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Study · Not a test of the peptide alone · 2019

2 results

Potential role of exercise-induced glucose-6-phosphate isomerase in skeletal muscle function.

Kwak SE, Shin HE, Zhang DD, Lee J, Yoon KJ, Bae JH, Moon HY, Song W

Journal of exercise nutrition & biochemistry1 Jun 2019Cited by 5 (Europe PMC)

PMID31337203 (opens in a new tab)PMCIDPMC6651688 (opens in a new tab)DOI10.20463/jenb.2019.0014 (opens in a new tab)PepBankPUB-31337203
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Summary

PurposeRecent studies have shown that glucose-6-phosphate isomerase (GPI)-which is a glycolysis interconversion enzyme-reduces oxidative stress. However, these studies are limited to tumors such as fibrosarcoma, and there are no studies that have examined the effects of exercise on GPI expression in mice skeletal muscle. Furthermore, GPI acts in an autocrine manner thorough its receptor, autocrine motility factor receptor (AMFR); therefore, we investigated expression level changes of secreted GPI from skeletal muscle in in vitro study to examine the potential role of GPI on skeletal muscle.MethodsFirst, we performed an in vitro study, to identify the condition that upregulates GPI levels in skeletal muscle cells; we treated C2C12 muscle cells with an exercise-mimicking chemical, AICAR. AICAR treatment upregulated GPI expression level in C2C12 cell and its secretomes. To confirm the direct effect of GPI on skeletal muscle cells, we treated C2C12 cells with GPI recombinant protein.ResultsWe found that GPI improved the viability of C2C12 cells. In the in vivo study, the exercise-treated mice group showed upregulated GPI expression in skeletal muscle. Based on the in vitro study results, we speculated that expression level of GPI in skeletal muscle might be associated with muscle function. We analyzed the association between GPI expression level and the grip strength of the all mice group. The mice group's grip strengths were upregulated after 2 weeks of treadmill exercise, and GPI expression level positively correlated with the grip strength.ConclusionThese results suggested that the exercise-induced GPI expression in skeletal muscle might have a positive effect on skeletal muscle function.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study2

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone2

In this study, AICAR (acadesine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking AICAR (acadesine).

  1. 01LabNot a test of AICAR (acadesine) aloneIn vitro (cells)Mechanism

    In C2C12 muscle cells, AICAR increased GPI expression.

    In plain wordsIn cells, AICAR increased GPI expression.

    Peptide
    AICAR (acadesine)
    Outcome
    IncreaseCellular GPI expression
    Study

    mousen: not reported in source

    C2C12 muscle cells
    Outcome group
    Receptor activity and mechanism · Musculoskeletal

    Source sentences

    Summary
    AICAR treatment upregulated GPI expression level in C2C12 cell and its secretomes.
    1 context sentence
    Summary · context
    First, we performed an in vitro study, to identify the condition that upregulates GPI levels in skeletal muscle cells; we treated C2C12 muscle cells with an exercise-mimicking chemical, AICAR.

    In this study, AICAR (acadesine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking AICAR (acadesine).

  2. 02LabNot a test of AICAR (acadesine) aloneIn vitro (cells)Mechanism

    In C2C12 muscle cell secretomes, AICAR increased GPI expression.

    In plain wordsIn cells, AICAR increased GPI in secreted material.

    Peptide
    AICAR (acadesine)
    Outcome
    IncreaseSecretome GPI expression
    Study

    mousen: not reported in source

    C2C12 muscle cells
    Outcome group
    Receptor activity and mechanism · Musculoskeletal

    Source sentences

    Summary
    AICAR treatment upregulated GPI expression level in C2C12 cell and its secretomes.
    1 context sentence
    Summary · context
    First, we performed an in vitro study, to identify the condition that upregulates GPI levels in skeletal muscle cells; we treated C2C12 muscle cells with an exercise-mimicking chemical, AICAR.

    In this study, AICAR (acadesine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking AICAR (acadesine).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Keywords

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