Skip to content

Study · In vitro (cells) · 2019

10 results

Antioxidant and angiotensin-converting enzyme (ACE) inhibitory activity of thymosin alpha-1 (Thα1) peptide.

Kharazmi-Khorassani J, Asoodeh A, Tanzadehpanah H

Bioorganic chemistry4 Apr 2019Cited by 25 (Europe PMC)

PMID30974297 (opens in a new tab)DOI10.1016/j.bioorg.2019.04.003 (opens in a new tab)PepBankPUB-30974297
Publisher pagePubMedAsk about this study
Jump to 10 results

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study10

10 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In 1321 N1 human astrocytoma cells, Thα1 decreased the generation of reactive oxygen species.

    In plain wordsIn human nerve-type cancer cells in the lab, thymosin alpha‑1 lowered reactive oxygen species.

    Peptide
    Thymosin alpha-1
    Outcome
    DecreaseCellular ROS generation
    Study

    humann: not reported in source

    1321 N1 human neural astrocytoma cells
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Results indicated that Thα1 decreased the generation of ROS level in 1321 N1 human neural asterocytoma cells.
    1 context sentence
    Summary · context
    Moreover, the antioxidant activity of Thα1 peptide was evaluated by measuring cellular reactive oxygen species (ROS).
  2. 02LabIn vitro (cell-free)Activity measurement

    In a cell-free assay, Thα1 scavenged DPPH radicals with an IC50 of 20 µM.

    In plain wordsIn lab tests, thymosin alpha‑1 neutralized DPPH free radicals.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableDPPH radical scavenging
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    DPPH scavenging IC50
    20 µM
    Thα1

    Source sentences

    Summary
    Thα1 showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (IC50 = 20 µM) and its 2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) scavenging reached 45.33% at 80 µM (IC50 = 85 µM).
  3. 03LabIn vitro (cell-free)Activity measurement

    In a cell-free assay, Thα1 scavenged ABTS radicals, reaching 45.33% at 80 µM, with an IC50 of 85 µM.

    In plain wordsIn lab tests, thymosin alpha‑1 neutralized ABTS free radicals.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableABTS radical scavenging
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    ABTS scavenging
    45.33%
    80 µM Thα1
    ABTS scavenging IC50
    85 µM
    Thα1

    Source sentences

    Summary
    its 2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) scavenging reached 45.33% at 80 µM (IC50 = 85 µM).
  4. 04LabIn vitro (cell-free)Activity measurement

    In a cell-free assay, Thα1 scavenged hydroxyl radicals in a concentration-dependent manner, with an estimated IC50 of 82 µM.

    In plain wordsIn lab tests, thymosin alpha‑1 neutralized hydroxyl free radicals more as the concentration rose.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableHydroxyl radical scavenging
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    Hydroxyl radical scavenging IC50
    82 µM
    Thα1

    Source sentences

    Summary
    The IC50 values of hydroxyl and superoxide radical scavenging were estimated to be 82 µM and 20 µM, respectively.
    1 context sentence
    Summary · context
    In addition, hydroxyl and superoxide radical scavenging of Thα1 peptide exhibited a concentration-depended manner.
  5. 05LabIn vitro (cell-free)Activity measurement

    In a cell-free assay, Thα1 scavenged superoxide radicals with an estimated IC50 of 20 µM, and this effect (62.23%) was higher than in the other antioxidant assays.

    In plain wordsIn lab tests, thymosin alpha‑1 neutralized superoxide free radicals, and this was its strongest antioxidant effect.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableSuperoxide radical scavenging
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    Superoxide radical scavenging IC50
    20 µM
    Thα1
    Superoxide radical scavenging
    62.23%
    Thα1

    Source sentences

    Summary
    The IC50 values of hydroxyl and superoxide radical scavenging were estimated to be 82 µM and 20 µM, respectively.
    1 context sentence
    Summary · context
    The effect of Thα1 on eliminating superoxide radicals was higher (62.23%) than other antioxidant assays.
  6. 06LabIn vitro (cell-free)Activity measurement

    In a cell-free assay, Thα1 inhibited angiotensin-converting enzyme with a mixed inhibition pattern.

    In plain wordsIn lab tests, thymosin alpha‑1 blocked the enzyme ACE in a mixed way.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableACE inhibition pattern
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Based on the Lineweaver-Burk plot, Thα1 displayed a mixed inhibition pattern.
  7. 07LabIn vitro (cell-free)Activity measurement

    In a cell-free assay, Thα1 inhibited ACE with an IC50 of 0.8 µM.

    In plain wordsIn lab tests, thymosin alpha‑1 blocked the enzyme ACE at a low concentration.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableACE inhibition IC50
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    ACE inhibition IC50
    0.8 µM
    Thα1

    Source sentences

    Summary
    The IC50 and Ki values of Thα1 were 0.8 µM and 3.33 µM, respectively.
  8. 08LabIn vitro (cell-free)Activity measurement

    In a cell-free assay, the Ki of Thα1 for ACE was 3.33 µM.

    In plain wordsIn lab tests, thymosin alpha‑1 bound ACE with a measured inhibition constant of 3.33 µM.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableACE inhibition constant Ki
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    ACE inhibition Ki
    3.33 µM
    Thα1

    Source sentences

    Summary
    The IC50 and Ki values of Thα1 were 0.8 µM and 3.33 µM, respectively.
  9. 09Computer modelIn silicoMechanism

    In molecular modeling, Thα1 bound ACE domains with higher affinity for the N-domain, with a binding energy of -22.87 kcal/mol.

    In plain wordsIn computer models, thymosin alpha‑1 was predicted to bind the ACE enzyme, more tightly at its N-domain.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicablePredicted binding of Thα1 to ACE N-domain
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    Binding energy
    −22.87 kcal/mol
    Thα1 with ACE N-domain

    Source sentences

    Summary
    Molecular modeling suggested that Thα1 binds to ACE-domains with higher affinity binding to N-domain with the binding energy of -22.87 kcal/mol.
  10. 10Computer modelIn silicoMechanism

    In molecular docking, Thα1 interacted with the ACE N- and C-domains through electrostatic, hydrophobic and hydrogen forces.

    In plain wordsIn computer models, thymosin alpha‑1 was predicted to interact with both parts of the ACE enzyme.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicablePredicted interaction of Thα1 with ACE
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Molecular docking indicated that Thα1 interacted with ACE enzyme (N- and C-domains) due to electrostatic, hydrophobic, and hydrogen forces.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    10 Oct 2026, 08:55 UTC

    Study results updated

  2. Version 2

    9 Oct 2026, 03:03 UTC

    Study data updated · source update from Europe PMC

  3. Version 1

    9 Oct 2026, 03:02 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.