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Study · Not a test of the peptide alone · 2023

5 results

Improved laccase production by Trametes versicolor using Copper-Glycyl-L-Histidyl-L-Lysine as a novel and high-efficient inducer.

Wang F, Yu X, Yu Z, Cui Y, Xu L, Huo S, Ding Z, Zhao L, Du L, Qiu Y

Frontiers in bioengineering and biotechnology25 Apr 2023Cited by 5 (Europe PMC)

PMID37180036 (opens in a new tab)PMCIDPMC10167017 (opens in a new tab)DOI10.3389/fbioe.2023.1176352 (opens in a new tab)PepBankPUB-37180036
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Summary

A highly efficient strategy using Copper-Glycyl-L-Histidyl-L-Lysine (GHK-Cu) as a novel inducer was developed to enhance laccase production by Trametes versicolor. After medium optimization, laccase activity increased by 12.77-fold compared to that without GHK-Cu. The laccase production of 1113.8 U L-1 was obtained by scaling-up culture in 5-L stirring tank. The laccase production induced by CuSO4 was poorer than that of GHK-Cu at the same mole concentration. GHK-Cu could increase the permeability of cell membrane with less damage, and it facilitated the adsorption, accumulation, and utilization of copper by fungal cells, which was beneficial for laccase synthesis. GHK-Cu induced better expression of laccase related genes than that of CuSO4, resulting in higher laccase production. This study provided a useful method for induced production of laccase by applying GHK chelated metal ion as a non-toxic inducer, which reduced the safety risk of laccase broth and provided the potential application of crude laccase in food industry. In addition, GHK can be used as the carrier of different metal ions to enhance the production of other metalloenzymes.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study5

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone5

In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHK-Cu.

  1. 01LabNot a test of GHK-Cu aloneIn vitro (cells)Activity measurement

    In Trametes versicolor cultures, laccase activity increased 12.77-fold with GHK‑Cu after medium optimization compared with no GHK‑Cu.

    In plain wordsIn lab tests with a fungus, GHK‑Cu raised laccase enzyme activity 12.77-fold compared with no GHK‑Cu.

    Peptide
    GHK-Cu
    Outcome
    IncreaseLaccase activity after medium optimization
    Study

    Trametes versicolorn: not reported in source

    Fungal culture
    Comparator
    Without GHK-Cu
    Outcome group
    Non-clinical applications · Pharmacology

    Measurements

    Laccase activity fold change
    12.77 fold
    after medium optimization, vs no GHK-Cu

    Source sentences

    Summary
    After medium optimization, laccase activity increased by 12.77-fold compared to that without GHK-Cu.
    1 context sentence
    Summary · context
    A highly efficient strategy using Copper-Glycyl-L-Histidyl-L-Lysine (GHK-Cu) as a novel inducer was developed to enhance laccase production by Trametes versicolor.

    In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK-Cu.

  2. 02LabNot a test of GHK-Cu aloneIn vitro (cells)Activity measurement

    Scaled-up culture in a 5-L stirring tank with GHK‑Cu gave a laccase production of 1113.8 U/L.

    In plain wordsIn lab tests with a fungus, scaled-up culture with GHK‑Cu gave 1113.8 U/L of laccase.

    Peptide
    GHK-Cu
    Outcome
    Not applicableLaccase production in 5-L stirring tank
    Study

    Trametes versicolorn: not reported in source

    Fungal culture
    Outcome group
    Non-clinical applications · Pharmacology

    Measurements

    Laccase production
    1113.8 U/L
    5-L stirring tank

    Source sentences

    Summary
    The laccase production of 1113.8 U L-1 was obtained by scaling-up culture in 5-L stirring tank.
    1 context sentence
    Summary · context
    A highly efficient strategy using Copper-Glycyl-L-Histidyl-L-Lysine (GHK-Cu) as a novel inducer was developed to enhance laccase production by Trametes versicolor.

    In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK-Cu.

  3. 03LabNot a test of GHK-Cu aloneIn vitro (cells)Activity measurement

    Laccase production was higher with GHK‑Cu than with CuSO4 at the same mole concentration.

    In plain wordsIn lab tests with a fungus, GHK‑Cu gave more laccase than copper sulfate at the same mole concentration.

    Peptide
    GHK-Cu
    Outcome
    IncreaseLaccase production versus CuSO4
    Study

    Trametes versicolorn: not reported in source

    Fungal culture
    Comparator
    CuSO4 at the same mole concentration
    Outcome group
    Non-clinical applications · Pharmacology

    Source sentences

    Summary
    The laccase production induced by CuSO4 was poorer than that of GHK-Cu at the same mole concentration.
    1 context sentence
    Summary · context
    A highly efficient strategy using Copper-Glycyl-L-Histidyl-L-Lysine (GHK-Cu) as a novel inducer was developed to enhance laccase production by Trametes versicolor.

    In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK-Cu.

  4. 04LabNot a test of GHK-Cu aloneIn vitro (cells)Mechanism

    GHK‑Cu could increase the permeability of the fungal cell membrane with less damage.

    In plain wordsIn lab tests, GHK‑Cu could make fungal cell membranes more permeable with little damage.

    Peptide
    GHK-Cu
    Outcome
    IncreaseCell membrane permeability
    Study

    Trametes versicolorn: not reported in source

    Fungal culture
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    GHK-Cu could increase the permeability of cell membrane with less damage
    1 context sentence
    Summary · context
    A highly efficient strategy using Copper-Glycyl-L-Histidyl-L-Lysine (GHK-Cu) as a novel inducer was developed to enhance laccase production by Trametes versicolor.

    In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK-Cu.

  5. 05LabNot a test of GHK-Cu aloneIn vitro (cells)Mechanism

    GHK‑Cu induced better expression of laccase related genes than CuSO4, resulting in higher laccase production.

    In plain wordsIn lab tests with a fungus, GHK‑Cu switched on laccase genes more than copper sulfate did.

    Peptide
    GHK-Cu
    Outcome
    IncreaseExpression of laccase related genes
    Study

    Trametes versicolorn: not reported in source

    Fungal culture
    Comparator
    CuSO4
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    GHK-Cu induced better expression of laccase related genes than that of CuSO4, resulting in higher laccase production.
    1 context sentence
    Summary · context
    A highly efficient strategy using Copper-Glycyl-L-Histidyl-L-Lysine (GHK-Cu) as a novel inducer was developed to enhance laccase production by Trametes versicolor.

    In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK-Cu.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    9 Oct 2026, 18:17 UTC

    Study results updated

  2. Version 2

    9 Oct 2026, 03:03 UTC

    Study data updated · source update from Europe PMC

  3. Version 1

    9 Oct 2026, 02:56 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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