A quantitative UHPLC-MS/MS method for the growth hormone-releasing peptide-6 determination in complex biological matrices and transdermal formulations.

Esposito CL, Ac AG, Laszlo E, Duy SV, Michaud C, Sauvé S, Ong H, Marleau S, Banquy X, Brambilla D

Talanta26 May 2021Cited by 2 (Europe PMC)

PMID34215058DOI10.1016/j.talanta.2021.122555PepBankPUB-34215058

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study10

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone10

In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-6.

01LabNot a test of GHRP-6 aloneEx vivo tissueActivity measurement
In pig skin tests, GHRP‑6-loaded microneedle patches demonstrated the assay methods' validity and applicability.
In plain wordsIn lab tests with pig skin, GHRP‑6 microneedle patches demonstrated that the assay methods were applicable.
Peptide
GHRP-6
Outcome
Not applicableAssay applicability to transdermal delivery
Study
pign: not reported in source
Pig skin with GHRP-6-loaded microneedle patches
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
The validity and applicability of such methods were successfully demonstrated for transdermal GHRP-6 delivery using GHRP-6-loaded MN patches applied to pig skin.
1 context sentence(design, population, supporting numbers)
Summary · context
The ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) methods developed in this study aimed to quantify GHRP-6 in biological matrices (plasma, skin) and dissolving polymeric MNs.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-3421505810
02LabNot a test of GHRP-6 aloneIn vitro (cell-free)Activity measurement
In lab tests, the GHRP‑6 assay detection limit was 0.1 ng/mL in neat solution.
In plain wordsIn lab tests, the GHRP‑6 assay detected 0.1 ng/mL in neat solution.
Peptide
GHRP-6
Outcome
Not applicableDetection limit in neat solution
Study
n: not reported in source
UHPLC-MS/MS assay
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Detection limit
0.1 ng/mL
neat solution
Source sentences
Summary
UHPLC/MS-MS method detection limits of 0.1, 1.1, 0.9 and 1.5 ng/mL were achieved in neat solution, plasma, MN polymer solution, and skin matrices, respectively.
1 context sentence(design, population, supporting numbers)
Summary · context
The ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) methods developed in this study aimed to quantify GHRP-6 in biological matrices (plasma, skin) and dissolving polymeric MNs.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-3421505801
03LabNot a test of GHRP-6 aloneIn vitro (cell-free)Activity measurement
In lab tests, the GHRP‑6 assay detection limit was 1.1 ng/mL in plasma.
In plain wordsIn lab tests, the GHRP‑6 assay detected 1.1 ng/mL in plasma.
Peptide
GHRP-6
Outcome
Not applicableDetection limit in plasma
Study
n: not reported in source
UHPLC-MS/MS assay
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Detection limit
1.1 ng/mL
plasma
Source sentences
Summary
UHPLC/MS-MS method detection limits of 0.1, 1.1, 0.9 and 1.5 ng/mL were achieved in neat solution, plasma, MN polymer solution, and skin matrices, respectively.
1 context sentence(design, population, supporting numbers)
Summary · context
The ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) methods developed in this study aimed to quantify GHRP-6 in biological matrices (plasma, skin) and dissolving polymeric MNs.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-3421505802
04LabNot a test of GHRP-6 aloneIn vitro (cell-free)Activity measurement
In lab tests, the GHRP‑6 assay detection limit was 0.9 ng/mL in MN polymer solution.
In plain wordsIn lab tests, the GHRP‑6 assay detected 0.9 ng/mL in microneedle polymer solution.
Peptide
GHRP-6
Outcome
Not applicableDetection limit in polymer solution
Study
n: not reported in source
UHPLC-MS/MS assay
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
UHPLC/MS-MS method detection limits of 0.1, 1.1, 0.9 and 1.5 ng/mL were achieved in neat solution, plasma, MN polymer solution, and skin matrices, respectively.
1 context sentence(design, population, supporting numbers)
Summary · context
The ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) methods developed in this study aimed to quantify GHRP-6 in biological matrices (plasma, skin) and dissolving polymeric MNs.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-3421505803
05LabNot a test of GHRP-6 aloneIn vitro (cell-free)Activity measurement
In lab tests, the GHRP‑6 assay detection limit was 1.5 ng/mL in skin matrices.
In plain wordsIn lab tests, the GHRP‑6 assay detected 1.5 ng/mL in skin matrices.
Peptide
GHRP-6
Outcome
Not applicableDetection limit in skin matrices
Study
n: not reported in source
UHPLC-MS/MS assay
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
UHPLC/MS-MS method detection limits of 0.1, 1.1, 0.9 and 1.5 ng/mL were achieved in neat solution, plasma, MN polymer solution, and skin matrices, respectively.
1 context sentence(design, population, supporting numbers)
Summary · context
The ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) methods developed in this study aimed to quantify GHRP-6 in biological matrices (plasma, skin) and dissolving polymeric MNs.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-3421505804
06LabNot a test of GHRP-6 aloneIn vitro (cell-free)Activity measurement
In lab tests, matched calibration curves for GHRP‑6 had R-squared greater than 0.990.
In plain wordsIn lab tests, GHRP‑6 calibration curves had R-squared greater than 0.990.
Peptide
GHRP-6
Outcome
Not applicableCalibration linearity
Study
n: not reported in source
UHPLC-MS/MS assay
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
Method validation also involved assessment of precision, accuracy, limits of quantification, linearity of matched calibration curves (R² > 0.990), extraction recovery, matrix effect, stability studies, selectivity, and carry-over effect.
1 context sentence(design, population, supporting numbers)
Summary · context
The ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) methods developed in this study aimed to quantify GHRP-6 in biological matrices (plasma, skin) and dissolving polymeric MNs.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-3421505805
07LabNot a test of GHRP-6 aloneIn vitro (cell-free)Activity measurement
In lab tests, GHRP‑6 quality-control recovery ranged from 85-109%.
In plain wordsIn lab tests, GHRP‑6 quality-control recovery ranged from 85-109%.
Peptide
GHRP-6
Outcome
Not applicableRecovery
Study
n: not reported in source
UHPLC-MS/MS assay
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
Additionally, quality control samples were analyzed at three concentration levels to determine recovery (85-109%) and accuracy/bias (3.2-14.7%).
1 context sentence(design, population, supporting numbers)
Summary · context
The ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) methods developed in this study aimed to quantify GHRP-6 in biological matrices (plasma, skin) and dissolving polymeric MNs.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-3421505806
08LabNot a test of GHRP-6 aloneIn vitro (cell-free)Activity measurement
In lab tests, GHRP‑6 quality-control accuracy/bias ranged from 3.2-14.7%.
In plain wordsIn lab tests, GHRP‑6 assay accuracy/bias ranged from 3.2-14.7%.
Peptide
GHRP-6
Outcome
Not applicableAccuracy/bias
Study
n: not reported in source
UHPLC-MS/MS assay
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
Additionally, quality control samples were analyzed at three concentration levels to determine recovery (85-109%) and accuracy/bias (3.2-14.7%).
1 context sentence(design, population, supporting numbers)
Summary · context
The ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) methods developed in this study aimed to quantify GHRP-6 in biological matrices (plasma, skin) and dissolving polymeric MNs.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-3421505807
09LabNot a test of GHRP-6 aloneIn vitro (cell-free)Activity measurement
In lab tests, GHRP‑6 assay intra-day precision had RSDs of 3.0-13.9%, within acceptance limits.
In plain wordsIn lab tests, GHRP‑6 assay within-day precision met acceptance limits.
Peptide
GHRP-6
Outcome
Not applicableIntra-day precision
Study
n: not reported in source
UHPLC-MS/MS assay
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
Intra- and inter-day precision were within the range of acceptance (RSDs of 3.0-13.9% and 0.4-14.5%, respectively).
1 context sentence(design, population, supporting numbers)
Summary · context
The ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) methods developed in this study aimed to quantify GHRP-6 in biological matrices (plasma, skin) and dissolving polymeric MNs.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-3421505808
10LabNot a test of GHRP-6 aloneIn vitro (cell-free)Activity measurement
In lab tests, GHRP‑6 assay inter-day precision had RSDs of 0.4-14.5%, within acceptance limits.
In plain wordsIn lab tests, GHRP‑6 assay between-day precision met acceptance limits.
Peptide
GHRP-6
Outcome
Not applicableInter-day precision
Study
n: not reported in source
UHPLC-MS/MS assay
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
Intra- and inter-day precision were within the range of acceptance (RSDs of 3.0-13.9% and 0.4-14.5%, respectively).
1 context sentence(design, population, supporting numbers)
Summary · context
The ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) methods developed in this study aimed to quantify GHRP-6 in biological matrices (plasma, skin) and dissolving polymeric MNs.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-3421505809

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
9 Oct 2026, 03:13 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Journal Article

MeSH terms

Keywords

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