In C2C12 cells, Des(1‑3) IGF-I did not affect porcine MYH4 promoter activity compared with vehicle controls.
In plain wordsIn cells, Des(1‑3) IGF-I did not change porcine MYH4 promoter activity compared with vehicle controls.
- Peptide
- IGF-1 DES (des(1-3)IGF-I)
- Outcome
- No significant changePorcine MYH4 promoter activity
- Study
mousen: not reported in source
C2C12 myotubes- Comparator
- Vehicle controls
- Outcome group
- Receptor activity and mechanism · Musculoskeletal
- Studied dose
- Dose not stated · four daysAs given in this study (mouse).
Source sentences
Porcine MYH4-promoter activity also tended to be increased by T3 treatment, but there were no effects of Des(1-3) IGF-I or Dex treatment, whereas average myotube diameter was increased by Des(1-3) IGF-I, but not T3 or Dex.
2 context sentences(design, population, supporting numbers)
C2C12 myoblasts were co-transfected with porcine MYH4-promoter-driven ZsGreen and CMV-driven DsRed expression plasmids.
At the onset of differentiation, treatments (dibutyryl cyclic-AMP (dbcAMP), Des(1-3) Insulin-Like Growth Factor-1 (IGF-I), triiodo-l-thyronine (T3) and dexamethasone (Dex)) or appropriate vehicle controls were added and cells maintained for up to four days.