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Study · Animal study (in vivo) · rat · 2019

3 results

Regeneration of Denervated Skeletal Muscles - Brunelli's CNS-PNS Paradigm.

von Wild T, Brunelli GA, von Wild KRH, Löhnhardt M, Catoi C, Catoi AF, Vester JC, Strilciuc S, Trillenberg P

Journal of medicine and life1 Oct 2019

PMID32025252 (opens in a new tab)PMCIDPMC6993288 (opens in a new tab)DOI10.25122/jml-2019-0063 (opens in a new tab)PepBankPUB-32025252
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Summary

The restoration of voluntary muscle activity in posttraumatic paraplegia in both animal experiments and other clinical applications requires reproducibility of a technically-demanding microsurgical procedure, limited by physicians' understanding of Brunelli's spinal cord grafting paradigm. The insufficient clinical investigation of the long-term benefits of the CNS-PNS graft application warrants additional inquiry. The objective of this study is to explore the potential benefits of the first replicated, graft-induced neuroregeneration of denervated skeletal muscle regarding long-term clinical outcomes and to investigate the effect of Cerebrolysin on neuromodulation. A randomized study evaluating 30 rats, approved by the National Animal Ethics Advisory Committee was performed. The medication was administered postoperatively. For 14 days, 12 rats received Cerebrolysin (serum), 11 received NaCl 0.9% (shams), and 7 were controls. For microsurgery, the lateral corticospinal tract T10 was grafted to the denervated internal obliquus abdominal muscle. On day 90, intraoperative proof of reinnervation was observed. On day 100, 15 rats were euthanized for fixation, organ removal, and extensive histology-morphology examination, and the Wei-Lachin statistical procedure was employed. After an open revision of 16 rats, 8 were CMAP positive. After intravenous Vecuronium application, two (Cerebrolysin, NaCl) out of two rats showed an incomplete compound muscle action potential (CMAP) loss due to glutamatergic and cholinergic co-transmission, while two others showed a complete loss of amplitude. Cerebrolysin medication initiated larger restored muscle fiber diameters and less scarring. FB+ neurons were not observed in the brain but were observed in the Rexed laminae. Brunelli's concept was successfully replicated, demonstrating the first graft induced existence of cholinergic and glutamatergic neurotransmission in denervated grafted muscles. Statistics of the histometric count of muscle fibers revealed larger fiber diameters after Cerebrolysin. Brunelli's CNS-PNS experimental concept is suitable to analyze graft-neuroplasticity focused on the voluntary restoration of denervated skeletal muscles in spinal cord injury. Neuroprotection by Cerebrolysin is demonstrated.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study3

3 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Mechanism

    In grafted rats, incomplete CMAP loss after vecuronium occurred in both a Cerebrolysin rat and a NaCl rat.

    In plain wordsIn rats, incomplete muscle signal loss after vecuronium occurred with both Cerebrolysin and NaCl.

    Peptide
    Cerebrolysin
    Outcome
    Not applicableIncomplete CMAP loss after vecuronium
    Study

    ratn = 2

    CNS-PNS graft to denervated internal obliquus abdominal muscle
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    Dose not stated · 14 daysAs given in this study (rat).

    Source sentences

    Summary
    After intravenous Vecuronium application, two (Cerebrolysin, NaCl) out of two rats showed an incomplete compound muscle action potential (CMAP) loss due to glutamatergic and cholinergic co-transmission, while two others showed a complete loss of amplitude.
    2 context sentences
    Summary · context
    For 14 days, 12 rats received Cerebrolysin (serum), 11 received NaCl 0.9% (shams), and 7 were controls.
    Summary · context
    For microsurgery, the lateral corticospinal tract T10 was grafted to the denervated internal obliquus abdominal muscle.
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In rats with denervated grafted muscles, Cerebrolysin increased restored muscle fiber diameters.

    In plain wordsIn rats with denervated grafted muscles, Cerebrolysin increased restored muscle fiber diameters.

    Peptide
    Cerebrolysin
    Outcome
    IncreaseRestored muscle fiber diameter
    Study

    ratn: not reported in source

    CNS-PNS graft to denervated internal obliquus abdominal muscle
    Outcome group
    Muscle injury and repair · Musculoskeletal
    Studied dose
    Dose not stated · 14 daysAs given in this study (rat).

    Source sentences

    Summary
    Cerebrolysin medication initiated larger restored muscle fiber diameters and less scarring.
    3 context sentences
    Summary · context
    Statistics of the histometric count of muscle fibers revealed larger fiber diameters after Cerebrolysin.
    Summary · context
    For 14 days, 12 rats received Cerebrolysin (serum), 11 received NaCl 0.9% (shams), and 7 were controls.
    Summary · context
    For microsurgery, the lateral corticospinal tract T10 was grafted to the denervated internal obliquus abdominal muscle.
  3. 03AnimalAnimal study (in vivo)Efficacy outcome

    In rats with denervated grafted muscles, Cerebrolysin reduced scarring.

    In plain wordsIn rats with denervated grafted muscles, Cerebrolysin reduced scarring.

    Peptide
    Cerebrolysin
    Outcome
    DecreaseMuscle scarring
    Study

    ratn: not reported in source

    CNS-PNS graft to denervated internal obliquus abdominal muscle
    Outcome group
    Muscle injury and repair · Musculoskeletal
    Studied dose
    Dose not stated · 14 daysAs given in this study (rat).

    Source sentences

    Summary
    Cerebrolysin medication initiated larger restored muscle fiber diameters and less scarring.
    2 context sentences
    Summary · context
    For 14 days, 12 rats received Cerebrolysin (serum), 11 received NaCl 0.9% (shams), and 7 were controls.
    Summary · context
    For microsurgery, the lateral corticospinal tract T10 was grafted to the denervated internal obliquus abdominal muscle.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    14 Oct 2026, 05:02 UTC

    Study results updated

  2. Version 1

    14 Oct 2026, 01:46 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Keywords

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