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Study · Not a test of the peptide alone · 2022

5 results

What are oxytocin assays measuring? Epitope mapping, metabolites, and comparisons of wildtype & knockout mouse urine.

Gnanadesikan GE, Hammock EAD, Tecot SR, Lewis RJ, Hart R, Carter CS, MacLean EL

Psychoneuroendocrinology8 Jun 2022Cited by 23 (Europe PMC)

PMID35714438 (opens in a new tab)PMCIDPMC9807061 (opens in a new tab)DOI10.1016/j.psyneuen.2022.105827 (opens in a new tab)PepBankPUB-35714438
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone5

In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Oxytocin.

  1. 01LabNot a test of Oxytocin aloneEx vivo tissueOther

    In urine from oxytocin knockout and wildtype mice, unextracted samples gave considerable assay interference, with similar measured oxytocin distributions in both genotypes.

    In plain wordsIn mouse urine tests, other substances disturbed the oxytocin assay, and knockout mice without oxytocin still gave similar readings to normal mice.

    Peptide
    Oxytocin
    Outcome
    Not applicableImmunoassay interference in unextracted urine
    Study

    mousen: not reported in source

    Urine samples from oxytocin knockout and wildtype mice
    Outcome group
    Analytical methods and detection · Pharmacology

    Source sentences

    Summary
    Using samples from oxytocin knockout (KO) and wildtype (WT) mice, we find evidence of considerable interference in unextracted urine samples, with similar distributions of measured oxytocin in both genotypes.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

  2. 02LabNot a test of Oxytocin aloneEx vivo tissueOther

    In mouse urine, reversed-phase solid-phase extraction reduced but did not remove false-positive signal on three oxytocin immunoassay kits.

    In plain wordsIn lab tests on mouse urine, a common clean-up step did not remove false oxytocin signals on three assay kits.

    Peptide
    Oxytocin
    Outcome
    Not applicableReversed-phase solid-phase extraction and false-positive signal
    Study

    mousen: not reported in source

    Urine samples from oxytocin knockout and wildtype mice
    Outcome group
    Analytical methods and detection · Pharmacology

    Source sentences

    Summary
    Importantly, although this interference can be reduced by a reversed-phase solid-phase extraction (SPE), this common approach is not sufficient for eliminating false-positive signal on three immunoassay kits.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

  3. 03LabNot a test of Oxytocin aloneEx vivo tissueOther

    In mouse urine, a new mixed-mode cation-exchange extraction minimized interference, with knockout samples below the limit of detection, while retaining oxytocin from wildtype urine.

    In plain wordsIn lab tests on mouse urine, a new clean-up method removed assay interference and still kept the real oxytocin.

    Peptide
    Oxytocin
    Outcome
    Not applicableMixed-mode cation-exchange extraction and interference
    Study

    mousen: not reported in source

    Urine samples from oxytocin knockout and wildtype mice
    Outcome group
    Analytical methods and detection · Pharmacology

    Source sentences

    Summary
    We present a new mixed-mode cation-exchange SPE method that minimizes interference-with knockout samples measuring below the assay's limit of detection-while effectively retaining oxytocin from the urine of wildtype mice.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

  4. 04LabNot a test of Oxytocin aloneEx vivo tissueOther

    The new extraction method showed good parallelism and spike recovery for oxytocin in urine from mice, dogs, sifakas and humans.

    In plain wordsIn lab tests on urine samples from several species, the new method measured oxytocin well.

    Peptide
    Oxytocin
    Outcome
    Not applicableParallelism and spike recovery across species
    Study

    mice, dogs, sifakas, humansn: not reported in source

    Urine samples
    Outcome group
    Analytical methods and detection · Pharmacology

    Source sentences

    Summary
    This method demonstrates good parallelism and spike recovery across multiple species (mice, dogs, sifakas, humans).

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

  5. 05LabNot a test of Oxytocin aloneIn vitro (cell-free)Other

    In an epitope mapping test, the Arbor Assays antibody showed considerable cross-reactivity with three oxytocin fragments that share the core epitope, more with longer fragments.

    In plain wordsIn lab tests, the assay antibody also reacted with three oxytocin fragments, more strongly with longer fragments.

    Peptide
    Oxytocin
    Outcome
    Not applicableAntibody cross-reactivity with oxytocin fragments
    Study

    n: not reported in source

    Arbor Assays antibody epitope mapping
    Outcome group
    Analytical methods and detection · Pharmacology

    Measurements

    Cross-reactivity
    0.5 to 52%
    three oxytocin fragments, by molarity

    Source sentences

    Summary
    We found considerable cross-reactivity (0.5-52% by-molarity) for three fragments of oxytocin that share the core epitope, with more cross-reactivity for longer fragments.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    21 Oct 2026, 18:59 UTC

    Study data updated · source update from Europe PMC

  2. Version 2

    11 Oct 2026, 00:50 UTC

    Study results updated

  3. Version 1

    9 Oct 2026, 02:48 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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