Skip to content

Study · In vitro (cells) · 2015

5 results

Temporal cAMP Signaling Selectivity by Natural and Synthetic MC4R Agonists.

Molden BM, Cooney KA, West K, Van Der Ploeg LH, Baldini G

Molecular endocrinology (Baltimore, Md.)29 Sept 2015Cited by 27 (Europe PMC)

PMID26418335 (opens in a new tab)PMCIDPMC4627605 (opens in a new tab)DOI10.1210/me.2015-1071 (opens in a new tab)PepBankPUB-26418335
Source linkPubMedAsk about this study
Jump to 5 results

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In Neuro2A cells, MTII induced a cAMP signal that persisted for at least 1 hour after removal of MTII, unlike alpha-MSH.

    In plain wordsIn cells, melanotan II caused a cAMP signal that lasted at least an hour after it was washed away.

    Peptide
    Melanotan II
    Outcome
    IncreasePersistence of cAMP signal after MTII removal
    Study

    n: not reported in source

    Neuro2A cells expressing MC4R
    Comparator
    alpha-MSH
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Measurements

    cAMP signal persistence
    ≥ 1 hour
    after MTII removal

    Source sentences

    Summary
    By contrast, the synthetic agonist melanotan II (MTII) induces a cAMP signal that persists for at least 1 hour after removal of MTII from the medium
    1 context sentence
    Summary · context
    We asked whether synthetic MC4R agonists differ in their ability to increase intracellular cAMP over time in Neuro2A cells expressing endogenous MC4R and exogenous, epitope-tagged hemagglutinin-MC4R-green fluorescent protein.
  2. 02LabIn vitro (cells)Mechanism

    In Neuro2A cells, the MTII-induced cAMP signal could not be antagonized by agouti related protein.

    In plain wordsIn cells, agouti related protein could not block the cAMP signal caused by melanotan II.

    Peptide
    Melanotan II
    Outcome
    No significant changeAntagonism of MTII cAMP signal by agouti related protein
    Study

    n: not reported in source

    Neuro2A cells expressing MC4R
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    and cannot be antagonized by agouti related protein.
    1 context sentence
    Summary · context
    We asked whether synthetic MC4R agonists differ in their ability to increase intracellular cAMP over time in Neuro2A cells expressing endogenous MC4R and exogenous, epitope-tagged hemagglutinin-MC4R-green fluorescent protein.
  3. 03LabIn vitro (cells)Mechanism

    In mHypoE-42 hypothalamic neurons, MTII but not alpha-MSH induced a persistent AMP kinase signal.

    In plain wordsIn cultured brain neurons, melanotan II, but not alpha-MSH, caused a lasting AMP kinase signal.

    Peptide
    Melanotan II
    Outcome
    IncreaseAMP kinase signal in hypothalamic neurons
    Study

    n: not reported in source

    mHypoE-42 immortalized hypothalamic neurons
    Comparator
    alpha-MSH
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Similarly, in mHypoE-42 immortalized hypothalamic neurons, MTII, but not α-MSH, induced persistent AMP kinase signal, which occurs downstream of increased cAMP.
    1 context sentence
    Summary · context
    We asked whether synthetic MC4R agonists differ in their ability to increase intracellular cAMP over time in Neuro2A cells expressing endogenous MC4R and exogenous, epitope-tagged hemagglutinin-MC4R-green fluorescent protein.
  4. 04LabIn vitro (cells)Mechanism

    In Neuro2A cells, the MC4R exposed to MTII appeared to continue to signal after being internalized.

    In plain wordsIn cells, the receptor exposed to melanotan II seemed to keep signaling after moving inside the cell.

    Peptide
    Melanotan II
    Outcome
    IncreaseSignaling after receptor internalization
    Study

    n: not reported in source

    Neuro2A cells expressing MC4R
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    By using a fluorescence recovery after photobleaching assay, it appears that the receptor exposed to MTII continues to signal after being internalized.
    1 context sentence
    Summary · context
    We asked whether synthetic MC4R agonists differ in their ability to increase intracellular cAMP over time in Neuro2A cells expressing endogenous MC4R and exogenous, epitope-tagged hemagglutinin-MC4R-green fluorescent protein.
  5. 05LabIn vitro (cells)Mechanism

    In Neuro2A cells, agonist-exposed MC4R desensitized to the same extent regardless of the ligand used, MTII included.

    In plain wordsIn cells, the receptor lost responsiveness equally with every agonist tested, melanotan II included.

    Peptide
    Melanotan II
    Outcome
    No significant changeMC4R desensitization after agonist exposure
    Study

    n: not reported in source

    Neuro2A cells expressing MC4R
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    However, agonist-exposed MC4R desensitized to the same extent, regardless of the ligand used and regardless of differences in receptor intracellular retention kinetics.
    1 context sentence
    Summary · context
    We asked whether synthetic MC4R agonists differ in their ability to increase intracellular cAMP over time in Neuro2A cells expressing endogenous MC4R and exogenous, epitope-tagged hemagglutinin-MC4R-green fluorescent protein.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.