Evaluation of GHK peptide-heparin interactions in multifunctional liposomal covering.

Nikolaeva V, Kamalov M, Abdullin TI, Salakhieva D, Chasov V, Rogov A, Zoughaib M

Journal of liposome research5 May 2023Cited by 3 (Europe PMC)

PMID37144381DOI10.1080/08982104.2023.2206894PepBankPUB-37144381

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study10

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone10

In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

01LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cells)Activity measurement
In 3T3 fibroblasts, heparin increased GHK-modified liposome accumulation compared with liposomes without heparin.
In plain wordsIn cells, heparin increased GHK-modified liposome accumulation compared with liposomes without heparin.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
IncreaseCellular accumulation
Study
n: not reported in source
3T3 fibroblasts
Comparator
GHK-modified liposomes without heparin
Outcome group
Formulation, delivery and stability · Pharmacology
Source sentences
Summary
The heparin component significantly promoted the accumulation of GHK-modified liposomes in 3T3 fibroblasts so that the composite liposomes exhibited the highest cell-penetrating activity.
1 context sentence(design, population, supporting numbers)
Summary · context
In situ association of membranotropic GHK derivative with unilamellar liposomes was performed to prepare GHK-modified liposomes with defined properties.
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-3714438105
02LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cells)Activity measurement
In 3T3 fibroblasts, GHK-heparin composite liposomes showed the highest cell-penetrating activity.
In plain wordsIn cells, GHK-heparin composite liposomes showed the highest cell penetration among the tested formulations.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
IncreaseCell penetration
Study
n: not reported in source
3T3 fibroblasts
Comparator
Other tested liposomal formulations
Outcome group
Formulation, delivery and stability · Pharmacology
Source sentences
Summary
The heparin component significantly promoted the accumulation of GHK-modified liposomes in 3T3 fibroblasts so that the composite liposomes exhibited the highest cell-penetrating activity.
1 context sentence(design, population, supporting numbers)
Summary · context
In situ association of membranotropic GHK derivative with unilamellar liposomes was performed to prepare GHK-modified liposomes with defined properties.
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-3714438106
03LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cells)Efficacy outcome
In fibroblast tests, the GHK-heparin composite formulation stimulated cell proliferation.
In plain wordsIn cells, the GHK-heparin composite formulation stimulated cell proliferation.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
IncreaseCell proliferation
Study
n: not reported in source
3T3 fibroblasts
Outcome group
Cell proliferation and differentiation · Pharmacology
Source sentences
Summary
Furthermore, the latter formulation stimulated cell proliferation and strongly inhibited ROS production and GSH depletion under oxidative stress conditions.
1 context sentence(design, population, supporting numbers)
Summary · context
In situ association of membranotropic GHK derivative with unilamellar liposomes was performed to prepare GHK-modified liposomes with defined properties.
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-3714438107
04LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cells)Efficacy outcome
In fibroblasts under oxidative stress, the GHK-heparin composite formulation strongly inhibited ROS production.
In plain wordsIn cells under oxidative stress, the GHK-heparin composite formulation inhibited ROS production.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
DecreaseROS production
Study
n: not reported in source
3T3 fibroblasts under oxidative stress
Outcome group
Oxidative stress · Pharmacology
Source sentences
Summary
Furthermore, the latter formulation stimulated cell proliferation and strongly inhibited ROS production and GSH depletion under oxidative stress conditions.
1 context sentence(design, population, supporting numbers)
Summary · context
In situ association of membranotropic GHK derivative with unilamellar liposomes was performed to prepare GHK-modified liposomes with defined properties.
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-3714438108
05LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cells)Efficacy outcome
In fibroblasts under oxidative stress, the GHK-heparin composite formulation strongly inhibited GSH depletion.
In plain wordsIn cells under oxidative stress, the GHK-heparin composite formulation inhibited GSH depletion.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
DecreaseGSH depletion
Study
n: not reported in source
3T3 fibroblasts under oxidative stress
Outcome group
Oxidative stress · Pharmacology
Source sentences
Summary
Furthermore, the latter formulation stimulated cell proliferation and strongly inhibited ROS production and GSH depletion under oxidative stress conditions.
1 context sentence(design, population, supporting numbers)
Summary · context
In situ association of membranotropic GHK derivative with unilamellar liposomes was performed to prepare GHK-modified liposomes with defined properties.
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-3714438109
06LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cell-free)Activity measurement
In laboratory tests, surface GHK on liposomes interacted specifically with heparin compared with other polysaccharides and the RGD counterpart.
In plain wordsIn lab tests, surface GHK interacted specifically with heparin compared with other polysaccharides and the RGD counterpart.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
Not applicableHeparin interaction
Study
n: not reported in source
Comparator
Other polysaccharides and RGD counterpart
Outcome group
Formulation, delivery and stability · Pharmacology
Source sentences
Summary
According to DLS, the GHK component on the liposomal surface interacted with heparin in a specific manner compared to other polysaccharides and RGD counterpart, whereas ITC analysis of such interactions was complicated.
1 context sentence(design, population, supporting numbers)
Summary · context
In situ association of membranotropic GHK derivative with unilamellar liposomes was performed to prepare GHK-modified liposomes with defined properties.
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-3714438101
07LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cell-free)Other
In laboratory tests, GHK-heparin composite liposomes had low size dispersity.
In plain wordsIn lab tests, GHK-heparin composite liposomes had low size dispersity.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
Not applicableSize dispersity
Study
n: not reported in source
Outcome group
Formulation, delivery and stability · Pharmacology
Source sentences
Summary
The resulting composite liposomes possessed low size dispersity, increased anionic charge, and mechanical rigidity.
1 context sentence(design, population, supporting numbers)
Summary · context
In situ association of membranotropic GHK derivative with unilamellar liposomes was performed to prepare GHK-modified liposomes with defined properties.
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-3714438102
08LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cell-free)Other
In laboratory tests, GHK-heparin composite liposomes showed increased anionic charge.
In plain wordsIn lab tests, GHK-heparin composite liposomes showed increased anionic charge.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
IncreaseAnionic charge
Study
n: not reported in source
Outcome group
Formulation, delivery and stability · Pharmacology
Source sentences
Summary
The resulting composite liposomes possessed low size dispersity, increased anionic charge, and mechanical rigidity.
1 context sentence(design, population, supporting numbers)
Summary · context
In situ association of membranotropic GHK derivative with unilamellar liposomes was performed to prepare GHK-modified liposomes with defined properties.
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-3714438103
09LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cell-free)Other
In laboratory tests, GHK-heparin composite liposomes showed mechanical rigidity.
In plain wordsIn lab tests, GHK-heparin composite liposomes showed mechanical rigidity.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
Not applicableMechanical rigidity
Study
n: not reported in source
Outcome group
Formulation, delivery and stability · Pharmacology
Source sentences
Summary
The resulting composite liposomes possessed low size dispersity, increased anionic charge, and mechanical rigidity.
1 context sentence(design, population, supporting numbers)
Summary · context
In situ association of membranotropic GHK derivative with unilamellar liposomes was performed to prepare GHK-modified liposomes with defined properties.
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-3714438104
10LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cell-free)Other
In lab tests, ITC analysis of interactions between GHK-presenting liposomes and heparin was complicated.
In plain wordsIn lab tests, ITC analysis of interactions between GHK-presenting liposomes and heparin was complicated.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
Not applicableITC interaction analysis
Study
n: not reported in source
Outcome group
Formulation, delivery and stability · Pharmacology
Source sentences
Summary
According to DLS, the GHK component on the liposomal surface interacted with heparin in a specific manner compared to other polysaccharides and RGD counterpart, whereas ITC analysis of such interactions was complicated.
1 context sentence(design, population, supporting numbers)
Summary · context
In situ association of membranotropic GHK derivative with unilamellar liposomes was performed to prepare GHK-modified liposomes with defined properties.
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-3714438110

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
14 Oct 2026, 05:02 UTC
Study results updated
Version 1
14 Oct 2026, 01:46 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Journal Article

MeSH terms

Keywords

PepBank is a research reference. Talk to a doctor before you use any peptide.