Oxidized alginate hydrogels with the GHK peptide enhance cord blood mesenchymal stem cell osteogenesis: A paradigm for metabolomics-based evaluation of biomaterial design.

Klontzas ME, Reakasame S, Silva R, Morais JCF, Vernardis S, MacFarlane RJ, Heliotis M, Tsiridis E, Panoskaltsis N, Boccaccini AR, Mantalaris A

Acta biomaterialia14 Feb 2019Cited by 56 (Europe PMC)

PMID30772514DOI10.1016/j.actbio.2019.02.017PepBankPUB-30772514

Publisher page PubMedAsk about this study

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: CC BY-NC-ND. Results still quote short attributed sentences.

Results from this study6

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone6

In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

01LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cells)Efficacy outcome
In umbilical cord blood stem cells, ADA-GHK hydrogels improved osteogenic differentiation compared with gelatin-containing control hydrogels.
In plain wordsIn cells, ADA-GHK hydrogels improved bone-cell differentiation compared with gelatin-containing control hydrogels.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
IncreaseOsteogenic differentiation
Study
n: not reported in source
Umbilical cord blood mesenchymal stem cells in ADA-GHK hydrogels
Comparator
Gelatin-containing control hydrogels
Outcome group
Bone and joint · Musculoskeletal
Source sentences
Summary
The ADA-GHK hydrogels significantly improved osteogenic differentiation compared to gelatin-containing control hydrogels, as demonstrated by gene expression, alkaline phosphatase activity and bone extracellular matrix deposition.
1 context sentence(design, population, supporting numbers)
Summary · context
Herein, umbilical cord blood mesenchymal stem cells (UCB MSCs) were encapsulated in novel oxidized alginate hydrogels functionalized with the glycine-histidine-lysine (GHK) peptide and differentiated towards the osteoblastic lineage.
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-3077251401
02LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cells)Efficacy outcome
In umbilical cord blood stem cells, gene expression supported improved osteogenic differentiation in ADA-GHK hydrogels versus gelatin-containing controls.
In plain wordsIn cells, gene expression supported improved bone-cell differentiation in ADA-GHK hydrogels compared with gelatin-containing controls.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
IncreaseOsteogenic gene expression
Study
n: not reported in source
Umbilical cord blood mesenchymal stem cells in ADA-GHK hydrogels
Comparator
Gelatin-containing control hydrogels
Outcome group
Bone and joint · Musculoskeletal
Source sentences
Summary
The ADA-GHK hydrogels significantly improved osteogenic differentiation compared to gelatin-containing control hydrogels, as demonstrated by gene expression, alkaline phosphatase activity and bone extracellular matrix deposition.
1 context sentence(design, population, supporting numbers)
Summary · context
Herein, umbilical cord blood mesenchymal stem cells (UCB MSCs) were encapsulated in novel oxidized alginate hydrogels functionalized with the glycine-histidine-lysine (GHK) peptide and differentiated towards the osteoblastic lineage.
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-3077251402
03LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cells)Efficacy outcome
In umbilical cord blood stem cells, alkaline phosphatase activity supported improved osteogenic differentiation in ADA-GHK hydrogels versus gelatin-containing controls.
In plain wordsIn cells, alkaline phosphatase activity supported improved bone-cell differentiation in ADA-GHK hydrogels compared with gelatin-containing controls.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
IncreaseAlkaline phosphatase activity
Study
n: not reported in source
Umbilical cord blood mesenchymal stem cells in ADA-GHK hydrogels
Comparator
Gelatin-containing control hydrogels
Outcome group
Bone and joint · Musculoskeletal
Source sentences
Summary
The ADA-GHK hydrogels significantly improved osteogenic differentiation compared to gelatin-containing control hydrogels, as demonstrated by gene expression, alkaline phosphatase activity and bone extracellular matrix deposition.
1 context sentence(design, population, supporting numbers)
Summary · context
Herein, umbilical cord blood mesenchymal stem cells (UCB MSCs) were encapsulated in novel oxidized alginate hydrogels functionalized with the glycine-histidine-lysine (GHK) peptide and differentiated towards the osteoblastic lineage.
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-3077251403
04LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cells)Efficacy outcome
In umbilical cord blood stem cells, bone matrix deposition supported improved osteogenic differentiation in ADA-GHK hydrogels versus gelatin-containing controls.
In plain wordsIn cells, bone matrix deposition supported improved differentiation in ADA-GHK hydrogels compared with gelatin-containing controls.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
IncreaseBone extracellular matrix deposition
Study
n: not reported in source
Umbilical cord blood mesenchymal stem cells in ADA-GHK hydrogels
Comparator
Gelatin-containing control hydrogels
Outcome group
Bone and joint · Musculoskeletal
Source sentences
Summary
The ADA-GHK hydrogels significantly improved osteogenic differentiation compared to gelatin-containing control hydrogels, as demonstrated by gene expression, alkaline phosphatase activity and bone extracellular matrix deposition.
1 context sentence(design, population, supporting numbers)
Summary · context
Herein, umbilical cord blood mesenchymal stem cells (UCB MSCs) were encapsulated in novel oxidized alginate hydrogels functionalized with the glycine-histidine-lysine (GHK) peptide and differentiated towards the osteoblastic lineage.
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-3077251404
05LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cells)Efficacy outcome
In cells differentiated in ADA-GHK hydrogels, metabolomics showed metabolism similar to primary osteoblasts.
In plain wordsIn cells differentiated in ADA-GHK hydrogels, metabolism was similar to primary bone cells.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
Not applicableMetabolic similarity to osteoblasts
Study
n: not reported in source
Umbilical cord blood mesenchymal stem cells in ADA-GHK hydrogels
Comparator
Primary osteoblasts
Outcome group
Bone and joint · Musculoskeletal
Source sentences
Summary
confirmed the similar metabolism between differentiated cells and primary osteoblasts
1 context sentence(design, population, supporting numbers)
Summary · context
Herein, umbilical cord blood mesenchymal stem cells (UCB MSCs) were encapsulated in novel oxidized alginate hydrogels functionalized with the glycine-histidine-lysine (GHK) peptide and differentiated towards the osteoblastic lineage.
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-3077251405
06LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cells)Mechanism
In umbilical cord blood stem cells, metabolomics elucidated the metabolic mechanism of GHK-functionalized hydrogels without specifying its details.
In plain wordsIn cells, metabolomics identified a metabolic mechanism of GHK-functionalized hydrogels, but the abstract did not give details.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
Not applicableMetabolic mechanism of GHK-functionalized hydrogels
Study
n: not reported in source
Umbilical cord blood mesenchymal stem cells in ADA-GHK hydrogels
Outcome group
Receptor activity and mechanism · Musculoskeletal
Source sentences
Summary
and elucidated the metabolic mechanism responsible for the function of GHK.
1 context sentence(design, population, supporting numbers)
Summary · context
Herein, umbilical cord blood mesenchymal stem cells (UCB MSCs) were encapsulated in novel oxidized alginate hydrogels functionalized with the glycine-histidine-lysine (GHK) peptide and differentiated towards the osteoblastic lineage.
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-3077251406

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
14 Oct 2026, 05:02 UTC
Study results updated
Version 1
14 Oct 2026, 01:46 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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