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Study · Not a test of the peptide alone · 2022

4 results

Detection of insulin analogues and large peptides >2 kDa in urine.

Cox HD, Knussmann GN, Moore C, Eichner D

Drug testing and analysis13 Mar 2022Cited by 10 (Europe PMC)

PMID35261185 (opens in a new tab)DOI10.1002/dta.3249 (opens in a new tab)PepBankPUB-35261185
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone4

In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking MGF / PEG-MGF.

  1. 01LabNot a test of MGF / PEG-MGF aloneIn vitro (cell-free)Other

    In a urine LC-MS/MS assay, the limit of detection was between 5 and 50 pg/ml for the peptide classes other than insulin analogues, which include MGF analogues.

    In plain wordsIn lab tests, a urine assay detected large peptides such as MGF analogues at 5 to 50 pg/ml.

    Peptide
    MGF / PEG-MGF
    Outcome
    Not applicableLimit of detection for non-insulin peptide classes
    Study

    n: not reported in source

    Urine samples, LC-MS/MS assay
    Outcome group
    Pharmacokinetics · Pharmacology

    Measurements

    Limit of detection
    5 to 50 pg/ml
    peptide classes other than insulin analogues

    Source sentences

    Summary
    The limit of detection for insulin analogues is between 5 and 25 pg/ml and between 5 and 50 pg/ml for the other peptide classes.
    2 context sentences
    Summary · context
    In addition to insulin analogues, the large peptides include a structurally diverse set of peptides including analogues of growth hormone releasing hormone (GHRH), insulin-like growth factor-1 (IGF-1), and mechano-growth factor (MGF).
    Summary · context
    In this report, a high throughput method is described that allows sensitive detection of four classes of large peptides in one assay.

    In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MGF / PEG-MGF.

  2. 02LabNot a test of MGF / PEG-MGF aloneIn vitro (cell-free)Other

    In the urine LC-MS/MS assay, specificity was good, with no interfering peaks in blank urine samples.

    In plain wordsIn lab tests, the urine assay for large peptides showed no interfering signals in blank urine.

    Peptide
    MGF / PEG-MGF
    Outcome
    Not applicableAssay specificity
    Study

    n: not reported in source

    Urine samples, LC-MS/MS assay
    Outcome group
    Pharmacokinetics · Pharmacology

    Source sentences

    Summary
    Specificity was good with no detection of interfering peaks in blank urine samples.
    1 context sentence
    Summary · context
    In this report, a high throughput method is described that allows sensitive detection of four classes of large peptides in one assay.

    In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MGF / PEG-MGF.

  3. 03LabNot a test of MGF / PEG-MGF aloneIn vitro (cell-free)Other

    In the urine LC-MS/MS assay, carryover from a high concentration sample was not observed.

    In plain wordsIn lab tests, the urine assay for large peptides showed no carryover from a high concentration sample.

    Peptide
    MGF / PEG-MGF
    Outcome
    No significant changeAssay carryover
    Study

    n: not reported in source

    Urine samples, LC-MS/MS assay
    Outcome group
    Pharmacokinetics · Pharmacology

    Source sentences

    Summary
    Carryover from a high concentration sample was not observed and the post-extraction stability was between 77% and 107%.
    1 context sentence
    Summary · context
    In this report, a high throughput method is described that allows sensitive detection of four classes of large peptides in one assay.

    In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MGF / PEG-MGF.

  4. 04LabNot a test of MGF / PEG-MGF aloneIn vitro (cell-free)Other

    In the urine LC-MS/MS assay, post-extraction stability was between 77% and 107%.

    In plain wordsIn lab tests, extracted peptides in the urine assay stayed stable at 77% to 107%.

    Peptide
    MGF / PEG-MGF
    Outcome
    Not applicablePost-extraction stability
    Study

    n: not reported in source

    Urine samples, LC-MS/MS assay
    Outcome group
    Pharmacokinetics · Pharmacology

    Measurements

    Post-extraction stability
    77 to 107%
    urine assay

    Source sentences

    Summary
    the post-extraction stability was between 77% and 107%.
    1 context sentence
    Summary · context
    In this report, a high throughput method is described that allows sensitive detection of four classes of large peptides in one assay.

    In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MGF / PEG-MGF.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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