Skip to content

Study · Ex vivo tissue · mouse · 2020

4 results

Melanocortin regulation of histaminergic neurons via perifornical lateral hypothalamic melanocortin 4 receptors.

Michael NJ, Caron A, Lee CE, Castorena CM, Lee S, Zigman JM, Williams KW, Elmquist JK

Molecular metabolism4 Feb 2020Cited by 10 (Europe PMC)

PMID32244183 (opens in a new tab)PMCIDPMC7082550 (opens in a new tab)DOI10.1016/j.molmet.2020.01.020 (opens in a new tab)PepBankPUB-32244183
Read at sourcePubMedAsk about this study
Jump to 4 results

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: CC BY-NC-ND. Results still quote short attributed sentences.

Results from this study4

3 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueActivity measurement

    In mouse brain tissue, melanotan II excited subsets of histidine decarboxylase-expressing neurons.

    In plain wordsIn lab tests on mouse brain tissue, melanotan II excited some histamine-producing nerve cells.

    Peptide
    Melanotan II
    Outcome
    IncreaseExcitation of HDC-expressing neurons by melanotan II
    Study

    mousen: not reported in source

    HDC-expressing neurons, whole-cell patch-clamp
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    We found that subsets of HDC-expressing neurons were excited by melanotan II (MTII), a non-selective melanocortin receptor agonist.
    1 context sentence
    Summary · context
    We utilized Hdc-Cre mice in combination with whole-cell patch-clamp electrophysiology and in vivo chemogenetic techniques to determine whether HDC neurons receive metabolically relevant information via the melanocortin system.
  2. 02LabEx vivo tissueMechanism

    In mouse brain tissue, selective agonists and synaptic blockers indicated that the melanotan II effect on HDC neurons was mediated by MC4Rs through a glutamate-dependent presynaptic mechanism.

    In plain wordsIn lab tests on mouse brain tissue, the melanotan II effect on these nerve cells worked through MC4 receptors and a glutamate-dependent mechanism.

    Peptide
    Melanotan II
    Outcome
    Not applicableReceptor and synaptic mechanism of the melanotan II effect on HDC neurons
    Study

    mousen: not reported in source

    HDC-expressing neurons, whole-cell patch-clamp
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Use of melanocortin receptor selective agonists (THIQ, [D-Trp8]-γ-MSH) and inhibitors of synaptic transmission (TTX, CNQX, AP5) indicated that the effect was mediated specifically by MC4Rs and involved a glutamatergic dependent presynaptic mechanism.
    1 context sentence
    Summary · context
    We utilized Hdc-Cre mice in combination with whole-cell patch-clamp electrophysiology and in vivo chemogenetic techniques to determine whether HDC neurons receive metabolically relevant information via the melanocortin system.
  3. 03LabEx vivo tissueActivity measurement

    In mouse brain tissue, melanotan II enhanced evoked excitatory post-synaptic currents from stimulation of the perifornical lateral hypothalamic area.

    In plain wordsIn lab tests on mouse brain tissue, melanotan II strengthened excitatory signals from a nearby brain area to the histamine neurons.

    Peptide
    Melanotan II
    Outcome
    IncreaseEvoked excitatory post-synaptic currents after perifornical lateral hypothalamic stimulation
    Study

    mousen: not reported in source

    Brain slices, electrical stimulation of the perifornical lateral hypothalamic area
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    MTII enhanced evoked excitatory post-synaptic currents (EPSCs) originating from electrical stimulation of the perifornical lateral hypothalamic area (PeFLH), supportive of melanocortin effects on the glutamatergic PeFLH projection to the TMN.
    1 context sentence
    Summary · context
    We utilized Hdc-Cre mice in combination with whole-cell patch-clamp electrophysiology and in vivo chemogenetic techniques to determine whether HDC neurons receive metabolically relevant information via the melanocortin system.

Studies that did not test the peptide alone1

In this study, Melanotan II was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Melanotan II.

  1. 04AnimalNot a test of Melanotan II aloneAnimal study (in vivo)Efficacy outcome

    In mice, chemogenetic inhibition of HDC neurons enhanced the food-intake suppressing effect of intracerebroventricular melanotan II.

    In plain wordsIn mice, silencing histamine nerve cells made the appetite-reducing effect of melanotan II stronger.

    Peptide
    Melanotan II
    Outcome
    IncreaseAnorexigenic effect of intracerebroventricular melanotan II with chemogenetic inhibition of HDC neurons
    Study

    mousen: not reported in source

    Hdc-Cre mice, chemogenetic inhibition
    Outcome group
    Appetite and food intake · Metabolic and weight

    Source sentences

    Summary
    Finally, in vivo chemogenetic inhibition of HDC neurons strikingly enhanced the anorexigenic effects of intracerebroventricular administration of MTII, suggesting that MC4R activation of histaminergic neurons may restrain the anorexigenic effects of melanocortin system activation.
    1 context sentence
    Summary · context
    We utilized Hdc-Cre mice in combination with whole-cell patch-clamp electrophysiology and in vivo chemogenetic techniques to determine whether HDC neurons receive metabolically relevant information via the melanocortin system.

    In this study, Melanotan II was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan II.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.