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Study · Animal study (in vivo) · mouse · 2015

4 results

[Effect of Gly-His-Lys peptide and its analogs on pain sensitivity in mice].

Bobyntsev II, Chernysheva OI, Dolgintsev ME, Smakhtin MIu, Belykh AE

Eksperimental'naia i klinicheskaia farmakologiia1 Jan 2015Cited by 1 (Europe PMC)

PMID25826868 (opens in a new tab)PepBankPUB-25826868
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

1 result from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In male BALB/c mice, Gly-His-Lys increased paw-licking latency in the hot-plate test, indicating analgesia.

    In plain wordsIn mice, Gly-His-Lys increased the time before paw licking in a hot-plate test, indicating reduced pain.

    Peptide
    GHK (glycyl-L-histidyl-L-lysine)
    Outcome
    IncreasePaw-licking latency
    Study

    micen: not reported in source

    Male BALB/c mice in the hot-plate test
    Outcome group
    Pain · Nervous system and cognition
    Studied dose
    0.5, 1.5, 5, 15, and 50 mg/kg · intraperitonealAs given in this study (mice).

    Source sentences

    Summary
    The intraperitoneal administration of Gly-His-Lys tripeptide to male BALB/c mice 12 min before the beginning of the study at doses 0.5, 1.5, 5, 15, and 50 mg/kg produced analgesic effect in the hot-plate test, which was manifested by an increase in the duration of the latent period of the paw-licking reaction.

Studies that did not test the peptide alone3

In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

  1. 02AnimalNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneAnimal study (in vivo)Efficacy outcome

    In mice, replacement of L-lysine with D-lysine weakened the analgesic effect of the Gly-His-Lys analog at the same doses.

    In plain wordsIn mice, the D-lysine analog showed weaker pain relief than unmodified Gly-His-Lys at the same doses.

    Peptide
    GHK (glycyl-L-histidyl-L-lysine)
    Outcome
    DecreaseD-lysine analog analgesia
    Study

    micen: not reported in source

    Male BALB/c mice in the hot-plate test
    Comparator
    Unmodified Gly-His-Lys
    Outcome group
    Pain · Nervous system and cognition

    Source sentences

    Summary
    The replacement of L-lysine by D-lysine in the tripeptide molecule was accompanied by significant weakening of the analgesic effect after administration in the same doses.

    In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

  2. 03AnimalNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneAnimal study (in vivo)Efficacy outcome

    In mice, attachment of D-alanine to either end of Gly-His-Lys leveled its analgesic effect.

    In plain wordsIn mice, D-alanine attachment removed the pain-relieving effect reported for unmodified Gly-His-Lys.

    Peptide
    GHK (glycyl-L-histidyl-L-lysine)
    Outcome
    DecreaseD-alanine analog analgesia
    Study

    micen: not reported in source

    Male BALB/c mice in the hot-plate test
    Comparator
    Unmodified Gly-His-Lys
    Outcome group
    Pain · Nervous system and cognition

    Source sentences

    Summary
    The attachment of D-alanine to N- or C-end of Gly-His-Lys peptide led to leveling of the analgesic effect.

    In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

  3. 04AnimalNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneAnimal study (in vivo)Efficacy outcome

    In mice, modified Gly-His-Lys analogs increased paw-licking latency in almost all groups, which the abstract interpreted as algic effects.

    In plain wordsIn mice, modified Gly-His-Lys analogs increased paw-licking latency, which the abstract interpreted as pain-promoting effects.

    Peptide
    GHK (glycyl-L-histidyl-L-lysine)
    Outcome
    IncreaseModified peptide paw-licking latency
    Study

    micen: not reported in source

    Male BALB/c mice in the hot-plate test
    Outcome group
    Pain · Nervous system and cognition

    Source sentences

    Summary
    On the contrary, after the administration of these analogs, the duration of the latent period of the paw-licking reaction was increased in almost all experimental groups of animals and reached in some cases significant differences that were indicative of the manifestation of algic effects of the modified peptides.

    In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    14 Oct 2026, 05:02 UTC

    Study results updated

  2. Version 1

    14 Oct 2026, 01:46 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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