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Study · Not a test of the peptide alone · 2015

7 results

Stability-indicating HPLC assay for lysine-proline-valine (KPV) in aqueous solutions and skin homogenates.

Pawar KR, Mulabagal V, Smith F, Kolli CS, Rangari VK, Babu RJ

Biomedical chromatography : BMC9 Oct 2014Cited by 3 (Europe PMC)

PMID25298219 (opens in a new tab)DOI10.1002/bmc.3347 (opens in a new tab)PepBankPUB-25298219
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone7

In this study, KPV was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking KPV.

  1. 01LabNot a test of KPV aloneIn vitro (cell-free)Other

    The HPLC calibration curve for KPV was linear, with a correlation coefficient of 0.9999.

    In plain wordsIn lab tests, the measurement method for KPV was linear over its tested range.

    Peptide
    KPV
    Outcome
    Not applicableLinearity of the HPLC calibration curve
    Study

    n: not reported in source

    Outcome group
    Analytical methods and detection · Pharmacology

    Measurements

    correlation coefficient r
    0.9999 unitless
    calibration curve

    Source sentences

    Summary
    The calibration curve was linear with a correlation coefficient (r) of 0.9999.
    1 context sentence
    Summary · context
    A simple, sensitive and stability-indicating high-performance liquid chromatographic (HPLC) assay method was developed and validated for a bioactive peptide, lysine-proline-valine (KPV) in aqueous solutions and skin homogenates.

    In this study, KPV was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking KPV.

  2. 02LabNot a test of KPV aloneIn vitro (cell-free)Other

    Relative standard deviation values of the accuracy and precision experiments were below 2.

    In plain wordsIn lab tests, the measurement method for KPV gave accurate and repeatable results.

    Peptide
    KPV
    Outcome
    Not applicableAccuracy and precision of the HPLC assay
    Study

    n: not reported in source

    Outcome group
    Analytical methods and detection · Pharmacology

    Measurements

    relative standard deviation
    < 2 unitless
    accuracy and precision experiments

    Source sentences

    Summary
    Relative standard deviation values of accuracy and precision experiments were <2.
    1 context sentence
    Summary · context
    A simple, sensitive and stability-indicating high-performance liquid chromatographic (HPLC) assay method was developed and validated for a bioactive peptide, lysine-proline-valine (KPV) in aqueous solutions and skin homogenates.

    In this study, KPV was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking KPV.

  3. 03LabNot a test of KPV aloneIn vitro (cell-free)Other

    The limit of detection of the HPLC assay for KPV was 0.01 µg/mL.

    In plain wordsIn lab tests, the method could detect KPV down to 0.01 µg/mL.

    Peptide
    KPV
    Outcome
    Not applicableLimit of detection of KPV
    Study

    n: not reported in source

    Outcome group
    Analytical methods and detection · Pharmacology

    Measurements

    LOD
    0.01 µg/mL
    KPV

    Source sentences

    Summary
    The LOD and LOQ of KPV were 0.01 and 0.25 µg/mL, respectively.
    1 context sentence
    Summary · context
    A simple, sensitive and stability-indicating high-performance liquid chromatographic (HPLC) assay method was developed and validated for a bioactive peptide, lysine-proline-valine (KPV) in aqueous solutions and skin homogenates.

    In this study, KPV was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking KPV.

  4. 04LabNot a test of KPV aloneIn vitro (cell-free)Other

    The limit of quantitation of the HPLC assay for KPV was 0.25 µg/mL.

    In plain wordsIn lab tests, the method could measure KPV amounts down to 0.25 µg/mL.

    Peptide
    KPV
    Outcome
    Not applicableLimit of quantitation of KPV
    Study

    n: not reported in source

    Outcome group
    Analytical methods and detection · Pharmacology

    Measurements

    LOQ
    0.25 µg/mL
    KPV

    Source sentences

    Summary
    The LOD and LOQ of KPV were 0.01 and 0.25 µg/mL, respectively.
    1 context sentence
    Summary · context
    A simple, sensitive and stability-indicating high-performance liquid chromatographic (HPLC) assay method was developed and validated for a bioactive peptide, lysine-proline-valine (KPV) in aqueous solutions and skin homogenates.

    In this study, KPV was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking KPV.

  5. 05LabNot a test of KPV aloneIn vitro (cell-free)Other

    Under acid, alkali and hydrogen peroxide stress, KPV yielded lys-pro-diketopiperazine as the major degradation product.

    In plain wordsIn lab tests, KPV broke down mainly into lys-pro-diketopiperazine under harsh conditions.

    Peptide
    KPV
    Outcome
    Not applicableKPV degradation product under stress conditions
    Study

    n: not reported in source

    Outcome group
    Analytical methods and detection · Pharmacology

    Source sentences

    Summary
    Under stress conditions (acid, alkali and hydrogen peroxide) KPV yielded lys-pro-diketopiperazine as major degradation product, which was identified by flow injection MS analysis.
    1 context sentence
    Summary · context
    A simple, sensitive and stability-indicating high-performance liquid chromatographic (HPLC) assay method was developed and validated for a bioactive peptide, lysine-proline-valine (KPV) in aqueous solutions and skin homogenates.

    In this study, KPV was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking KPV.

  6. 06LabNot a test of KPV aloneIn vitro (cell-free)Other

    The HPLC method separated KPV from its degradation products generated under stress conditions.

    In plain wordsIn lab tests, the method told KPV apart from its breakdown products.

    Peptide
    KPV
    Outcome
    Not applicableSeparation of KPV from degradation products
    Study

    n: not reported in source

    Outcome group
    Analytical methods and detection · Pharmacology

    Source sentences

    Summary
    The developed HPLC method was found to be efficient in separating the active peptide from its degradation products generated under various stress conditions.
    1 context sentence
    Summary · context
    A simple, sensitive and stability-indicating high-performance liquid chromatographic (HPLC) assay method was developed and validated for a bioactive peptide, lysine-proline-valine (KPV) in aqueous solutions and skin homogenates.

    In this study, KPV was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking KPV.

  7. 07LabNot a test of KPV aloneIn vitro (cell-free)Other

    The validated method separated KPV from peaks arising from endogenous components of skin homogenate.

    In plain wordsIn lab tests, the method told KPV apart from natural skin components.

    Peptide
    KPV
    Outcome
    Not applicableSeparation of KPV from skin homogenate components
    Study

    n: not reported in source

    Outcome group
    Analytical methods and detection · Pharmacology

    Source sentences

    Summary
    Also, the validated method was able to separate KPV from other peaks arising from endogenous components of the skin homogenate.
    1 context sentence
    Summary · context
    A simple, sensitive and stability-indicating high-performance liquid chromatographic (HPLC) assay method was developed and validated for a bioactive peptide, lysine-proline-valine (KPV) in aqueous solutions and skin homogenates.

    In this study, KPV was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking KPV.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    9 Oct 2026, 18:17 UTC

    Study results updated

  2. Version 2

    9 Oct 2026, 03:03 UTC

    Study data updated · source update from Europe PMC

  3. Version 1

    9 Oct 2026, 02:56 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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