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Study · In vitro (cells) · mouse · 2020

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Compound C Protects Against Cisplatin-Induced Nephrotoxicity Through Pleiotropic Effects.

Li F, Sun A, Cheng G, Liu D, Xiao J, Zhao Z, Dong Z

Frontiers in physiology23 Dec 2020Cited by 14 (Europe PMC)

PMID33424637 (opens in a new tab)PMCIDPMC7785967 (opens in a new tab)DOI10.3389/fphys.2020.614244 (opens in a new tab)PepBankPUB-33424637
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Summary

AICAR (Acadesine/AICA riboside) as an activator of AMPK, can protect renal tubular cells from cisplatin induced apoptosis. But in our experiment, the dorsomorphin (compound C, an inhibitor of AMPK) also significantly reduced cisplatin induced renal tubular cells apoptosis. Accordingly, we tested whether compound C can protect cisplatin-induced nephrotoxicity and the specific mechanism. Here, we treated Boston University mouse proximal tubular cells (BUMPT-306) with cisplatin and/or different dosages of AICAR (Acadesine/AICA riboside) or compound C to confirm the effect of AICAR and compound C in vitro. The AMPK-siRNA treated cells to evaluate whether the protective effect of compound C was through inhibiting AMPK. Male C57BL/6 mice were used to verify the effect of compound C in vivo. Both compound C and AICAR can reduce renal tubular cells apoptosis in dose-dependent manners, and compound C decreased serum creatinine and renal tubular injury induced by cisplatin. Mechanistically, compound C inhibited P53, CHOP and p-IREα during cisplatin treatment. Our results demonstrated that compound C inhibited AMPK, but the renal protective effects of compound C were not through AMPK. Instead, compound C protected cisplatin nephrotoxicity by inhibiting P53 and endoplasmic reticulum (ER) stress. Therefore, compound C may protect against cisplatin-induced nephrotoxicity through pleiotropic effects.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study1

1 result from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    In cisplatin-treated renal tubular cells, AICAR reduced apoptosis in a dose-dependent manner.

    In plain wordsIn lab tests, AICAR reduced cell death in kidney cells exposed to cisplatin, with a dose-dependent effect.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseRenal tubular cell apoptosis
    Study

    mousen: not reported in source

    BUMPT-306 renal tubular cells
    Outcome group
    Kidney function · Kidney and urinary
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Both compound C and AICAR can reduce renal tubular cells apoptosis in dose-dependent manners
    1 context sentence
    Summary · context
    Here, we treated Boston University mouse proximal tubular cells (BUMPT-306) with cisplatin and/or different dosages of AICAR (Acadesine/AICA riboside) or compound C to confirm the effect of AICAR and compound C in vitro.

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Version history

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  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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