GABA, Selank, and Olanzapine Affect the Expression of Genes Involved in GABAergic Neurotransmission in IMR-32 Cells.

Filatova E, Kasian A, Kolomin T, Rybalkina E, Alieva A, Andreeva L, Limborska S, Myasoedov N, Pavlova G, Slominsky P, Shadrina M

Frontiers in pharmacology28 Feb 2017Cited by 7 (Europe PMC)

PMID28293190PMCIDPMC5328971DOI10.3389/fphar.2017.00089PepBankPUB-28293190

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Summary

Clinical studies have shown that Selank had an anxiolytic effect comparable to that of classical benzodiazepine drugs, which can enhance the inhibitory effect of GABA by allosteric modulation of GABA_A receptors. These data suggest that the molecular mechanism of the effect of Selank may also be related to its ability to affect the performance of the GABAergic system. To test this hypothesis, we studied the changes in expression of 84 genes involved in the functioning of the GABAergic system and in the processes of neurotransmission in the culture of neuroblastoma IMR-32 cells using qPCR method. As test substances, in addition to Selank, we selected the major GABA_A receptor ligand, GABA, the atypical antipsychotic, olanzapine, and combinations of these compounds (Selank and GABA; Selank and olanzapine). We found no changes in the mRNA levels of the genes studied under the effect of Selank. The combined effect of GABA and Selank led to nearly complete suppression of changes in expression of genes in which mRNA levels changed under the effect of GABA. When Selank was used in conjunction with olanzapine, the expression alterations of more genes were observed compared with olanzapine alone. The data obtained indicate that Selank has no direct effect on the mRNA levels of the GABAergic system genes in neuroblastoma IMR-32 cells. At the same time, our results partially confirm the hypothesis that the peptide may affect the interaction of GABA with GABA_A receptors. Our data also suggest that Selank may enhance the effect of olanzapine on the expression of the genes studied.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study3

3 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In IMR-32 neuroblastoma cells, Selank alone did not change mRNA levels of the studied GABAergic genes.
In plain wordsIn cells, Selank alone did not change the activity of the studied GABA-system genes.
Peptide
Selank
Outcome
No significant changemRNA levels of 84 GABAergic system genes (Selank alone)
Study
n: not reported in source
IMR-32 neuroblastoma cells
Outcome group
Gene expression · Nervous system and cognition
Studied dose
Dose not stated
Source sentences
Summary
We found no changes in the mRNA levels of the genes studied under the effect of Selank.
1 context sentence(design, population, supporting numbers)
Summary · context
To test this hypothesis, we studied the changes in expression of 84 genes involved in the functioning of the GABAergic system and in the processes of neurotransmission in the culture of neuroblastoma IMR-32 cells using qPCR method.
Show in the study text Report a problemFND-2829319001
02LabIn vitro (cells)Mechanism
In IMR-32 cells, combined GABA and Selank almost completely suppressed the gene expression changes seen with GABA alone.
In plain wordsIn cells, adding Selank to GABA almost removed the gene activity changes that GABA caused alone.
Peptide
Selank
Outcome
DecreaseGene expression changes caused by GABA, with Selank added
Study
n: not reported in source
IMR-32 neuroblastoma cells
Comparator
GABA alone
Outcome group
Gene expression · Nervous system and cognition
Studied dose
Dose not stated
Source sentences
Summary
The combined effect of GABA and Selank led to nearly complete suppression of changes in expression of genes in which mRNA levels changed under the effect of GABA.
1 context sentence(design, population, supporting numbers)
Summary · context
To test this hypothesis, we studied the changes in expression of 84 genes involved in the functioning of the GABAergic system and in the processes of neurotransmission in the culture of neuroblastoma IMR-32 cells using qPCR method.
Show in the study text Report a problemFND-2829319002
03LabIn vitro (cells)Mechanism
In IMR-32 cells, Selank used with olanzapine led to expression alterations of more genes than olanzapine alone.
In plain wordsIn cells, Selank together with olanzapine changed more genes than olanzapine alone.
Peptide
Selank
Outcome
IncreaseGene expression changes with olanzapine, with Selank added
Study
n: not reported in source
IMR-32 neuroblastoma cells
Comparator
Olanzapine alone
Outcome group
Gene expression · Nervous system and cognition
Studied dose
Dose not stated
Source sentences
Summary
When Selank was used in conjunction with olanzapine, the expression alterations of more genes were observed compared with olanzapine alone.
1 context sentence(design, population, supporting numbers)
Summary · context
To test this hypothesis, we studied the changes in expression of 84 genes involved in the functioning of the GABAergic system and in the processes of neurotransmission in the culture of neuroblastoma IMR-32 cells using qPCR method.
Show in the study text Report a problemFND-2829319003

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Version 2Current
9 Oct 2026, 19:08 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

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