Local Vibration Stimuli Induce Mechanical Stress-Induced Factors and Facilitate Recovery From Immobilization-Induced Oxidative Myofiber Atrophy in Rats.

Usuki F, Fujimura M, Nakamura A, Nakano J, Okita M, Higuchi I

Frontiers in physiology20 Jun 2019Cited by 10 (Europe PMC)

PMID31281262PMCIDPMC6595229DOI10.3389/fphys.2019.00759PepBankPUB-31281262

Summary

Muscle atrophy can be caused by unloading stress such as microgravity environments or cast immobilization. Therapies for such disuse muscle atrophy and their underlying mechanisms are incompletely understood. Here, we investigated the therapeutic effects of local vibration stimulation on immobilization-induced skeletal muscle atrophy. A rat model was made by placing the left hindlimb in a cast for 1 week, leading to oxidative myofiber atrophy without myopathic changes in soleus skeletal muscle. Vibration stimulus (90 Hz, 15 min) to the plantar fascia of the atrophic hindlimb was performed once a day using a hand-held vibration massager after removal of a cast at the end of the immobilization period. After 2 weeks, rats were dissected, and quantitative analysis of the cross-sectional areas of soleus myofibers was performed. The results revealed that vibration induced significant recovery from disuse muscle atrophy, compared with untreated immobilized samples. Furthermore, vibration treatment suppressed the fiber transition from slow to fast fiber types compared with vibration-untreated immobilized samples. Western blotting analyses of mechanical stress-induced factors revealed that the expression of mechano-growth factor (MGF), systemic insulin-like growth factor I, and the mechanotransduction protein, Yes-associated protein 1 (YAP1), was decreased in untreated immobilized soleus muscle, whereas vibration stimulation restored their expression. No change in the level of phosphorylation of YAP1^Ser127 was observed, leading to no change in p-YAP1/YAP1 ratio in vibration-treated immobilized soleus muscle. The results indicate that vibration stimulus is effective to restore immobilization-induced inactivation of YAP1 pathway. Phosphorylation of ERK 1/2, but not AKT, was enhanced in vibration-treated immobilized soleus muscle. Furthermore, vibration stimuli restored immobilization-induced downregulation of the paired box transcription factor, PAX7, a critical factor for regenerative myogenesis in muscle satellite cells. Our results indicate that cyclic vibration stimuli are effective in activating satellite cells and facilitate recovery from immobilization-induced oxidative myofiber atrophy through upregulation of MGF and YAP1.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study2

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01AnimalEndogenous levelAnimal study (in vivo)Mechanism
In rats, MGF expression was decreased in untreated immobilized soleus muscle.
In plain wordsIn rats, a leg kept in a cast had less of the muscle's own MGF.
Peptide
MGF / PEG-MGF
Outcome
DecreaseMGF expression in immobilized soleus muscle
Study
ratn: not reported in source
Hindlimb cast immobilization with soleus muscle atrophy
Outcome group
Endogenous peptide levels · Musculoskeletal
Source sentences
Summary
Western blotting analyses of mechanical stress-induced factors revealed that the expression of mechano-growth factor (MGF), systemic insulin-like growth factor I, and the mechanotransduction protein, Yes-associated protein 1 (YAP1), was decreased in untreated immobilized soleus muscle, whereas vibration stimulation restored their expression.
1 context sentence(design, population, supporting numbers)
Summary · context
A rat model was made by placing the left hindlimb in a cast for 1 week, leading to oxidative myofiber atrophy without myopathic changes in soleus skeletal muscle.
This result measures the body's own MGF / PEG-MGF. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-3128126201
02AnimalEndogenous levelAnimal study (in vivo)Mechanism
In rats with immobilized hindlimbs, local vibration restored MGF expression in soleus muscle compared with untreated immobilized muscle.
In plain wordsIn rats with a casted leg, vibration brought back the muscle's own MGF level.
Peptide
MGF / PEG-MGF
Outcome
IncreaseMGF expression after vibration in immobilized soleus muscle
Study
ratn: not reported in source
Hindlimb cast immobilization with soleus muscle atrophy
Comparator
Untreated immobilized soleus muscle
Outcome group
Endogenous peptide levels · Musculoskeletal
Source sentences
Summary
Western blotting analyses of mechanical stress-induced factors revealed that the expression of mechano-growth factor (MGF), systemic insulin-like growth factor I, and the mechanotransduction protein, Yes-associated protein 1 (YAP1), was decreased in untreated immobilized soleus muscle, whereas vibration stimulation restored their expression.
1 context sentence(design, population, supporting numbers)
Summary · context
A rat model was made by placing the left hindlimb in a cast for 1 week, leading to oxidative myofiber atrophy without myopathic changes in soleus skeletal muscle.
This result measures the body's own MGF / PEG-MGF. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-3128126202

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Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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