Specificity of plasma oxytocin immunoassays: A comparison of commercial assays and sample preparation techniques using oxytocin knockout and wildtype mice.

Gnanadesikan GE, Hammock EAD, Tecot SR, Carter CS, MacLean EL

Psychoneuroendocrinology26 Jul 2021Cited by 27 (Europe PMC)

PMID34364024PMCIDPMC8487999DOI10.1016/j.psyneuen.2021.105368PepBankPUB-34364024

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone5

In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Oxytocin.

01AnimalNot a test of Oxytocin aloneAnimal study (in vivo)Other
An Arbor Assays immunoassay clearly separated wildtype from oxytocin knockout mouse plasma, with and without extraction; knockout samples were below the detection limit.
In plain wordsIn mouse plasma, an oxytocin test kit clearly told normal mice from oxytocin knockout mice, with or without sample cleanup.
Peptide
Oxytocin
Outcome
Not applicableArbor Assays kit: genotype contrast, with and without extraction
Study
mousen: not reported in source
Plasma from wildtype and oxytocin knockout mice
Outcome group
Analytical methods and detection · Endocrine and hormones
Measurements
Plasma oxytocin, unextracted
468 pg/ml
wildtype mice
Plasma oxytocin, extracted
381 pg/ml
wildtype mice
Source sentences
Summary
Using a commercially available kit from Arbor Assays, we demonstrate that both techniques generate a clear contrast between genotypes, with wildtype samples containing high concentrations of oxytocin (unextracted mean = 468 pg/ml; extracted mean = 381 pg/ml), while knockout samples measured below th…
2 context sentences(design, population, supporting numbers)
Summary · context
Here, we assess the specificity of oxytocin immunoassays using plasma samples from wildtype (WT) and oxytocin knockout (KO) mice.
Summary · context
Plasma samples from both genotypes were measured using immunoassay and were measured with or without a solid-phase extraction.
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-3436402401
02AnimalNot a test of Oxytocin aloneAnimal study (in vivo)Other
Analytical validation showed good parallelism and spike recovery for both the extracted and unextracted methods.
In plain wordsIn lab tests, both sample methods passed standard accuracy checks for measuring oxytocin.
Peptide
Oxytocin
Outcome
Not applicableParallelism and spike recovery
Study
mousen: not reported in source
Plasma from wildtype and oxytocin knockout mice
Outcome group
Analytical methods and detection · Endocrine and hormones
Source sentences
Summary
Analytical validations demonstrated good parallelism and spike recovery for both methods.
2 context sentences(design, population, supporting numbers)
Summary · context
Here, we assess the specificity of oxytocin immunoassays using plasma samples from wildtype (WT) and oxytocin knockout (KO) mice.
Summary · context
Plasma samples from both genotypes were measured using immunoassay and were measured with or without a solid-phase extraction.
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-3436402402
03AnimalNot a test of Oxytocin aloneAnimal study (in vivo)Other
Wildtype samples measured with both procedures were highly correlated, although unextracted samples read significantly higher.
In plain wordsIn mouse plasma, the two sample methods gave closely matching oxytocin results, but the unextracted method gave higher readings.
Peptide
Oxytocin
Outcome
Not applicableCorrelation of extracted and unextracted samples
Study
mousen: not reported in source
Plasma from wildtype and oxytocin knockout mice
Outcome group
Analytical methods and detection · Endocrine and hormones
Source sentences
Summary
Furthermore, the same wildtype samples measured with both procedures were highly correlated (r = 0.95), although unextracted samples measured at significantly higher concentrations (p = 2.0 ×10^-7, Cohen's d = 2.65).
2 context sentences(design, population, supporting numbers)
Summary · context
Here, we assess the specificity of oxytocin immunoassays using plasma samples from wildtype (WT) and oxytocin knockout (KO) mice.
Summary · context
Plasma samples from both genotypes were measured using immunoassay and were measured with or without a solid-phase extraction.
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-3436402403
04AnimalNot a test of Oxytocin aloneAnimal study (in vivo)Other
The Cayman Chemical kit gave results similar to Arbor Assays, with a clean signal differentiating wildtype from knockout plasma with and without extraction.
In plain wordsIn mouse plasma, a second oxytocin test kit also told normal mice from knockout mice with a clean signal.
Peptide
Oxytocin
Outcome
Not applicableCayman Chemical kit performance
Study
mousen: not reported in source
Plasma from wildtype and oxytocin knockout mice
Outcome group
Analytical methods and detection · Endocrine and hormones
Source sentences
Summary
The Cayman Chemical kit produced results similar to Arbor Assays with a clean signal differentiating WT and KO plasma, both with and without an extraction step.
2 context sentences(design, population, supporting numbers)
Summary · context
Here, we assess the specificity of oxytocin immunoassays using plasma samples from wildtype (WT) and oxytocin knockout (KO) mice.
Summary · context
Plasma samples from both genotypes were measured using immunoassay and were measured with or without a solid-phase extraction.
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-3436402404
05AnimalNot a test of Oxytocin aloneAnimal study (in vivo)Other
The Enzo kit differentiated genotypes but, without extraction, was more susceptible to interference and gave a false positive signal in knockout plasma.
In plain wordsIn mouse plasma, a third oxytocin test kit gave false positive signals in knockout mice unless the samples were cleaned first.
Peptide
Oxytocin
Outcome
Not applicableEnzo kit interference without extraction
Study
mousen: not reported in source
Plasma from wildtype and oxytocin knockout mice
Outcome group
Analytical methods and detection · Endocrine and hormones
Source sentences
Summary
The Enzo kit also differentiated the genotypes, with correlation between extracted and unextracted samples, but was considerably more susceptible to interference without the extraction, as evidenced by false positive signal in KO plasma samples.
2 context sentences(design, population, supporting numbers)
Summary · context
Here, we assess the specificity of oxytocin immunoassays using plasma samples from wildtype (WT) and oxytocin knockout (KO) mice.
Summary · context
Plasma samples from both genotypes were measured using immunoassay and were measured with or without a solid-phase extraction.
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-3436402405

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
11 Oct 2026, 00:50 UTC
Study results updated
Version 1
9 Oct 2026, 02:48 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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