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Study · Animal study (in vivo) · rat · 2015

5 results

Systemic application of teriparatide for steroid induced osteonecrosis in a rat model.

Dong Y, Li Y, Huang C, Gao K, Weng X

BMC musculoskeletal disorders11 Jul 2015Cited by 33 (Europe PMC)

PMID26163144 (opens in a new tab)PMCIDPMC4499203 (opens in a new tab)DOI10.1186/s12891-015-0589-z (opens in a new tab)PepBankPUB-26163144
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Summary

BackgroundSteroid associated osteonecrosis is difficult to treat. Teriparatide, as the only one bone anabolic drug, has achieved very promising effect in osteoporosis and other bone skeletal diseases. We carried out this animal study to evaluate the effect of subcutaneous injection of teriparatide for the steroid induced femoral head necrosis in a rat model.Methods24 Sprague-Dawley male adult rats were included in the study. All the rats were randomized into 4 groups: 18 rats from LPS/MPS group, LPS/MPS + PTH group and LPS/MPS + NS group were given lipopolysaccharide (20 μg/kg) and methylprednisolone (40 mg/kg) to establish the steroid induced osteonecrosis model. 6 rats from NS group only received normal saline. 4 weeks later, All the rats in LPS/MPS group and NS group were sacrificed and the femoral heads were harvested. After that, the 6 rats in the LPS/MPS + PTH group received subcutaneous injection of 20 μg/kg teriparatide and LPS/MPS + NS group only received equal amount of normal saline. After 4 weeks, the serum bone marker was tested and the femoral head were harvested. Micro-CT and histological examination were performed to compare the incidence of osteonecrosis and trabeculae parameters for the femoral head.ResultsAt 4 weeks, rats in LPS/MPS group showed significant osteonecrosis by histological examination (83.3 %) which suggested successful steroid induced osteronecrosis animal models were established. After the treatment of 4 weeks, the LPS/MPS + PTH group showed significant lower incidence rate of osteonecrosis compared with the LPS/MPS + NS group (16.7 % vs.75 %, P < 0.05). The micro CT examination showed higher bone volume/total volume, trabecula thickness and bone mineral density in the LPS/MPS + PTH group compared with the LPS/MPS + NS group. The serum osteocalcin was a little higher in the LPS/MPS + PTH group (4.54 ± 1.61vs.3.58 ± 1.81, P = 0.358), but it didn't reach a statistical significance.ConclusionsSystemic application of teriparatide for steroid induced osteonecrosis in rats showed a beneficial effect. This may be one promising therapy for early stage osteonecrosis.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study5

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In rats with steroid-induced osteonecrosis, teriparatide reduced osteonecrosis incidence versus saline.

    In plain wordsIn rats, teriparatide reduced osteonecrosis incidence compared with saline.

    Peptide
    Teriparatide (PTH 1-34)
    Outcome
    DecreaseOsteonecrosis incidence
    Study

    ratn: not reported in source

    steroid-induced femoral head osteonecrosis
    Comparator
    normal saline
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    20 μg/kg · subcutaneous · 4 weeksAs given in this study (rat).

    Measurements

    Osteonecrosis incidence
    16.7%
    teriparatide
    Osteonecrosis incidence
    75%
    saline

    Source sentences

    Summary
    After the treatment of 4 weeks, the LPS/MPS + PTH group showed significant lower incidence rate of osteonecrosis compared with the LPS/MPS + NS group (16.7 % vs.75 %, P < 0.05).
    1 context sentence
    Summary · context
    After that, the 6 rats in the LPS/MPS + PTH group received subcutaneous injection of 20 μg/kg teriparatide and LPS/MPS + NS group only received equal amount of normal saline. After 4 weeks, the serum bone marker was tested and the femoral head were harvested.
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In rats, teriparatide increased bone volume/total volume compared with saline.

    In plain wordsIn rats, teriparatide increased bone volume/total volume compared with saline.

    Peptide
    Teriparatide (PTH 1-34)
    Outcome
    IncreaseBone volume/total volume
    Study

    ratn: not reported in source

    steroid-induced femoral head osteonecrosis
    Comparator
    normal saline
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    20 μg/kg · subcutaneous · 4 weeksAs given in this study (rat).

    Source sentences

    Summary
    The micro CT examination showed higher bone volume/total volume, trabecula thickness and bone mineral density in the LPS/MPS + PTH group compared with the LPS/MPS + NS group.
    1 context sentence
    Summary · context
    After that, the 6 rats in the LPS/MPS + PTH group received subcutaneous injection of 20 μg/kg teriparatide and LPS/MPS + NS group only received equal amount of normal saline. After 4 weeks, the serum bone marker was tested and the femoral head were harvested.
  3. 03AnimalAnimal study (in vivo)Efficacy outcome

    In rats, teriparatide increased trabecular thickness compared with saline.

    In plain wordsIn rats, teriparatide increased trabecular thickness compared with saline.

    Peptide
    Teriparatide (PTH 1-34)
    Outcome
    IncreaseTrabecular thickness
    Study

    ratn: not reported in source

    steroid-induced femoral head osteonecrosis
    Comparator
    normal saline
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    20 μg/kg · subcutaneous · 4 weeksAs given in this study (rat).

    Source sentences

    Summary
    The micro CT examination showed higher bone volume/total volume, trabecula thickness and bone mineral density in the LPS/MPS + PTH group compared with the LPS/MPS + NS group.
    1 context sentence
    Summary · context
    After that, the 6 rats in the LPS/MPS + PTH group received subcutaneous injection of 20 μg/kg teriparatide and LPS/MPS + NS group only received equal amount of normal saline. After 4 weeks, the serum bone marker was tested and the femoral head were harvested.
  4. 04AnimalAnimal study (in vivo)Efficacy outcome

    In rats, teriparatide increased bone mineral density compared with saline.

    In plain wordsIn rats, teriparatide increased bone mineral density compared with saline.

    Peptide
    Teriparatide (PTH 1-34)
    Outcome
    IncreaseBone mineral density
    Study

    ratn: not reported in source

    steroid-induced femoral head osteonecrosis
    Comparator
    normal saline
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    20 μg/kg · subcutaneous · 4 weeksAs given in this study (rat).

    Source sentences

    Summary
    The micro CT examination showed higher bone volume/total volume, trabecula thickness and bone mineral density in the LPS/MPS + PTH group compared with the LPS/MPS + NS group.
    1 context sentence
    Summary · context
    After that, the 6 rats in the LPS/MPS + PTH group received subcutaneous injection of 20 μg/kg teriparatide and LPS/MPS + NS group only received equal amount of normal saline. After 4 weeks, the serum bone marker was tested and the femoral head were harvested.
  5. 05AnimalAnimal study (in vivo)Efficacy outcome

    In rats, serum osteocalcin did not differ significantly between teriparatide and saline.

    In plain wordsIn rats, teriparatide did not significantly change serum osteocalcin compared with saline.

    Peptide
    Teriparatide (PTH 1-34)
    Outcome
    No significant changeSerum osteocalcin
    Study

    ratn: not reported in source

    steroid-induced femoral head osteonecrosis
    Comparator
    normal saline
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    20 μg/kg · subcutaneous · 4 weeksAs given in this study (rat).

    Source sentences

    Summary
    The serum osteocalcin was a little higher in the LPS/MPS + PTH group (4.54 ± 1.61vs.3.58 ± 1.81, P = 0.358), but it didn't reach a statistical significance.
    1 context sentence
    Summary · context
    After that, the 6 rats in the LPS/MPS + PTH group received subcutaneous injection of 20 μg/kg teriparatide and LPS/MPS + NS group only received equal amount of normal saline. After 4 weeks, the serum bone marker was tested and the femoral head were harvested.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    22 Oct 2026, 02:26 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 18:59 UTC

    First added · source update from Europe PMC

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