PTH(1-34) treatment and/or mechanical loading have different osteogenic effects on the trabecular and cortical bone in the ovariectomized C57BL/6 mouse.

Roberts BC, Arredondo Carrera HM, Zanjani-Pour S, Boudiffa M, Wang N, Gartland A, Dall'Ara E

Scientific reports1 Jun 2020Cited by 29 (Europe PMC)

PMID32483372PMCIDPMC7264307DOI10.1038/s41598-020-65921-1PepBankPUB-32483372

Summary

In preclinical mouse models, a synergistic anabolic response to PTH(1-34) and tibia loading was shown. Whether combined treatment improves bone properties with oestrogen deficiency, a cardinal feature of osteoporosis, remains unknown. This study quantified the individual and combined longitudinal effects of PTH(1-34) and loading on the bone morphometric and densitometric properties in ovariectomised mice. C57BL/6 mice were ovariectomised at 14-weeks-old and treated either with injections of PTH(1-34); compressive loading of the right tibia; both interventions concurrently; or both interventions on alternating weeks. Right tibiae were microCT-scanned from 14 until 24-weeks-old. Trabecular metaphyseal and cortical midshaft morphometric properties, and bone mineral content (BMC) in 40 different regions of the tibia were measured. Mice treated only with loading showed the highest trabecular bone volume fraction at week 22. Cortical thickness was higher with co-treatment than in the mice treated with PTH alone. In the mid-diaphysis, increases in BMC were significantly higher with loading than PTH. In ovariectomised mice, the osteogenic benefits of co-treatment on the trabecular bone were lower than loading alone. However, combined interventions had increased, albeit regionally-dependent, benefits to cortical bone. Increased benefits were largest in the mid-diaphysis and postero-laterally, regions subjected to higher strains under compressive loads.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study5

3 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In ovariectomised mice, PTH produced smaller mid-diaphyseal bone mineral content increases than mechanical loading.
In plain wordsIn mice with ovaries removed, PTH increased middle-shaft bone mineral content less than mechanical loading.
Peptide
Teriparatide (PTH 1-34)
Outcome
DecreaseMid-diaphyseal bone mineral content increase
Study
mousen: not reported in source
Ovariectomised C57BL/6 mice
Comparator
Mechanical loading
Outcome group
Bone and joint · Musculoskeletal
Studied dose
Dose not stated · injectionAs given in this study (mouse).
Source sentences
Summary
In the mid-diaphysis, increases in BMC were significantly higher with loading than PTH.
2 context sentences(design, population, supporting numbers)
Summary · context
This study quantified the individual and combined longitudinal effects of PTH(1-34) and loading on the bone morphometric and densitometric properties in ovariectomised mice.
Summary · context
C57BL/6 mice were ovariectomised at 14-weeks-old and treated either with injections of PTH(1-34); compressive loading of the right tibia; both interventions concurrently; or both interventions on alternating weeks.
Show in the study text Report a problemFND-3248337202
02AnimalAnimal study (in vivo)Efficacy outcome
In ovariectomised mice, adding PTH(1-34) to loading produced lower trabecular osteogenic benefits than loading alone.
In plain wordsIn mice with ovaries removed, PTH with loading produced less trabecular bone benefit than loading alone.
Peptide
Teriparatide (PTH 1-34)
Outcome
DecreaseTrabecular osteogenic benefit with loading
Study
mousen: not reported in source
Ovariectomised C57BL/6 mice
Comparator
Loading alone
Outcome group
Bone and joint · Musculoskeletal
Studied dose
Dose not stated · injectionAs given in this study (mouse).
Source sentences
Summary
In ovariectomised mice, the osteogenic benefits of co-treatment on the trabecular bone were lower than loading alone.
2 context sentences(design, population, supporting numbers)
Summary · context
This study quantified the individual and combined longitudinal effects of PTH(1-34) and loading on the bone morphometric and densitometric properties in ovariectomised mice.
Summary · context
C57BL/6 mice were ovariectomised at 14-weeks-old and treated either with injections of PTH(1-34); compressive loading of the right tibia; both interventions concurrently; or both interventions on alternating weeks.
Show in the study text Report a problemFND-3248337203
03AnimalAnimal study (in vivo)Efficacy outcome
In ovariectomised mice, PTH-only treatment had lower trabecular bone volume fraction than loading alone at week 22.
In plain wordsIn mice with ovaries removed, PTH alone produced lower trabecular bone volume fraction than loading alone at week 22.
Peptide
Teriparatide (PTH 1-34)
Outcome
DecreaseTrabecular bone volume fraction at week 22
Study
mousen: not reported in source
Ovariectomised C57BL/6 mice
Comparator
Mechanical loading alone
Outcome group
Bone and joint · Musculoskeletal
Studied dose
Dose not stated · injectionAs given in this study (mouse).
Source sentences
Summary
Mice treated only with loading showed the highest trabecular bone volume fraction at week 22.
2 context sentences(design, population, supporting numbers)
Summary · context
C57BL/6 mice were ovariectomised at 14-weeks-old and treated either with injections of PTH(1-34); compressive loading of the right tibia; both interventions concurrently; or both interventions on alternating weeks.
Summary · context
This study quantified the individual and combined longitudinal effects of PTH(1-34) and loading on the bone morphometric and densitometric properties in ovariectomised mice.
Show in the study text Report a problemFND-3248337205

Studies that did not test the peptide alone2

In this study, Teriparatide (PTH 1-34) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Teriparatide (PTH 1-34).

04AnimalNot a test of Teriparatide (PTH 1-34) aloneAnimal study (in vivo)Efficacy outcome
In ovariectomised mice, PTH(1-34) with mechanical loading produced greater cortical thickness than PTH alone.
In plain wordsIn mice with ovaries removed, PTH with mechanical loading produced thicker cortical bone than PTH alone.
Peptide
Teriparatide (PTH 1-34)
Outcome
IncreaseCortical thickness
Study
mousen: not reported in source
Ovariectomised C57BL/6 mice
Comparator
PTH(1-34) alone
Outcome group
Bone and joint · Musculoskeletal
Source sentences
Summary
Cortical thickness was higher with co-treatment than in the mice treated with PTH alone.
2 context sentences(design, population, supporting numbers)
Summary · context
This study quantified the individual and combined longitudinal effects of PTH(1-34) and loading on the bone morphometric and densitometric properties in ovariectomised mice.
Summary · context
C57BL/6 mice were ovariectomised at 14-weeks-old and treated either with injections of PTH(1-34); compressive loading of the right tibia; both interventions concurrently; or both interventions on alternating weeks.
In this study, Teriparatide (PTH 1-34) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Teriparatide (PTH 1-34).
Show in the study text Report a problemFND-3248337201
05AnimalNot a test of Teriparatide (PTH 1-34) aloneAnimal study (in vivo)Efficacy outcome
In ovariectomised mice, combined PTH(1-34) and loading had region-dependent cortical bone benefits, greatest in mid-diaphyseal and posterolateral regions.
In plain wordsIn mice with ovaries removed, combined PTH and loading benefited cortical bone most in middle-shaft and posterolateral regions.
Peptide
Teriparatide (PTH 1-34)
Outcome
IncreaseRegional cortical bone benefit
Study
mousen: not reported in source
Ovariectomised C57BL/6 mice
Outcome group
Bone and joint · Musculoskeletal
Source sentences
Summary
However, combined interventions had increased, albeit regionally-dependent, benefits to cortical bone. Increased benefits were largest in the mid-diaphysis and postero-laterally, regions subjected to higher strains under compressive loads.
2 context sentences(design, population, supporting numbers)
Summary · context
This study quantified the individual and combined longitudinal effects of PTH(1-34) and loading on the bone morphometric and densitometric properties in ovariectomised mice.
Summary · context
C57BL/6 mice were ovariectomised at 14-weeks-old and treated either with injections of PTH(1-34); compressive loading of the right tibia; both interventions concurrently; or both interventions on alternating weeks.
In this study, Teriparatide (PTH 1-34) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Teriparatide (PTH 1-34).
Show in the study text Report a problemFND-3248337204

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
22 Oct 2026, 02:26 UTC
Study results updated
Version 1
21 Oct 2026, 18:59 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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MeSH terms

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