Development & validation of LC-MS/MS assay for 5-amino-1-methyl quinolinium in rat plasma: Application to pharmacokinetic and oral bioavailability studies.

Awosemo O, Neelakantan H, Watowich S, Ma J, Wu L, Chow DS, Liang D

Journal of pharmaceutical and biomedical analysis8 Jul 2021Cited by 7 (Europe PMC)

PMID34304009DOI10.1016/j.jpba.2021.114255PepBankPUB-34304009

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study16

6 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Pharmacokinetics
In rats, oral 5-AMQ produced a mean maximum plasma concentration of 2252 ng/mL.
In plain wordsIn rats, oral 5-AMQ produced a mean maximum plasma concentration of 2252 ng/mL.
Peptide
5-amino-1MQ
Outcome
Not applicableOral maximum plasma concentration
Study
ratn: not reported in source
rat pharmacokinetic study
Outcome group
Pharmacokinetics · Pharmacology
Studied dose
Dose not stated · oralAs given in this study (rat).
Measurements
maximum plasma concentration
2252 ng/mL
oral
Source sentences
Summary
5-AMQ displayed substantial plasma exposures via IV and oral route, with a mean maximum plasma concentration of 2252 ng/mL after oral administration
Report a problemFND-3430400911
02AnimalAnimal study (in vivo)Pharmacokinetics
In rats, intravenous 5-AMQ produced a mean AUC of 3708 h.ng/mL.
In plain wordsIn rats, intravenous 5-AMQ produced a mean AUC of 3708 h.ng/mL.
Peptide
5-amino-1MQ
Outcome
Not applicableIntravenous exposure AUC
Study
ratn: not reported in source
rat pharmacokinetic study
Outcome group
Pharmacokinetics · Pharmacology
Studied dose
Dose not stated · intravenousAs given in this study (rat).
Measurements
AUC
3708 h.ng/mL
IV
Source sentences
Summary
mean area under the curve (AUC_0-∞) of 3708 h.ng/mL and 14431 h.ng/mL for the IV and oral groups, respectively,
Report a problemFND-3430400912
03AnimalAnimal study (in vivo)Pharmacokinetics
In rats, oral 5-AMQ produced a mean AUC of 14431 h.ng/mL.
In plain wordsIn rats, oral 5-AMQ produced a mean AUC of 14431 h.ng/mL.
Peptide
5-amino-1MQ
Outcome
Not applicableOral exposure AUC
Study
ratn: not reported in source
rat pharmacokinetic study
Outcome group
Pharmacokinetics · Pharmacology
Studied dose
Dose not stated · oralAs given in this study (rat).
Measurements
AUC
14,431 h.ng/mL
oral
Source sentences
Summary
mean area under the curve (AUC_0-∞) of 3708 h.ng/mL and 14431 h.ng/mL for the IV and oral groups, respectively,
Report a problemFND-3430400913
04AnimalAnimal study (in vivo)Pharmacokinetics
In rats, intravenous 5-AMQ had a mean terminal elimination half-life of 3.80 ± 1.10 h.
In plain wordsIn rats, intravenous 5-AMQ had a mean terminal elimination half-life of 3.80 ± 1.10 h.
Peptide
5-amino-1MQ
Outcome
Not applicableIntravenous terminal elimination half-life
Study
ratn: not reported in source
rat pharmacokinetic study
Outcome group
Pharmacokinetics · Pharmacology
Studied dose
Dose not stated · intravenousAs given in this study (rat).
Measurements
terminal half-life
3.8 h
intravenous
Source sentences
Summary
mean terminal elimination half-life of 3.80 ± 1.10 h and 6.90 ± 1.20 h respectively after intravenous and oral dose
Report a problemFND-3430400914
05AnimalAnimal study (in vivo)Pharmacokinetics
In rats, oral 5-AMQ had a mean terminal elimination half-life of 6.90 ± 1.20 h.
In plain wordsIn rats, oral 5-AMQ had a mean terminal elimination half-life of 6.90 ± 1.20 h.
Peptide
5-amino-1MQ
Outcome
Not applicableOral terminal elimination half-life
Study
ratn: not reported in source
rat pharmacokinetic study
Outcome group
Pharmacokinetics · Pharmacology
Studied dose
Dose not stated · oralAs given in this study (rat).
Measurements
terminal half-life
6.9 h
oral
Source sentences
Summary
mean terminal elimination half-life of 3.80 ± 1.10 h and 6.90 ± 1.20 h respectively after intravenous and oral dose
Report a problemFND-3430400915
06AnimalAnimal study (in vivo)Pharmacokinetics
In rats, 5-AMQ oral bioavailability was 38.4 %.
In plain wordsIn rats, 5-AMQ oral bioavailability was 38.4 %.
Peptide
5-amino-1MQ
Outcome
Not applicableOral bioavailability
Study
ratn: not reported in source
rat pharmacokinetic study
Outcome group
Pharmacokinetics · Pharmacology
Studied dose
Dose not stated · oralAs given in this study (rat).
Measurements
oral bioavailability
38.4%
oral
Source sentences
Summary
and a good oral bioavailability (F % = 38.4).
1 context sentence(design, population, supporting numbers)
Summary · context
The validated, sensitive, and reproducible LC-MS/MS method for 5-AMQ in rat plasma and urine samples was effectively applied to a pharmacokinetic study in rats with IV and oral administration of 5-AMQ.
Report a problemFND-3430400916

Studies that did not test the peptide alone10

In this study, 5-amino-1MQ was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking 5-amino-1MQ.

07LabNot a test of 5-amino-1MQ aloneIn vitro (cell-free)Activity measurement
In lab tests, 5-AMQ calibration curves in rat plasma and urine were linear from 10-2500 ng/mL.
In plain wordsIn lab tests, 5-AMQ calibration curves in rat plasma and urine were linear from 10-2500 ng/mL.
Peptide
5-amino-1MQ
Outcome
Not applicableCalibration linearity
Study
ratn: not reported in source
rat plasma and urine assay
Outcome group
Pharmacokinetics · Pharmacology
Measurements
linear range
10 to 2500 ng/mL
rat plasma and urine
Source sentences
Summary
The standard curves of 5-AMQ in rat urine and plasma samples were linear in the concentration range of 10-2500 ng/mL.
In this study, 5-amino-1MQ was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking 5-amino-1MQ.
Report a problemFND-3430400901
08LabNot a test of 5-amino-1MQ aloneIn vitro (cell-free)Activity measurement
In lab tests, intra-day precision for 5-AMQ quantification in rat plasma and urine was within the 15 % acceptance range.
In plain wordsIn lab tests, intra-day precision for 5-AMQ quantification in rat plasma and urine was within the 15 % acceptance range.
Peptide
5-amino-1MQ
Outcome
Not applicableIntra-day precision
Study
ratn: not reported in source
rat plasma and urine assay
Outcome group
Pharmacokinetics · Pharmacology
Source sentences
Summary
The intra-day and inter-day precisions and accuracies for 5-AMQ at four concentration levels in rat plasma and urine samples were found to be within the 15 % FDA acceptance range.
In this study, 5-amino-1MQ was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking 5-amino-1MQ.
Report a problemFND-3430400902
09LabNot a test of 5-amino-1MQ aloneIn vitro (cell-free)Activity measurement
In lab tests, inter-day precision for 5-AMQ quantification in rat plasma and urine was within the 15 % acceptance range.
In plain wordsIn lab tests, inter-day precision for 5-AMQ quantification in rat plasma and urine was within the 15 % acceptance range.
Peptide
5-amino-1MQ
Outcome
Not applicableInter-day precision
Study
ratn: not reported in source
rat plasma and urine assay
Outcome group
Pharmacokinetics · Pharmacology
Source sentences
Summary
The intra-day and inter-day precisions and accuracies for 5-AMQ at four concentration levels in rat plasma and urine samples were found to be within the 15 % FDA acceptance range.
In this study, 5-amino-1MQ was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking 5-amino-1MQ.
Report a problemFND-3430400903
10LabNot a test of 5-amino-1MQ aloneIn vitro (cell-free)Activity measurement
In lab tests, intra-day accuracy for 5-AMQ quantification in rat plasma and urine was within the 15 % acceptance range.
In plain wordsIn lab tests, intra-day accuracy for 5-AMQ quantification in rat plasma and urine was within the 15 % acceptance range.
Peptide
5-amino-1MQ
Outcome
Not applicableIntra-day accuracy
Study
ratn: not reported in source
rat plasma and urine assay
Outcome group
Pharmacokinetics · Pharmacology
Source sentences
Summary
The intra-day and inter-day precisions and accuracies for 5-AMQ at four concentration levels in rat plasma and urine samples were found to be within the 15 % FDA acceptance range.
In this study, 5-amino-1MQ was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking 5-amino-1MQ.
Report a problemFND-3430400904
11LabNot a test of 5-amino-1MQ aloneIn vitro (cell-free)Activity measurement
In lab tests, inter-day accuracy for 5-AMQ quantification in rat plasma and urine was within the 15 % acceptance range.
In plain wordsIn lab tests, inter-day accuracy for 5-AMQ quantification in rat plasma and urine was within the 15 % acceptance range.
Peptide
5-amino-1MQ
Outcome
Not applicableInter-day accuracy
Study
ratn: not reported in source
rat plasma and urine assay
Outcome group
Pharmacokinetics · Pharmacology
Source sentences
Summary
The intra-day and inter-day precisions and accuracies for 5-AMQ at four concentration levels in rat plasma and urine samples were found to be within the 15 % FDA acceptance range.
In this study, 5-amino-1MQ was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking 5-amino-1MQ.
Report a problemFND-3430400905
12LabNot a test of 5-amino-1MQ aloneIn vitro (cell-free)Activity measurement
In lab tests, 5-AMQ quantification accuracy after dilution up to 20-fold was within the 15 % acceptable range.
In plain wordsIn lab tests, 5-AMQ quantification accuracy after dilution up to 20-fold was within the 15 % acceptable range.
Peptide
5-amino-1MQ
Outcome
Not applicableDilution accuracy
Study
ratn: not reported in source
rat plasma assay
Outcome group
Pharmacokinetics · Pharmacology
Source sentences
Summary
Similarly, the accuracy and precision of 5-AMQ quantification in samples diluted up to 20-fold using blank plasma were within the 15 % acceptable range.
In this study, 5-amino-1MQ was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking 5-amino-1MQ.
Report a problemFND-3430400906
13LabNot a test of 5-amino-1MQ aloneIn vitro (cell-free)Activity measurement
In lab tests, 5-AMQ quantification precision after dilution up to 20-fold was within the 15 % acceptable range.
In plain wordsIn lab tests, 5-AMQ quantification precision after dilution up to 20-fold was within the 15 % acceptable range.
Peptide
5-amino-1MQ
Outcome
Not applicableDilution precision
Study
ratn: not reported in source
rat plasma assay
Outcome group
Pharmacokinetics · Pharmacology
Source sentences
Summary
Similarly, the accuracy and precision of 5-AMQ quantification in samples diluted up to 20-fold using blank plasma were within the 15 % acceptable range.
In this study, 5-amino-1MQ was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking 5-amino-1MQ.
Report a problemFND-3430400907
14LabNot a test of 5-amino-1MQ aloneIn vitro (cell-free)Activity measurement
In lab tests, 5-AMQ extraction recovery from rat plasma ranged from 99.5 %-110.6 %.
In plain wordsIn lab tests, 5-AMQ extraction recovery from rat plasma ranged from 99.5 %-110.6 %.
Peptide
5-amino-1MQ
Outcome
Not applicableExtraction recovery
Study
ratn: not reported in source
rat plasma assay
Outcome group
Pharmacokinetics · Pharmacology
Measurements
extraction recovery
99.5 to 110.6%
rat plasma
Source sentences
Summary
Furthermore, the extraction recoveries and matrix effects at three concentration levels of rat plasma samples ranged from 99.5 %-110.6 % and -6.1 %-14.1 %, respectively.
In this study, 5-amino-1MQ was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking 5-amino-1MQ.
Report a problemFND-3430400908
15LabNot a test of 5-amino-1MQ aloneIn vitro (cell-free)Activity measurement
In lab tests, rat plasma matrix effects on 5-AMQ quantification ranged from -6.1 %-14.1 %.
In plain wordsIn lab tests, rat plasma matrix effects on 5-AMQ quantification ranged from -6.1 %-14.1 %.
Peptide
5-amino-1MQ
Outcome
Not applicableMatrix effects
Study
ratn: not reported in source
rat plasma assay
Outcome group
Pharmacokinetics · Pharmacology
Measurements
matrix effects
−6.1 to 14.1%
rat plasma
Source sentences
Summary
Furthermore, the extraction recoveries and matrix effects at three concentration levels of rat plasma samples ranged from 99.5 %-110.6 % and -6.1 %-14.1 %, respectively.
In this study, 5-amino-1MQ was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking 5-amino-1MQ.
Report a problemFND-3430400909
16LabNot a test of 5-amino-1MQ aloneIn vitro (cell-free)Activity measurement
In lab tests, 5-AMQ remained stable in rat plasma under standard storage and handling conditions, with less than 15 % variation.
In plain wordsIn lab tests, 5-AMQ remained stable in rat plasma under standard storage and handling conditions, with less than 15 % variation.
Peptide
5-amino-1MQ
Outcome
Not applicablePlasma sample stability
Study
ratn: not reported in source
rat plasma assay
Outcome group
Pharmacokinetics · Pharmacology
Measurements
variation
< 15%
rat plasma
Source sentences
Summary
5-AMQ was stable in rat plasma samples subjected to standard storage, preparation, and handling conditions, with less than 15 % variation noted at two concentration levels.
In this study, 5-amino-1MQ was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking 5-amino-1MQ.
Report a problemFND-3430400910

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
14 Oct 2026, 05:02 UTC
Study results updated
Version 1
14 Oct 2026, 01:46 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Journal Article

MeSH terms

Keywords

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