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Study · Ex vivo tissue · mouse · 2017

14 results

Treatment with AICAR inhibits blastocyst development, trophectoderm differentiation and tight junction formation and function in mice.

Calder MD, Edwards NA, Betts DH, Watson AJ

Molecular human reproduction1 Nov 2017Cited by 16 (Europe PMC)

PMID28962017 (opens in a new tab)PMCIDPMC5909861 (opens in a new tab)DOI10.1093/molehr/gax050 (opens in a new tab)PepBankPUB-28962017
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study14

14 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueEfficacy outcome

    In cultured mouse embryos, prolonged AICAR treatment from the 2-cell stage decreased blastocyst formation.

    In plain wordsIn lab tests, prolonged AICAR treatment reduced blastocyst formation in mouse embryos.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseBlastocyst formation
    Study

    mousen: not reported in source

    cultured mouse embryos
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    0-2000 μM concentration series · 48 hoursAs given in this study (mouse).

    Source sentences

    Summary
    Prolonged treatment with AICAR from the 2-cell stage onward decreases blastocyst formation, reduces total cell number, embryo diameter, leads to loss of trophectoderm cell contacts and membrane zona occludens-1 staining, and increased nuclear condensation.
    2 context sentences
    Summary · context
    First, 2-cell embryos were treated with a concentration series (0-2000 μM) of AICAR for 48 h until blastocyst formation would normally occur.
    Summary · context
    Embryos were recovered from superovulated mice.
  2. 02LabEx vivo tissueEfficacy outcome

    In cultured mouse embryos, prolonged AICAR treatment reduced total cell number.

    In plain wordsIn lab tests, prolonged AICAR treatment reduced total cell numbers in mouse embryos.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseTotal cell number
    Study

    mousen: not reported in source

    cultured mouse embryos
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated · 48 hoursAs given in this study (mouse).

    Source sentences

    Summary
    Prolonged treatment with AICAR from the 2-cell stage onward decreases blastocyst formation, reduces total cell number, embryo diameter, leads to loss of trophectoderm cell contacts and membrane zona occludens-1 staining, and increased nuclear condensation.
    2 context sentences
    Summary · context
    First, 2-cell embryos were treated with a concentration series (0-2000 μM) of AICAR for 48 h until blastocyst formation would normally occur.
    Summary · context
    Embryos were recovered from superovulated mice.
  3. 03LabEx vivo tissueEfficacy outcome

    In cultured mouse embryos, prolonged AICAR treatment reduced embryo diameter.

    In plain wordsIn lab tests, prolonged AICAR treatment reduced mouse embryo diameter.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseEmbryo diameter
    Study

    mousen: not reported in source

    cultured mouse embryos
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated · 48 hoursAs given in this study (mouse).

    Source sentences

    Summary
    Prolonged treatment with AICAR from the 2-cell stage onward decreases blastocyst formation, reduces total cell number, embryo diameter, leads to loss of trophectoderm cell contacts and membrane zona occludens-1 staining, and increased nuclear condensation.
    2 context sentences
    Summary · context
    First, 2-cell embryos were treated with a concentration series (0-2000 μM) of AICAR for 48 h until blastocyst formation would normally occur.
    Summary · context
    Embryos were recovered from superovulated mice.
  4. 04LabEx vivo tissueEfficacy outcome

    In cultured mouse embryos, prolonged AICAR treatment caused loss of trophectoderm cell contacts.

    In plain wordsIn lab tests, prolonged AICAR treatment caused loss of contacts between outer cells in mouse embryos.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseTrophectoderm cell contacts
    Study

    mousen: not reported in source

    cultured mouse embryos
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated · 48 hoursAs given in this study (mouse).

    Source sentences

    Summary
    Prolonged treatment with AICAR from the 2-cell stage onward decreases blastocyst formation, reduces total cell number, embryo diameter, leads to loss of trophectoderm cell contacts and membrane zona occludens-1 staining, and increased nuclear condensation.
    2 context sentences
    Summary · context
    First, 2-cell embryos were treated with a concentration series (0-2000 μM) of AICAR for 48 h until blastocyst formation would normally occur.
    Summary · context
    Embryos were recovered from superovulated mice.
  5. 05LabEx vivo tissueEfficacy outcome

    In cultured mouse embryos, prolonged AICAR treatment caused loss of membrane zona occludens-1 staining.

    In plain wordsIn lab tests, prolonged AICAR treatment reduced membrane zona occludens-1 staining in mouse embryos.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseMembrane zona occludens-1 staining
    Study

    mousen: not reported in source

    cultured mouse embryos
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated · 48 hoursAs given in this study (mouse).

    Source sentences

    Summary
    Prolonged treatment with AICAR from the 2-cell stage onward decreases blastocyst formation, reduces total cell number, embryo diameter, leads to loss of trophectoderm cell contacts and membrane zona occludens-1 staining, and increased nuclear condensation.
    2 context sentences
    Summary · context
    First, 2-cell embryos were treated with a concentration series (0-2000 μM) of AICAR for 48 h until blastocyst formation would normally occur.
    Summary · context
    Embryos were recovered from superovulated mice.
  6. 06LabEx vivo tissueEfficacy outcome

    In cultured mouse embryos, prolonged AICAR treatment increased nuclear condensation.

    In plain wordsIn lab tests, prolonged AICAR treatment increased nuclear condensation in mouse embryos.

    Peptide
    AICAR (acadesine)
    Outcome
    IncreaseNuclear condensation
    Study

    mousen: not reported in source

    cultured mouse embryos
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated · 48 hoursAs given in this study (mouse).

    Source sentences

    Summary
    Prolonged treatment with AICAR from the 2-cell stage onward decreases blastocyst formation, reduces total cell number, embryo diameter, leads to loss of trophectoderm cell contacts and membrane zona occludens-1 staining, and increased nuclear condensation.
    2 context sentences
    Summary · context
    First, 2-cell embryos were treated with a concentration series (0-2000 μM) of AICAR for 48 h until blastocyst formation would normally occur.
    Summary · context
    Embryos were recovered from superovulated mice.
  7. 07LabEx vivo tissueMechanism

    In cultured mouse embryos, AICAR altered mRNA levels of blastocyst formation genes.

    In plain wordsIn lab tests, AICAR changed blastocyst formation gene mRNA levels in mouse embryos, with no direction specified.

    Peptide
    AICAR (acadesine)
    Outcome
    Direction not reportedBlastocyst formation gene mRNA levels
    Study

    mousen: not reported in source

    cultured mouse embryos
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    AICAR treated embryos displayed altered mRNA and protein levels of blastocyst formation genes.
    1 context sentence
    Summary · context
    Embryos were recovered from superovulated mice.
  8. 08LabEx vivo tissueMechanism

    In cultured mouse embryos, AICAR altered protein levels of blastocyst formation genes.

    In plain wordsIn lab tests, AICAR changed blastocyst formation gene protein levels in mouse embryos, with no direction specified.

    Peptide
    AICAR (acadesine)
    Outcome
    Direction not reportedBlastocyst formation gene protein levels
    Study

    mousen: not reported in source

    cultured mouse embryos
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    AICAR treated embryos displayed altered mRNA and protein levels of blastocyst formation genes.
    1 context sentence
    Summary · context
    Embryos were recovered from superovulated mice.
  9. 09LabEx vivo tissueEfficacy outcome

    In cultured mouse blastocysts, AICAR treatment for 9 h induced collapse.

    In plain wordsIn lab tests, AICAR caused mouse blastocysts to collapse.

    Peptide
    AICAR (acadesine)
    Outcome
    IncreaseBlastocyst collapse
    Study

    mousen: not reported in source

    cultured mouse embryos
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    1000 μM · 9 hoursAs given in this study (mouse).

    Source sentences

    Summary
    Treatment of blastocysts with AICAR for 9 h induced blastocyst collapse, altered blastocyst formation gene expression, increased tight junction permeability and decreased CDX2.
    2 context sentences
    Summary · context
    In the second experiment, expanded mouse blastocysts were treated for 9 h with 1000 μM AICAR.
    Summary · context
    Embryos were recovered from superovulated mice.
  10. 10LabEx vivo tissueMechanism

    In cultured mouse blastocysts, AICAR altered blastocyst formation gene expression after 9 h.

    In plain wordsIn lab tests, AICAR changed blastocyst formation gene expression in mouse blastocysts, with no direction specified.

    Peptide
    AICAR (acadesine)
    Outcome
    Direction not reportedBlastocyst formation gene expression after 9 hours
    Study

    mousen: not reported in source

    cultured mouse embryos
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    1000 μM · 9 hoursAs given in this study (mouse).

    Source sentences

    Summary
    Treatment of blastocysts with AICAR for 9 h induced blastocyst collapse, altered blastocyst formation gene expression, increased tight junction permeability and decreased CDX2.
    2 context sentences
    Summary · context
    In the second experiment, expanded mouse blastocysts were treated for 9 h with 1000 μM AICAR.
    Summary · context
    Embryos were recovered from superovulated mice.
  11. 11LabEx vivo tissueEfficacy outcome

    In cultured mouse blastocysts, AICAR increased tight junction permeability after 9 h.

    In plain wordsIn lab tests, AICAR increased tight junction permeability in mouse blastocysts.

    Peptide
    AICAR (acadesine)
    Outcome
    IncreaseTight junction permeability
    Study

    mousen: not reported in source

    cultured mouse embryos
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    1000 μM · 9 hoursAs given in this study (mouse).

    Source sentences

    Summary
    Treatment of blastocysts with AICAR for 9 h induced blastocyst collapse, altered blastocyst formation gene expression, increased tight junction permeability and decreased CDX2.
    2 context sentences
    Summary · context
    In the second experiment, expanded mouse blastocysts were treated for 9 h with 1000 μM AICAR.
    Summary · context
    Embryos were recovered from superovulated mice.
  12. 12LabEx vivo tissueMechanism

    In cultured mouse blastocysts, AICAR decreased CDX2 after 9 h.

    In plain wordsIn lab tests, AICAR reduced CDX2 in mouse blastocysts.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseCDX2
    Study

    mousen: not reported in source

    cultured mouse embryos
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    1000 μM · 9 hoursAs given in this study (mouse).

    Source sentences

    Summary
    Treatment of blastocysts with AICAR for 9 h induced blastocyst collapse, altered blastocyst formation gene expression, increased tight junction permeability and decreased CDX2.
    2 context sentences
    Summary · context
    In the second experiment, expanded mouse blastocysts were treated for 9 h with 1000 μM AICAR.
    Summary · context
    Embryos were recovered from superovulated mice.
  13. 13LabEx vivo tissueEfficacy outcome

    In cultured mouse blastocysts, AICAR produced unaffected, reversible-effect and irreversible-effect phenotypes.

    In plain wordsIn lab tests, AICAR effects in mouse blastocysts were absent, reversible or irreversible.

    Peptide
    AICAR (acadesine)
    Outcome
    MixedTreatment response and reversibility
    Study

    mousen: not reported in source

    cultured mouse embryos
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Treated blastocysts displayed three phenotypes: those that were unaffected by treatment, those in which treatment was reversible, and those in which effects were irreversible.
    1 context sentence
    Summary · context
    Embryos were recovered from superovulated mice.
  14. 14LabEx vivo tissueMechanism

    In cultured mouse embryos, AICAR increased AMPK activity, although some outcomes may involve non-AMPK effects.

    In plain wordsIn lab tests, AICAR increased AMPK activity in mouse embryos, but some effects may involve other pathways.

    Peptide
    AICAR (acadesine)
    Outcome
    IncreaseAMPK activity
    Study

    mousen: not reported in source

    cultured mouse embryos
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    While AICAR does increase AMPK activity and this is demonstrated in our study, AICAR is not a natural regulator of AMPK activity and some outcomes may result from off target non-AMPK AICAR regulated events.
    1 context sentence
    Summary · context
    Embryos were recovered from superovulated mice.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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