[Mechano-growth factor regulated cyclic stretch-induced osteogenic differentiation and MMP-1, MMP-2 expression in human periodontal ligament cells by activating the MEK/ERK1/2 pathway].

Chen JT, Wang Y, Zhou ZF, Wei KW

Shanghai kou qiang yi xue = Shanghai journal of stomatology1 Feb 2019

PMID31080992PepBankPUB-31080992

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study9

3 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In human periodontal ligament cells, MGF stimulation enhanced the effect of cyclic stretch on osteogenic differentiation.
In plain wordsIn lab-grown gum ligament cells, adding MGF increased the bone-forming effect of stretch, compared with stretch alone.
Peptide
MGF / PEG-MGF
Outcome
IncreaseOsteogenic differentiation with MGF stimulation
Study
humann: not reported in source
Human periodontal ligament cells under cyclic stretch
Comparator
Cyclic stretch group
Outcome group
Bone and joint · Musculoskeletal
Studied dose
Dose not stated
Source sentences
Summary
stimulation with MGF further enhanced the effects of CS on osteogenic differentiation of hPDLCs and MMP-1, MMP-2 expression (P＜0.05).
2 context sentences(design, population, supporting numbers)
Summary · context
HPDLCs were isolated and transfected with si-MGF, or stimulated with MGF or MEK/ERK pathway inhibitor U0126.
Summary · context
Cells were cultured in Flexercell system with 10% elongation at 0.1 Hz.
Report a problemFND-3108099205
02LabIn vitro (cells)Mechanism
In human periodontal ligament cells, MGF stimulation enhanced the effect of cyclic stretch on MMP-1 and MMP-2 expression.
In plain wordsIn lab-grown gum ligament cells, adding MGF raised MMP-1 and MMP-2 more than stretch alone.
Peptide
MGF / PEG-MGF
Outcome
IncreaseMMP-1 and MMP-2 expression with MGF stimulation
Study
humann: not reported in source
Human periodontal ligament cells under cyclic stretch
Comparator
Cyclic stretch group
Outcome group
Inflammation markers · Musculoskeletal
Studied dose
Dose not stated
Source sentences
Summary
Conversely, stimulation with MGF further enhanced the effects of CS on osteogenic differentiation of hPDLCs and MMP-1, MMP-2 expression (P＜0.05).
2 context sentences(design, population, supporting numbers)
Summary · context
HPDLCs were isolated and transfected with si-MGF, or stimulated with MGF or MEK/ERK pathway inhibitor U0126.
Summary · context
Cells were cultured in Flexercell system with 10% elongation at 0.1 Hz.
Report a problemFND-3108099206
03LabIn vitro (cells)Mechanism
In human periodontal ligament cells, MGF treatment further enhanced the cyclic stretch-induced expression of p-ERK.
In plain wordsIn lab-grown gum ligament cells, adding MGF raised the stretch-driven p-ERK level further.
Peptide
MGF / PEG-MGF
Outcome
Increasep-ERK expression with MGF treatment
Study
humann: not reported in source
Human periodontal ligament cells under cyclic stretch
Comparator
Cyclic stretch group
Outcome group
Receptor activity and mechanism · Musculoskeletal
Studied dose
Dose not stated
Source sentences
Summary
Additionally, MGC silencing abrogated CS-induced expression of p-ERK (P＜0.05), which was further enhanced following MGF treatment (P＜0.05).
2 context sentences(design, population, supporting numbers)
Summary · context
HPDLCs were isolated and transfected with si-MGF, or stimulated with MGF or MEK/ERK pathway inhibitor U0126.
Summary · context
Cells were cultured in Flexercell system with 10% elongation at 0.1 Hz.
Report a problemFND-3108099208

Natural levels measured1

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

04LabEndogenous levelIn vitro (cells)Mechanism
In human periodontal ligament cells, cyclic stretch increased the cells' own MGF expression over time.
In plain wordsIn lab-grown gum ligament cells, stretching raised the cells' own MGF over time.
Peptide
MGF / PEG-MGF
Outcome
IncreaseMGF expression under cyclic stretch
Study
humann: not reported in source
Human periodontal ligament cells under cyclic stretch
Comparator
Control
Outcome group
Receptor activity and mechanism · Musculoskeletal
Source sentences
Summary
CS induced the expression of MGF in hPDLCs in a time-dependent manner (P＜0.05).
2 context sentences(design, population, supporting numbers)
Summary · context
HPDLCs were isolated and transfected with si-MGF, or stimulated with MGF or MEK/ERK pathway inhibitor U0126.
Summary · context
Cells were cultured in Flexercell system with 10% elongation at 0.1 Hz.
This result measures the body's own MGF / PEG-MGF. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3108099201

Studies that did not test the peptide alone5

In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking MGF / PEG-MGF.

05LabNot a test of MGF / PEG-MGF aloneIn vitro (cells)Mechanism
In human periodontal ligament cells, si-MGF knockdown of MGF suppressed ALP activity.
In plain wordsIn lab-grown gum ligament cells, switching off the MGF gene lowered a bone-forming marker (ALP activity).
Peptide
MGF / PEG-MGF
Outcome
DecreaseALP activity after MGF knockdown
Study
humann: not reported in source
Human periodontal ligament cells under cyclic stretch
Outcome group
Bone and joint · Musculoskeletal
Source sentences
Summary
Moreover, cessation of MGF dramatically suppressed ALP activity (P＜0.05) and the expression of osteogenic gene ALP, Runx2 and OPN (P＜0.05) in hPDLCs.
2 context sentences(design, population, supporting numbers)
Summary · context
HPDLCs were isolated and transfected with si-MGF, or stimulated with MGF or MEK/ERK pathway inhibitor U0126.
Summary · context
Cells were cultured in Flexercell system with 10% elongation at 0.1 Hz.
In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MGF / PEG-MGF.
Report a problemFND-3108099202
06LabNot a test of MGF / PEG-MGF aloneIn vitro (cells)Mechanism
In human periodontal ligament cells, si-MGF knockdown of MGF suppressed expression of the osteogenic genes ALP, Runx2 and OPN.
In plain wordsIn lab-grown gum ligament cells, switching off the MGF gene lowered the activity of three bone-forming genes.
Peptide
MGF / PEG-MGF
Outcome
DecreaseOsteogenic gene expression after MGF knockdown
Study
humann: not reported in source
Human periodontal ligament cells under cyclic stretch
Outcome group
Bone and joint · Musculoskeletal
Source sentences
Summary
Moreover, cessation of MGF dramatically suppressed ALP activity (P＜0.05) and the expression of osteogenic gene ALP, Runx2 and OPN (P＜0.05) in hPDLCs.
2 context sentences(design, population, supporting numbers)
Summary · context
HPDLCs were isolated and transfected with si-MGF, or stimulated with MGF or MEK/ERK pathway inhibitor U0126.
Summary · context
Cells were cultured in Flexercell system with 10% elongation at 0.1 Hz.
In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MGF / PEG-MGF.
Report a problemFND-3108099203
07LabNot a test of MGF / PEG-MGF aloneIn vitro (cells)Mechanism
In human periodontal ligament cells, down-regulation of MGF restrained MMP-1 and MMP-2 expression compared with the cyclic stretch group.
In plain wordsIn lab-grown gum ligament cells, switching off the MGF gene lowered MMP-1 and MMP-2, compared with stretch alone.
Peptide
MGF / PEG-MGF
Outcome
DecreaseMMP-1 and MMP-2 expression after MGF knockdown
Study
humann: not reported in source
Human periodontal ligament cells under cyclic stretch
Comparator
Cyclic stretch group
Outcome group
Inflammation markers · Musculoskeletal
Source sentences
Summary
Furthermore, down-regulation of MGF restrained the expression of MMP-1 and MMP-2, in contrast to CS group (P＜0.05).
2 context sentences(design, population, supporting numbers)
Summary · context
HPDLCs were isolated and transfected with si-MGF, or stimulated with MGF or MEK/ERK pathway inhibitor U0126.
Summary · context
Cells were cultured in Flexercell system with 10% elongation at 0.1 Hz.
In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MGF / PEG-MGF.
Report a problemFND-3108099204
08LabNot a test of MGF / PEG-MGF aloneIn vitro (cells)Mechanism
In human periodontal ligament cells, MGF silencing abrogated the cyclic stretch-induced expression of p-ERK.
In plain wordsIn lab-grown gum ligament cells, switching off the MGF gene blocked the stretch-driven rise in p-ERK.
Peptide
MGF / PEG-MGF
Outcome
Decreasep-ERK expression after MGF silencing
Study
humann: not reported in source
Human periodontal ligament cells under cyclic stretch
Comparator
Cyclic stretch group
Outcome group
Receptor activity and mechanism · Musculoskeletal
Source sentences
Summary
MGC silencing abrogated CS-induced expression of p-ERK
2 context sentences(design, population, supporting numbers)
Summary · context
HPDLCs were isolated and transfected with si-MGF, or stimulated with MGF or MEK/ERK pathway inhibitor U0126.
Summary · context
Cells were cultured in Flexercell system with 10% elongation at 0.1 Hz.
In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MGF / PEG-MGF.
Report a problemFND-3108099207
09LabNot a test of MGF / PEG-MGF aloneIn vitro (cells)Mechanism
In human periodontal ligament cells, the MEK/ERK inhibitor U0126 antagonized the MGF-enhanced effects of cyclic stretch on osteogenic differentiation and MMP-1 and MMP-2 expression.
In plain wordsIn lab-grown gum ligament cells, the MEK/ERK blocker U0126 cancelled the added effects of MGF on stretch.
Peptide
MGF / PEG-MGF
Outcome
DecreaseMGF-enhanced osteogenic differentiation and MMP expression with U0126
Study
humann: not reported in source
Human periodontal ligament cells under cyclic stretch
Comparator
MGF with cyclic stretch without U0126
Outcome group
Receptor activity and mechanism · Musculoskeletal
Source sentences
Summary
Simultaneously, precondition with U0126 antagonized MGF-enhanced effects on CS-triggered osteogenic differentiation and MMP-1, MMP-2 expression (P＜0.05).
2 context sentences(design, population, supporting numbers)
Summary · context
HPDLCs were isolated and transfected with si-MGF, or stimulated with MGF or MEK/ERK pathway inhibitor U0126.
Summary · context
Cells were cultured in Flexercell system with 10% elongation at 0.1 Hz.
In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MGF / PEG-MGF.
Report a problemFND-3108099209

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Journal Article

MeSH terms

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