injury, Cerebrolysin reduced TLRs/NF-kB/cytokines.

    Peptide
    Cerebrolysin
    Outcome
    DecreaseTLRs/NF-kB/cytokines
    Study

    mousen: not reported in source

    transient dual carotid artery ligation ischemia/reperfusion
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    60 mg/kg BW · intravenousAs given in this study (mouse).

    Source sentences

    Summary
    Furthermore, CBL reduced the TLRs/NF-kB/cytokines while activating the Keap1/Nrf2/antioxidant signaling pathway.
    1 context sentence
    Summary · context
    To study the contribution of reperfusion to forebrain injury, we developed a transient dual carotid artery ligation (tDCAL/IR) mouse model.
  8. 08AnimalAnimal study (in vivo)Mechanism

    In mice with ischemia/reperfusion injury, Cerebrolysin activated the Keap1/Nrf2/antioxidant signaling pathway.

    In plain wordsIn mice with ischemia/reperfusion injury, Cerebrolysin activated the Keap1/Nrf2/antioxidant signaling pathway.

    Peptide
    Cerebrolysin
    Outcome
    IncreaseKeap1/Nrf2/antioxidant signaling
    Study

    mousen: not reported in source

    transient dual carotid artery ligation ischemia/reperfusion
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    60 mg/kg BW · intravenousAs given in this study (mouse).

    Source sentences

    Summary
    Furthermore, CBL reduced the TLRs/NF-kB/cytokines while activating the Keap1/Nrf2/antioxidant signaling pathway.
    1 context sentence
    Summary · context
    To study the contribution of reperfusion to forebrain injury, we developed a transient dual carotid artery ligation (tDCAL/IR) mouse model.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    14 Oct 2026, 05:02 UTC

    Study results updated

  2. Version 1

    14 Oct 2026, 01:46 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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