Mechano-growth factor accelerates the proliferation and osteogenic differentiation of rabbit mesenchymal stem cells through the PI3K/AKT pathway.

Tong Y, Feng W, Wu Y, Lv H, Jia Y, Jiang D

BMC biochemistry15 Jan 2015Cited by 24 (Europe PMC)

PMID25588515PMCIDPMC4299473DOI10.1186/s12858-015-0031-zPepBankPUB-25588515

Summary

BackgroundMesenchymal stem cells (MSCs) can differentiate into chondroblasts, adipocytes, or osteoblasts under appropriate stimulation. Mechano-growth factor (MGF) reportedly displays a neuroprotective effect in cerebral regions that were exposed to ischemia and is expressed in stromal cells of the eutopic endometrium and in glandular cells of the ectopic endometrium.ResultsThis study sought to understand the potential involvement of phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT) in MGF-induced growth of rabbit MSCs (rMSCs). We applied various concentrations of MGF to cultured rMSCs and observed the growth rate of the cells, the changes in the phosphorylation state of AKT and mammalian target of rapamycin (mTOR), and the expression levels of alkaline phosphatase and osteocalcin. We found that the growth and osteogenic differentiation of MGF-induced rMSCs were promoted primarily by phosphorylated AKT, and that this phosphorylation, as well mTOR phosphorylation, was mediated by the MGF receptor.ConclusionOur study suggests that MGF promotes the growth and osteogenic differentiation of rMSCs primarily through the PI3K/AKT pathway.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study3

3 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In rabbit mesenchymal stem cells, MGF promoted cell growth, mainly through phosphorylated AKT.
In plain wordsIn rabbit stem cells in the lab, MGF promoted cell growth, mainly through AKT.
Peptide
MGF / PEG-MGF
Outcome
IncreaseGrowth of rMSCs
Study
rabbitn: not reported in source
Rabbit mesenchymal stem cells (rMSCs)
Outcome group
Bone and joint · Musculoskeletal
Studied dose
Dose not stated
Source sentences
Summary
We found that the growth and osteogenic differentiation of MGF-induced rMSCs were promoted primarily by phosphorylated AKT, and that this phosphorylation, as well mTOR phosphorylation, was mediated by the MGF receptor.
1 context sentence(design, population, supporting numbers)
Summary · context
We applied various concentrations of MGF to cultured rMSCs and observed the growth rate of the cells, the changes in the phosphorylation state of AKT and mammalian target of rapamycin (mTOR), and the expression levels of alkaline phosphatase and osteocalcin.
Show in the study text Report a problemFND-2558851501
02LabIn vitro (cells)Mechanism
In rabbit mesenchymal stem cells, MGF promoted osteogenic differentiation, mainly through phosphorylated AKT.
In plain wordsIn rabbit stem cells in the lab, MGF promoted bone-cell development, mainly through AKT.
Peptide
MGF / PEG-MGF
Outcome
IncreaseOsteogenic differentiation of rMSCs
Study
rabbitn: not reported in source
Rabbit mesenchymal stem cells (rMSCs)
Outcome group
Bone and joint · Musculoskeletal
Studied dose
Dose not stated
Source sentences
Summary
We found that the growth and osteogenic differentiation of MGF-induced rMSCs were promoted primarily by phosphorylated AKT, and that this phosphorylation, as well mTOR phosphorylation, was mediated by the MGF receptor.
1 context sentence(design, population, supporting numbers)
Summary · context
We applied various concentrations of MGF to cultured rMSCs and observed the growth rate of the cells, the changes in the phosphorylation state of AKT and mammalian target of rapamycin (mTOR), and the expression levels of alkaline phosphatase and osteocalcin.
Show in the study text Report a problemFND-2558851502
03LabIn vitro (cells)Mechanism
In rabbit mesenchymal stem cells, AKT phosphorylation and mTOR phosphorylation were mediated by the MGF receptor.
In plain wordsIn rabbit stem cells in the lab, the MGF receptor mediated the AKT and mTOR signals.
Peptide
MGF / PEG-MGF
Outcome
Not applicableAKT and mTOR phosphorylation mediated by MGF receptor
Study
rabbitn: not reported in source
Rabbit mesenchymal stem cells (rMSCs)
Outcome group
Bone and joint · Musculoskeletal
Studied dose
Dose not stated
Source sentences
Summary
and that this phosphorylation, as well mTOR phosphorylation, was mediated by the MGF receptor.
1 context sentence(design, population, supporting numbers)
Summary · context
We applied various concentrations of MGF to cultured rMSCs and observed the growth rate of the cells, the changes in the phosphorylation state of AKT and mammalian target of rapamycin (mTOR), and the expression levels of alkaline phosphatase and osteocalcin.
Show in the study text Report a problemFND-2558851503

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

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Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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