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Study · Not a test of the peptide alone · 2018

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Supplementation with IL-6 and Muscle Cell Culture Conditioned Media Enhances Myogenic Differentiation of Adipose Tissue-Derived Stem Cells through STAT3 Activation.

Seo E, Kang H, Lim OK, Jun HS

International journal of molecular sciences24 May 2018Cited by 13 (Europe PMC)

PMID29882916 (opens in a new tab)PMCIDPMC6032255 (opens in a new tab)DOI10.3390/ijms19061557 (opens in a new tab)PepBankPUB-29882916
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Summary

Mature skeletal muscle cells cannot be expanded in culture systems. Therefore, it is difficult to construct an in vitro model for muscle diseases. To establish an efficient protocol for myogenic differentiation of human adipose tissue-derived stem cells (hADSCs), we investigated whether addition of IL-6 and/or myocyte-conditioned media (CM) to conventional differentiation media can shorten the differentiation period. hADSCs were differentiated to myocytes using the conventional protocol or modified with the addition of 25 pg/mL IL-6 and/or C2C12 CM (25% v/v). The expression of MyoD and myogenine mRNA was significantly higher at 5⁻6 days after differentiation using the modified protocol than with the conventional protocol. mRNA and protein expression of myosin heavy chain, a marker of myotubes, was significantly upregulated at 28 and 42 days of differentiation using the modified protocol, and the level achieved after a 4-week differentiation period was similar to that achieved at 6 weeks using the conventional protocol. The expression of p-STAT3 was significantly increased when the modified protocol was used. Similarly, addition of colivelin, a STAT3 activator, instead of IL-6 and C2C12 CM, promoted the myogenic differentiation of ADSCs. The modified protocol improved differentiation efficiency and reduced the time required for differentiation of myocytes. It might be helpful to save cost and time when preparing myocytes for cell therapies and drug discovery.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study1

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone1

In this study, Colivelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Colivelin.

  1. 01LabNot a test of Colivelin aloneIn vitro (cells)Efficacy outcome

    In human adipose-derived stem cells, colivelin promoted myogenic differentiation when substituted for IL-6 and conditioned media.

    In plain wordsIn cells, colivelin promoted development into muscle cells when substituted for IL-6 and conditioned media.

    Peptide
    Colivelin
    Outcome
    IncreaseMyogenic differentiation
    Study

    humann: not reported in source

    adipose tissue-derived stem cells
    Outcome group
    Muscle injury and repair · Musculoskeletal

    Source sentences

    Summary
    Similarly, addition of colivelin, a STAT3 activator, instead of IL-6 and C2C12 CM, promoted the myogenic differentiation of ADSCs.

    In this study, Colivelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Colivelin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    27 Oct 2026, 18:20 UTC

    Study results updated

  2. Version 1

    27 Oct 2026, 16:32 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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