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Study · Not a test of the peptide alone · 2020

5 results

High yield expression, characterization, and biological activity of IFNα2-Tα1 fusion protein.

Aslam MS, Gull I, Mahmood MS, Iqbal MM, Abbas Z, Tipu I, Ahmed A, Athar MA

Preparative biochemistry & biotechnology13 Nov 2019Cited by 3 (Europe PMC)

PMID31718419 (opens in a new tab)DOI10.1080/10826068.2019.1689509 (opens in a new tab)PepBankPUB-31718419
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone5

In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin alpha-1.

  1. 01LabNot a test of Thymosin alpha-1 aloneOther designOther

    The IFNα2-Tα1 fusion protein expressed in Escherichia coli reached maximum expression in modified terrific broth medium with lactose as inducer.

    In plain wordsIn lab production, a fusion of interferon and thymosin alpha‑1 was made best in modified broth with lactose.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableFusion protein expression level
    Study

    n: not reported in source

    Outcome group
    Synthesis and manufacturing · Pharmacology

    Source sentences

    Summary
    The expression of IFNα2-Tα1 fusion protein was optimized to higher level and its maximum expression was obtained in modified terrific broth medium when lactose was used as inducer.
    2 context sentences
    Summary · context
    In this study we produced an important human interferon α-2-thymosin α-1 (IFNα2-Tα1) fusion protein with probable pharmaceutical properties coupled to its high-level expression, characterization, and study of its biological activity.
    Summary · context
    The IFNα2-Tα1 fusion gene was constructed by over-lap extension PCR and expressed in Escherichia coli expression system.

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

  2. 02LabNot a test of Thymosin alpha-1 aloneOther designOther

    The IFNα2-Tα1 fusion protein was refolded into its native active form with a maximum yield of 83.14%.

    In plain wordsIn lab production, the interferon and thymosin alpha‑1 fusion protein was refolded to its active form with high yield.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableRefolding yield
    Study

    n: not reported in source

    Outcome group
    Synthesis and manufacturing · Pharmacology

    Measurements

    Refolding yield
    83.14%
    IFNα2-Tα1 fusion protein

    Source sentences

    Summary
    The fusion protein was refolded into its native biologically active form with maximum yield of 83.14%
    2 context sentences
    Summary · context
    In this study we produced an important human interferon α-2-thymosin α-1 (IFNα2-Tα1) fusion protein with probable pharmaceutical properties coupled to its high-level expression, characterization, and study of its biological activity.
    Summary · context
    The IFNα2-Tα1 fusion gene was constructed by over-lap extension PCR and expressed in Escherichia coli expression system.

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

  3. 03LabNot a test of Thymosin alpha-1 aloneOther designOther

    Purification of the IFNα2-Tα1 fusion protein gave about 98% purity and 69% final yield.

    In plain wordsIn lab production, purification gave a fusion protein of high purity.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicablePurity and final yield after purification
    Study

    n: not reported in source

    Outcome group
    Synthesis and manufacturing · Pharmacology

    Measurements

    Purity
    ≈ 98%
    IFNα2-Tα1 fusion protein
    Final yield
    69%
    IFNα2-Tα1 fusion protein

    Source sentences

    Summary
    followed by purification with ∼98% purity and 69% final yield.
    2 context sentences
    Summary · context
    In this study we produced an important human interferon α-2-thymosin α-1 (IFNα2-Tα1) fusion protein with probable pharmaceutical properties coupled to its high-level expression, characterization, and study of its biological activity.
    Summary · context
    The IFNα2-Tα1 fusion gene was constructed by over-lap extension PCR and expressed in Escherichia coli expression system.

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

  4. 04LabNot a test of Thymosin alpha-1 aloneOther designActivity measurement

    The IFNα2-Tα1 fusion protein showed both IFNα2 and Tα1 biological activities.

    In plain wordsIn lab tests, the fusion protein kept the activities of both interferon and thymosin alpha‑1.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableIFNα2 and Tα1 activities of fusion protein
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    it exhibits both IFNα2 & Tα1 activities
    2 context sentences
    Summary · context
    In this study we produced an important human interferon α-2-thymosin α-1 (IFNα2-Tα1) fusion protein with probable pharmaceutical properties coupled to its high-level expression, characterization, and study of its biological activity.
    Summary · context
    The IFNα2-Tα1 fusion gene was constructed by over-lap extension PCR and expressed in Escherichia coli expression system.

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

  5. 05LabNot a test of Thymosin alpha-1 aloneOther designActivity measurement

    The IFNα2-Tα1 fusion protein had significantly higher anticancer activity than IFNα-2 alone.

    In plain wordsIn lab tests, the fusion protein had stronger anticancer activity than interferon alone.

    Peptide
    Thymosin alpha-1
    Outcome
    IncreaseAnticancer activity versus IFNα-2 alone
    Study

    n: not reported in source

    Comparator
    IFNα-2 alone
    Outcome group
    Tumor growth · Cancer

    Source sentences

    Summary
    significantly higher anticancer activity as compared to IFNα-2 alone.
    2 context sentences
    Summary · context
    In this study we produced an important human interferon α-2-thymosin α-1 (IFNα2-Tα1) fusion protein with probable pharmaceutical properties coupled to its high-level expression, characterization, and study of its biological activity.
    Summary · context
    The IFNα2-Tα1 fusion gene was constructed by over-lap extension PCR and expressed in Escherichia coli expression system.

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    10 Oct 2026, 08:55 UTC

    Study results updated

  2. Version 2

    9 Oct 2026, 03:03 UTC

    Study data updated · source update from Europe PMC

  3. Version 1

    9 Oct 2026, 03:02 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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