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Study · Animal study (in vivo) · mouse and rat · 2019

13 results

Cerebrolysin Ameliorates Focal Cerebral Ischemia Injury Through Neuroinflammatory Inhibition via CREB/PGC-1α Pathway.

Guan X, Wang Y, Kai G, Zhao S, Huang T, Li Y, Xu Y, Zhang L, Pang T

Frontiers in pharmacology22 Oct 2019Cited by 59 (Europe PMC)

PMID31695614 (opens in a new tab)PMCIDPMC6818051 (opens in a new tab)DOI10.3389/fphar.2019.01245 (opens in a new tab)PepBankPUB-31695614
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Summary

Neuroinflammation is one of the important factors aggravating brain injury after ischemic stroke. We aimed to investigate the effects of cerebrolysin (CBL) on neuroinflammation in vivo and in vitro and the underlying mechanisms. The gene expressions of pro-inflammatory factors and anti-inflammatory factors were analyzed by real time PCR in rat transient middle cerebral artery occlusion (tMCAO) model, lipopolysaccharides-induced neuroinflammatory mice model and LPS-treated mouse primary microglia cells. The neuroprotective effects of CBL were evaluated by infarct size, Longa test and Rotarod test for long-term functional recovery in rats subjected to ischemia. The role of CREB/PGC-1α pathway in anti-neuroinflammatory effect of CBL was also determined by real time PCR and Western blotting. In the tMCAO model, administration of CBL at 3 h post-ischemia reduced infarct volume, promoted long-term functional recovery, decreased the gene expression of pro-inflammatory factors and increased the gene expression of anti-inflammatory factors. Correspondingly, in LPS-induced neuroinflammatory mice model, CBL treatment attenuated sickness behavior, decreased the gene expression of pro-inflammatory factors, and increased the gene expression of anti-inflammatory factors. In in vitro and in vivo experiments, CBL increased the protein expression levels of PGC-1α and phosphorylated CREB to play anti-inflammatory effect. Additionally, the application of the specific CREB inhibitor, 666-15 compound could effectively reverse the anti-inflammatory effect of CBL in primary mouse microglia cells and anti-ischemic brain injury of CBL in rats subjected to tMCAO. In conclusion, CBL ameliorated cerebral ischemia injury through reducing neuroinflammation partly via the activation of CREB/PGC-1α pathway and may play a therapeutic role as anti-neuroinflammatory agents in the brain disorders associated with neuroinflammation.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study13

11 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In rats subjected to transient middle cerebral artery occlusion, Cerebrolysin administered post-ischemia reduced infarct volume.

    In plain wordsIn rats with cerebral ischemia, Cerebrolysin reduced the volume of damaged brain tissue.

    Peptide
    Cerebrolysin
    Outcome
    DecreaseInfarct volume
    Study

    ratn: not reported in source

    transient middle cerebral artery occlusion
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In the tMCAO model, administration of CBL at 3 h post-ischemia reduced infarct volume, promoted long-term functional recovery, decreased the gene expression of pro-inflammatory factors and increased the gene expression of anti-inflammatory factors.
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In rats subjected to transient middle cerebral artery occlusion, Cerebrolysin promoted long-term functional recovery.

    In plain wordsIn rats with cerebral ischemia, Cerebrolysin improved long-term functional recovery.

    Peptide
    Cerebrolysin
    Outcome
    IncreaseLong-term functional recovery
    Study

    ratn: not reported in source

    transient middle cerebral artery occlusion
    Outcome group
    Exercise and physical function · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In the tMCAO model, administration of CBL at 3 h post-ischemia reduced infarct volume, promoted long-term functional recovery, decreased the gene expression of pro-inflammatory factors and increased the gene expression of anti-inflammatory factors.
  3. 03AnimalAnimal study (in vivo)Mechanism

    In rats subjected to transient middle cerebral artery occlusion, Cerebrolysin decreased pro-inflammatory factor gene expression.

    In plain wordsIn rats with cerebral ischemia, Cerebrolysin reduced gene expression of pro-inflammatory factors.

    Peptide
    Cerebrolysin
    Outcome
    DecreasePro-inflammatory gene expression
    Study

    ratn: not reported in source

    transient middle cerebral artery occlusion
    Outcome group
    Inflammation markers · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In the tMCAO model, administration of CBL at 3 h post-ischemia reduced infarct volume, promoted long-term functional recovery, decreased the gene expression of pro-inflammatory factors and increased the gene expression of anti-inflammatory factors.
  4. 04AnimalAnimal study (in vivo)Mechanism

    In rats subjected to transient middle cerebral artery occlusion, Cerebrolysin increased anti-inflammatory factor gene expression.

    In plain wordsIn rats with cerebral ischemia, Cerebrolysin increased gene expression of anti-inflammatory factors.

    Peptide
    Cerebrolysin
    Outcome
    IncreaseAnti-inflammatory gene expression
    Study

    ratn: not reported in source

    transient middle cerebral artery occlusion
    Outcome group
    Inflammation markers · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In the tMCAO model, administration of CBL at 3 h post-ischemia reduced infarct volume, promoted long-term functional recovery, decreased the gene expression of pro-inflammatory factors and increased the gene expression of anti-inflammatory factors.
  5. 05AnimalAnimal study (in vivo)Efficacy outcome

    In mice with LPS-induced neuroinflammation, Cerebrolysin attenuated sickness behavior.

    In plain wordsIn mice with induced brain inflammation, Cerebrolysin reduced sickness behavior.

    Peptide
    Cerebrolysin
    Outcome
    DecreaseSickness behavior
    Study

    mousen: not reported in source

    LPS-induced neuroinflammation
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Correspondingly, in LPS-induced neuroinflammatory mice model, CBL treatment attenuated sickness behavior, decreased the gene expression of pro-inflammatory factors, and increased the gene expression of anti-inflammatory factors.
  6. 06AnimalAnimal study (in vivo)Mechanism

    In mice with LPS-induced neuroinflammation, Cerebrolysin decreased pro-inflammatory factor gene expression.

    In plain wordsIn mice with induced brain inflammation, Cerebrolysin reduced gene expression of pro-inflammatory factors.

    Peptide
    Cerebrolysin
    Outcome
    DecreasePro-inflammatory gene expression
    Study

    mousen: not reported in source

    LPS-induced neuroinflammation
    Outcome group
    Inflammation markers · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Correspondingly, in LPS-induced neuroinflammatory mice model, CBL treatment attenuated sickness behavior, decreased the gene expression of pro-inflammatory factors, and increased the gene expression of anti-inflammatory factors.
  7. 07AnimalAnimal study (in vivo)Mechanism

    In mice with LPS-induced neuroinflammation, Cerebrolysin increased anti-inflammatory factor gene expression.

    In plain wordsIn mice with induced brain inflammation, Cerebrolysin increased gene expression of anti-inflammatory factors.

    Peptide
    Cerebrolysin
    Outcome
    IncreaseAnti-inflammatory gene expression
    Study

    mousen: not reported in source

    LPS-induced neuroinflammation
    Outcome group
    Inflammation markers · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Correspondingly, in LPS-induced neuroinflammatory mice model, CBL treatment attenuated sickness behavior, decreased the gene expression of pro-inflammatory factors, and increased the gene expression of anti-inflammatory factors.
  8. 08AnimalAnimal study (in vivo)Mechanism

    In animal experiments, Cerebrolysin increased PGC-1α protein expression.

    In plain wordsIn animals, Cerebrolysin increased PGC-1α protein expression.

    Peptide
    Cerebrolysin
    Outcome
    IncreasePGC-1α protein expression
    Study

    n: not reported in source

    in vivo neuroinflammation experiments
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In in vitro and in vivo experiments, CBL increased the protein expression levels of PGC-1α and phosphorylated CREB to play anti-inflammatory effect.
    1 context sentence
    Summary · context
    The gene expressions of pro-inflammatory factors and anti-inflammatory factors were analyzed by real time PCR in rat transient middle cerebral artery occlusion (tMCAO) model, lipopolysaccharides-induced neuroinflammatory mice model and LPS-treated mouse primary microglia cells.
  9. 09AnimalAnimal study (in vivo)Mechanism

    In animal experiments, Cerebrolysin increased phosphorylated CREB protein expression.

    In plain wordsIn animals, Cerebrolysin increased phosphorylated CREB protein expression.

    Peptide
    Cerebrolysin
    Outcome
    IncreasePhosphorylated CREB protein expression
    Study

    n: not reported in source

    in vivo neuroinflammation experiments
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In in vitro and in vivo experiments, CBL increased the protein expression levels of PGC-1α and phosphorylated CREB to play anti-inflammatory effect.
    1 context sentence
    Summary · context
    The gene expressions of pro-inflammatory factors and anti-inflammatory factors were analyzed by real time PCR in rat transient middle cerebral artery occlusion (tMCAO) model, lipopolysaccharides-induced neuroinflammatory mice model and LPS-treated mouse primary microglia cells.
  10. 10LabIn vitro (cells)Mechanism

    In mouse primary microglia cells, Cerebrolysin increased PGC-1α protein expression.

    In plain wordsIn lab tests, Cerebrolysin increased PGC-1α protein expression.

    Peptide
    Cerebrolysin
    Outcome
    IncreasePGC-1α protein expression
    Study

    mousen: not reported in source

    primary microglia
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In in vitro and in vivo experiments, CBL increased the protein expression levels of PGC-1α and phosphorylated CREB to play anti-inflammatory effect.
    1 context sentence
    Summary · context
    The gene expressions of pro-inflammatory factors and anti-inflammatory factors were analyzed by real time PCR in rat transient middle cerebral artery occlusion (tMCAO) model, lipopolysaccharides-induced neuroinflammatory mice model and LPS-treated mouse primary microglia cells.
  11. 11LabIn vitro (cells)Mechanism

    In mouse primary microglia cells, Cerebrolysin increased phosphorylated CREB protein expression.

    In plain wordsIn lab tests, Cerebrolysin increased phosphorylated CREB protein expression.

    Peptide
    Cerebrolysin
    Outcome
    IncreasePhosphorylated CREB protein expression
    Study

    mousen: not reported in source

    primary microglia
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In in vitro and in vivo experiments, CBL increased the protein expression levels of PGC-1α and phosphorylated CREB to play anti-inflammatory effect.
    1 context sentence
    Summary · context
    The gene expressions of pro-inflammatory factors and anti-inflammatory factors were analyzed by real time PCR in rat transient middle cerebral artery occlusion (tMCAO) model, lipopolysaccharides-induced neuroinflammatory mice model and LPS-treated mouse primary microglia cells.

Studies that did not test the peptide alone2

In this study, Cerebrolysin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Cerebrolysin.

  1. 12AnimalNot a test of Cerebrolysin aloneAnimal study (in vivo)Mechanism

    In rats subjected to transient middle cerebral artery occlusion, the CREB inhibitor reversed the anti-ischemic brain effect of Cerebrolysin.

    In plain wordsIn rats with cerebral ischemia, a CREB inhibitor reversed Cerebrolysin's protection against brain injury.

    Peptide
    Cerebrolysin
    Outcome
    DecreaseReversal of anti-ischemic brain protection
    Study

    ratn: not reported in source

    transient middle cerebral artery occlusion
    Outcome group
    Neuroprotection · Nervous system and cognition

    Source sentences

    Summary
    Additionally, the application of the specific CREB inhibitor, 666-15 compound could effectively reverse the anti-inflammatory effect of CBL in primary mouse microglia cells and anti-ischemic brain injury of CBL in rats subjected to tMCAO.

    In this study, Cerebrolysin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Cerebrolysin.

  2. 13LabNot a test of Cerebrolysin aloneIn vitro (cells)Mechanism

    In primary mouse microglia cells, the CREB inhibitor reversed the anti-inflammatory effect of Cerebrolysin.

    In plain wordsIn cells from mice, a CREB inhibitor reversed Cerebrolysin's anti-inflammatory effect.

    Peptide
    Cerebrolysin
    Outcome
    DecreaseReversal of anti-inflammatory effect
    Study

    mousen: not reported in source

    primary microglia
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition

    Source sentences

    Summary
    Additionally, the application of the specific CREB inhibitor, 666-15 compound could effectively reverse the anti-inflammatory effect of CBL in primary mouse microglia cells and anti-ischemic brain injury of CBL in rats subjected to tMCAO.

    In this study, Cerebrolysin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Cerebrolysin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    14 Oct 2026, 05:02 UTC

    Study results updated

  2. Version 1

    14 Oct 2026, 01:46 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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