Low Oxygen Tension Primes Aortic Endothelial Cells to the Reparative Effect of Tissue-Protective Cytokines.

Heikal L, Ghezzi P, Mengozzi M, Ferns G

Molecular medicine (Cambridge, Mass.)1 Sept 2015Cited by 5 (Europe PMC)

PMID26349058PMCIDPMC4749498DOI10.2119/molmed.2015.00162PepBankPUB-26349058

Summary

Erythropoietin (EPO) has both erythropoietic and tissue-protective properties. The EPO analogues carbamylated EPO (CEPO) and pyroglutamate helix B surface peptide (pHBSP) lack the erythropoietic activity of EPO but retain the tissue-protective properties that are mediated by a heterocomplex of EPO receptor (EPOR) and the β common receptor (βCR). We studied the action of EPO and its analogues in a model of wound healing where a bovine aortic endothelial cells (BAECs) monolayer was scratched and the scratch closure was assessed over 24 h under different oxygen concentrations. We related the effects of EPO and its analogues on repair to their effect on BAECs proliferation and migration (evaluated using a micro-Boyden chamber). EPO, CEPO and pHBSP enhanced scratch closure only at lower oxygen (5%), while their effect at atmospheric oxygen (21%) was not significant. The mRNA expression of EPOR was doubled in 5% compared with 21% oxygen, and this was associated with increased EPOR assessed by immunofluorescence and Western blot. By contrast, βCR mRNA levels were similar in 5% and 21% oxygen. EPO and its analogues increased both BAECs proliferation and migration, suggesting that both may be involved in the reparative process. The priming effect of low oxygen tension on the action of tissue-protective cytokines may be of relevance to vascular disease, including atherogenesis and restenosis.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study4

4 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Activity measurement
In bovine aortic endothelial cells, pHBSP, like EPO and carbamylated EPO, enhanced scratch closure at 5% oxygen.
In plain wordsIn cells, pHBSP helped a scratch in a layer of blood-vessel cells close at low oxygen.
Peptide
Cibinetide (ARA-290)
Outcome
IncreaseScratch closure at 5% oxygen
Study
cown: not reported in source
Bovine aortic endothelial cell monolayer scratch assay
Outcome group
Wound healing · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
EPO, CEPO and pHBSP enhanced scratch closure only at lower oxygen (5%)
1 context sentence(design, population, supporting numbers)
Summary · context
We studied the action of EPO and its analogues in a model of wound healing where a bovine aortic endothelial cells (BAECs) monolayer was scratched and the scratch closure was assessed over 24 h under different oxygen concentrations.
Show in the study text Report a problemFND-2634905801
02LabIn vitro (cells)Activity measurement
In bovine aortic endothelial cells, the effect of pHBSP on scratch closure at atmospheric oxygen (21%) was not significant.
In plain wordsIn cells, pHBSP did not clearly help a scratch close at normal air oxygen.
Peptide
Cibinetide (ARA-290)
Outcome
No significant changeScratch closure at 21% oxygen
Study
cown: not reported in source
Bovine aortic endothelial cell monolayer scratch assay
Outcome group
Wound healing · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
while their effect at atmospheric oxygen (21%) was not significant
1 context sentence(design, population, supporting numbers)
Summary · context
We studied the action of EPO and its analogues in a model of wound healing where a bovine aortic endothelial cells (BAECs) monolayer was scratched and the scratch closure was assessed over 24 h under different oxygen concentrations.
Show in the study text Report a problemFND-2634905802
03LabIn vitro (cells)Activity measurement
In bovine aortic endothelial cells, pHBSP, like EPO and carbamylated EPO, increased cell proliferation.
In plain wordsIn cells, pHBSP increased the growth of blood-vessel cells.
Peptide
Cibinetide (ARA-290)
Outcome
IncreaseEndothelial cell proliferation
Study
cown: not reported in source
Bovine aortic endothelial cell monolayer scratch assay
Outcome group
Wound healing · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
EPO and its analogues increased both BAECs proliferation and migration, suggesting that both may be involved in the reparative process.
2 context sentences(design, population, supporting numbers)
Summary · context
The EPO analogues carbamylated EPO (CEPO) and pyroglutamate helix B surface peptide (pHBSP)
Summary · context
We studied the action of EPO and its analogues in a model of wound healing where a bovine aortic endothelial cells (BAECs) monolayer was scratched and the scratch closure was assessed over 24 h under different oxygen concentrations.
Show in the study text Report a problemFND-2634905803
04LabIn vitro (cells)Activity measurement
In bovine aortic endothelial cells, pHBSP, like EPO and carbamylated EPO, increased cell migration.
In plain wordsIn cells, pHBSP increased the movement of blood-vessel cells.
Peptide
Cibinetide (ARA-290)
Outcome
IncreaseEndothelial cell migration
Study
cown: not reported in source
Bovine aortic endothelial cell monolayer scratch assay
Outcome group
Wound healing · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
EPO and its analogues increased both BAECs proliferation and migration, suggesting that both may be involved in the reparative process.
2 context sentences(design, population, supporting numbers)
Summary · context
The EPO analogues carbamylated EPO (CEPO) and pyroglutamate helix B surface peptide (pHBSP)
Summary · context
We studied the action of EPO and its analogues in a model of wound healing where a bovine aortic endothelial cells (BAECs) monolayer was scratched and the scratch closure was assessed over 24 h under different oxygen concentrations.
Show in the study text Report a problemFND-2634905804

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

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Version 2Current
10 Oct 2026, 10:21 UTC
Study results updated
Version 1
9 Oct 2026, 03:13 UTC
First added · source update from Europe PMC

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