Mass spectrometric and kinetics characterization of modified species of Growth Hormone Releasing Hexapeptide generated under thermal stress in different pH and buffers.

Santana H, Espinosa LA, Sánchez A, Bolaño Alvarez A, Besada V, González LJ

Journal of pharmaceutical and biomedical analysis21 Nov 2020Cited by 4 (Europe PMC)

PMID33272786DOI10.1016/j.jpba.2020.113776PepBankPUB-33272786

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone6

In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-6.

01LabNot a test of GHRP-6 aloneIn vitro (cell-free)Other
In lab tests, GHRP‑6 C-terminal deamidation occurred preferentially at pH 3.0 and 8.0.
In plain wordsIn lab tests, GHRP‑6 deamidation occurred preferentially at pH 3.0 and 8.0.
Peptide
GHRP-6
Outcome
Not applicableC-terminal deamidation
Study
n: not reported in source
GHRP-6 under thermal stress in buffers
Outcome group
Formulation, delivery and stability · Pharmacology
Source sentences
Summary
C-terminal deamidation of GHRP-6 was generated preferentially at pH 3.0 and 8.0.
1 context sentence(design, population, supporting numbers)
Summary · context
Degradation products (DPs) of GHRP-6, generated by heating in the dark at 60 °C with pH ranging from 3.0 to 8.0 and in presence of common buffers, were isolated by RP-HPLC and characterized by ESI-MS/MS.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-3327278601
02LabNot a test of GHRP-6 aloneIn vitro (cell-free)Other
In lab tests, phosphate buffers at pH 6.0 to 7.0 favored GHRP‑6 hydrolysis.
In plain wordsIn lab tests, phosphate buffers at pH 6.0 to 7.0 favored GHRP‑6 breakdown through hydrolysis.
Peptide
GHRP-6
Outcome
Not applicableHydrolysis
Study
n: not reported in source
GHRP-6 under thermal stress in buffers
Outcome group
Formulation, delivery and stability · Pharmacology
Source sentences
Summary
Hydrolysis and head-to-tail cyclization were favored at pH ranging from 6.0 to 7.0 in phosphate containing buffers.
1 context sentence(design, population, supporting numbers)
Summary · context
Degradation products (DPs) of GHRP-6, generated by heating in the dark at 60 °C with pH ranging from 3.0 to 8.0 and in presence of common buffers, were isolated by RP-HPLC and characterized by ESI-MS/MS.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-3327278602
03LabNot a test of GHRP-6 aloneIn vitro (cell-free)Other
In lab tests, phosphate buffers at pH 6.0 to 7.0 favored GHRP‑6 head-to-tail cyclization.
In plain wordsIn lab tests, phosphate buffers at pH 6.0 to 7.0 favored formation of cyclic GHRP‑6.
Peptide
GHRP-6
Outcome
Not applicableHead-to-tail cyclization
Study
n: not reported in source
GHRP-6 under thermal stress in buffers
Outcome group
Formulation, delivery and stability · Pharmacology
Source sentences
Summary
Hydrolysis and head-to-tail cyclization were favored at pH ranging from 6.0 to 7.0 in phosphate containing buffers.
1 context sentence(design, population, supporting numbers)
Summary · context
Degradation products (DPs) of GHRP-6, generated by heating in the dark at 60 °C with pH ranging from 3.0 to 8.0 and in presence of common buffers, were isolated by RP-HPLC and characterized by ESI-MS/MS.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-3327278603
04LabNot a test of GHRP-6 aloneIn vitro (cell-free)Other
In lab tests, a GHRP‑6 degradation product had a +12 Da mass shift, presumably from a reaction with formaldehyde.
In plain wordsIn lab tests, a GHRP‑6 degradation product gained +12 Da, presumably through a reaction with formaldehyde.
Peptide
GHRP-6
Outcome
Not applicableDegradation product mass shift
Study
n: not reported in source
GHRP-6 under thermal stress in buffers
Outcome group
Formulation, delivery and stability · Pharmacology
Source sentences
Summary
A DP with +12 Da molecular mass was presumably originated by the reaction with formaldehyde derived from some of the additives and/or elastomeric closures.
1 context sentence(design, population, supporting numbers)
Summary · context
Degradation products (DPs) of GHRP-6, generated by heating in the dark at 60 °C with pH ranging from 3.0 to 8.0 and in presence of common buffers, were isolated by RP-HPLC and characterized by ESI-MS/MS.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-3327278604
05LabNot a test of GHRP-6 aloneIn vitro (cell-free)Other
In lab tests, pH 3.0-6.0 favored GHRP‑6 degradation products from tri- and di-carboxylic buffer acylation.
In plain wordsIn lab tests, pH 3.0-6.0 favored GHRP‑6 chemical modification by carboxylic buffers.
Peptide
GHRP-6
Outcome
Not applicableBuffer-derived acylation
Study
n: not reported in source
GHRP-6 under thermal stress in buffers
Outcome group
Formulation, delivery and stability · Pharmacology
Source sentences
Summary
Certain DPs derived from the acylation reaction of the tri- and di-carboxylic buffering species were favored at pH 3.0-6.0 and indicate that buffer components, including those "Generally Recognized as Safe", may potentially introduce chemical modifications and product heterogeneity.
1 context sentence(design, population, supporting numbers)
Summary · context
Degradation products (DPs) of GHRP-6, generated by heating in the dark at 60 °C with pH ranging from 3.0 to 8.0 and in presence of common buffers, were isolated by RP-HPLC and characterized by ESI-MS/MS.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-3327278605
06LabNot a test of GHRP-6 aloneIn vitro (cell-free)Other
In lab tests, low-abundance GHRP‑6 species contained tryptophan oxidized to 5-hydroxy, kynurenine, and N-formylkynurenine.
In plain wordsIn lab tests, low-abundance GHRP‑6 species contained several oxidized forms of tryptophan.
Peptide
GHRP-6
Outcome
Not applicableTryptophan oxidation products
Study
n: not reported in source
GHRP-6 under thermal stress in buffers
Outcome group
Formulation, delivery and stability · Pharmacology
Source sentences
Summary
Nano LC-MS/MS analysis revealed GHRP-6 was also detected as a low-abundance species with Trp oxidized to 5-hydroxy, kynurenine, and N-formylkynurenine.
1 context sentence(design, population, supporting numbers)
Summary · context
Degradation products (DPs) of GHRP-6, generated by heating in the dark at 60 °C with pH ranging from 3.0 to 8.0 and in presence of common buffers, were isolated by RP-HPLC and characterized by ESI-MS/MS.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-3327278606

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
9 Oct 2026, 03:13 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Journal Article

MeSH terms

Keywords

PepBank is a research reference. Talk to a doctor before you use any peptide.