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Study · Ex vivo tissue · rat · 2020

2 results

Acute bout of exercise downregulates thioredoxin-interacting protein expression in rat contracting skeletal muscles.

Ra SG, Kawamoto E, Koshinaka K, Iwabe M, Tomiga Y, Iizawa H, Honda H, Higaki Y, Kawanaka K

Physiological reports1 Jun 2020Cited by 7 (Europe PMC)

PMID32476292 (opens in a new tab)PMCIDPMC7261653 (opens in a new tab)DOI10.14814/phy2.14388 (opens in a new tab)PepBankPUB-32476292
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Summary

We previously reported that in rat skeletal muscle, disuse (i.e., decreased muscle contractile activity) rapidly increases thioredoxin-interacting protein (TXNIP), which is implicated in the reduced glucose uptake. Accordingly, we sought herein to (a) determine the effect of exercise (i.e., increased muscle contractile activity) on muscle TXNIP protein expression, and (b) elucidate the mechanisms underlying the changes of TXNIP protein expression in response to exercise. Rat epitrochlearis and soleus muscles were dissected out after an acute bout of 3-hr swimming (without weight loading) or 3-hr treadmill running (15% grade at 9m/min). In a separate protocol, the isolated epitrochlearis and soleus muscles were incubated for 3 hr with AMP-dependent protein kinase activator AICAR. Immediately after the cessation of the 3-hr swimming, the TXNIP protein was decreased in epitrochlearis but not in soleus muscle. Conversely, 3-hr treadmill running decreased the TXNIP protein in soleus but not in epitrochlearis muscle. TXNIP protein was decreased concomitantly with reduced postexercise muscle glycogen, showing that a decrease in TXNIP protein expression occurs in muscles that are recruited during exercise. In addition, 3-hr incubation with AICAR decreased TXNIP protein in both isolated epitrochlearis and soleus muscles. Our results suggest that (a) an acute bout of exercise downregulates TXNIP protein expression in rat contracting skeletal muscles, and (b) the reduction in TXNIP protein expression in contracting muscles is probably mediated by AMPK activation, at least in part.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study2

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueMechanism

    In isolated rat epitrochlearis muscle, AICAR incubation decreased TXNIP protein.

    In plain wordsIn lab tests with rat epitrochlearis muscle, AICAR decreased TXNIP protein.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseEpitrochlearis TXNIP protein
    Study

    ratn: not reported in source

    isolated epitrochlearis muscle
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    Dose not stated · 3 hrAs given in this study (rat).

    Source sentences

    Summary
    In addition, 3-hr incubation with AICAR decreased TXNIP protein in both isolated epitrochlearis and soleus muscles.
    1 context sentence
    Summary · context
    In a separate protocol, the isolated epitrochlearis and soleus muscles were incubated for 3 hr with AMP-dependent protein kinase activator AICAR.
  2. 02LabEx vivo tissueMechanism

    In isolated rat soleus muscle, AICAR incubation decreased TXNIP protein.

    In plain wordsIn lab tests with rat soleus muscle, AICAR decreased TXNIP protein.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseSoleus TXNIP protein
    Study

    ratn: not reported in source

    isolated soleus muscle
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    Dose not stated · 3 hrAs given in this study (rat).

    Source sentences

    Summary
    In addition, 3-hr incubation with AICAR decreased TXNIP protein in both isolated epitrochlearis and soleus muscles.
    1 context sentence
    Summary · context
    In a separate protocol, the isolated epitrochlearis and soleus muscles were incubated for 3 hr with AMP-dependent protein kinase activator AICAR.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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