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Study · Measured the body's own peptide · 2019

1 result

Short-term creatine supplementation changes protein metabolism signaling in hindlimb suspension.

Marzuca-Nassr GN, Fortes MAS, Guimarães-Ferreira L, Murata GM, Vitzel KF, Vasconcelos DAA, Bassit RA, Curi R

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas7 Oct 2019Cited by 13 (Europe PMC)

PMID31596311 (opens in a new tab)PMCIDPMC6787955 (opens in a new tab)DOI10.1590/1414-431x20198391 (opens in a new tab)PepBankPUB-31596311
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Summary

The effect of a short-term creatine supplementation on hindlimb suspension (HS)-induced muscle atrophy was investigated. Creatine monohydrate (5 g/kg b.w. per day) or placebo, divided in 2 daily doses, was given by oral gavage for 5 days. Rats were maintained in HS with dietary supplementation concomitantly for 5 days. Body weight, soleus and EDL muscle masses, and cross-sectional areas (CSA) of the muscle fibers were measured. Signaling pathways associated with skeletal muscle mass regulation (FST, MSTN, FAK, IGF-1, MGF, Akt, mTOR, atrogin-1, and MuRF1 expressions, and Akt, S6, GSK3B, and 4EBP1 proteins) were evaluated in the muscles. Soleus muscle exhibited more atrophy than the EDL muscle due to HS. Creatine supplementation attenuated the decrease of wet weight and increased p-4EBP1 protein in the EDL muscle of HS rats. Also, creatine increased mTOR and atrogin-1 expressions in the same muscle and condition. In the absence of HS, creatine supplementation increased FAK and decreased MGF expressions in the EDL muscle. Creatine attenuated the increase in FST expression due to HS in the soleus muscle. MuRF1 expression increased in the soleus muscle due to creatine supplementation in HS animals whereas atrogin-1 expression increased still further in this group compared with untreated HS rats. In conclusion, short-term creatine supplementation changed protein metabolism signaling in soleus and EDL muscles. However, creatine supplementation only slightly attenuated the mass loss of both muscles and did not prevent the CSA reduction and muscle strength decrease induced by HS for 5 days.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study1

No result from this study comes from a test of the peptide.

Natural levels measured1

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

  1. 01AnimalEndogenous levelAnimal study (in vivo)Mechanism

    In rats without hindlimb suspension, creatine supplementation decreased the body's own MGF expression in the EDL muscle.

    In plain wordsIn rats, creatine lowered the body's own MGF expression in one leg muscle when the rats were not suspended.

    Peptide
    MGF / PEG-MGF
    Outcome
    DecreaseMGF expression in EDL muscle without hindlimb suspension
    Study

    ratn: not reported in source

    EDL muscle
    Outcome group
    Muscle injury and repair · Musculoskeletal

    Source sentences

    Summary
    In the absence of HS, creatine supplementation increased FAK and decreased MGF expressions in the EDL muscle.
    2 context sentences
    Summary · context
    Creatine monohydrate (5 g/kg b.w. per day) or placebo, divided in 2 daily doses, was given by oral gavage for 5 days.
    Summary · context
    Rats were maintained in HS with dietary supplementation concomitantly for 5 days.

    This result measures the body's own MGF / PEG-MGF. Nobody was given the peptide. It is not evidence about taking it.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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