MRP4 Modulation of the Guanylate Cyclase-C/cGMP Pathway: Effects on Linaclotide-Induced Electrolyte Secretion and cGMP Efflux.

Tchernychev B, Ge P, Kessler MM, Solinga RM, Wachtel D, Tobin JV, Thomas SR, Lunte CE, Fretzen A, Hannig G, Bryant AP, Kurtz CB, Currie MG, Silos-Santiago I

The Journal of pharmacology and experimental therapeutics27 Jul 2015Cited by 19 (Europe PMC)

PMID26216942DOI10.1124/jpet.115.224329PepBankPUB-26216942

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

3 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueMechanism
In rat colonic mucosa, linaclotide increased transepithelial ion current concentration-dependently, with EC50 of 9.2 nM.
In plain wordsIn lab tests with rat colon tissue, linaclotide increased ion current as concentration increased.
Peptide
Linaclotide
Outcome
IncreaseTransepithelial ion current
Study
ratn: not reported in source
colonic mucosal layer in Ussing chamber
Outcome group
Receptor activity and mechanism · Gastrointestinal
Studied dose
Dose not stated
Measurements
EC50
9.2 nM
transepithelial ion current
Source sentences
Summary
Linaclotide activation of the guanylate cyclase-C (GC-C)/cGMP pathway induced a concentration-dependent increase in transepithelial ion current [short-circuit current (Isc)] across rat colonic mucosa (EC50: 9.2 nM).
1 context sentence(design, population, supporting numbers)
Summary · context
In vitro studies were performed using a rat colonic mucosal layer mounted in an Ussing chamber.
Report a problemFND-2621694201
02LabEx vivo tissueMechanism
In rat colonic epithelium, linaclotide activation of GC-C induced apical cGMP secretion.
In plain wordsIn lab tests with rat colon tissue, linaclotide induced cGMP secretion from the apical membrane.
Peptide
Linaclotide
Outcome
IncreaseApical cGMP secretion
Study
ratn: not reported in source
colonic mucosal layer in Ussing chamber
Outcome group
Receptor activity and mechanism · Gastrointestinal
Studied dose
Dose not stated
Source sentences
Summary
Furthermore, linaclotide activation of GC-C induced cGMP secretion from the apical and basolateral membranes of colonic epithelium.
1 context sentence(design, population, supporting numbers)
Summary · context
In vitro studies were performed using a rat colonic mucosal layer mounted in an Ussing chamber.
Report a problemFND-2621694202
03LabEx vivo tissueMechanism
In rat colonic epithelium, linaclotide activation of GC-C induced basolateral cGMP secretion.
In plain wordsIn lab tests with rat colon tissue, linaclotide induced cGMP secretion from the basolateral membrane.
Peptide
Linaclotide
Outcome
IncreaseBasolateral cGMP secretion
Study
ratn: not reported in source
colonic mucosal layer in Ussing chamber
Outcome group
Receptor activity and mechanism · Gastrointestinal
Studied dose
Dose not stated
Source sentences
Summary
Furthermore, linaclotide activation of GC-C induced cGMP secretion from the apical and basolateral membranes of colonic epithelium.
1 context sentence(design, population, supporting numbers)
Summary · context
In vitro studies were performed using a rat colonic mucosal layer mounted in an Ussing chamber.
Report a problemFND-2621694203

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
28 Oct 2026, 02:03 UTC
Study results updated
Version 1
27 Oct 2026, 16:34 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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