Neuroprotective effect of novel cognitive enhancer noopept on AD-related cellular model involves the attenuation of apoptosis and tau hyperphosphorylation.

Ostrovskaya RU, Vakhitova YV, Kuzmina USh, Salimgareeva MKh, Zainullina LF, Gudasheva TA, Vakhitov VA, Seredenin SB

Journal of biomedical science6 Aug 2014Cited by 17 (Europe PMC)

PMID25096780PMCIDPMC4422191DOI10.1186/s12929-014-0074-2PepBankPUB-25096780

Summary

BackgroundNoopept (N-phenyl-acetyl-L-prolylglycine ethyl ester) was constructed as a dipeptide analog of the standard cognition enhancer, piracetam. Our previous experiments have demonstrated the cognition restoring effect of noopept in several animal models of Alzheimer disease (AD). Noopept was also shown to prevent ionic disbalance, excitotoxicity, free radicals and pro-inflammatory cytokines accumulation, and neurotrophine deficit typical for different kinds of brain damages, including AD. In this study, we investigated the neuroprotective action of noopept on cellular model of AD, Aβ 25-35-induced toxicity in PC12 cells and revealed the underlying mechanisms.ResultsThe neuroprotective effect of noopept (added to the medium at 10 μM concentration, 72 hours before Аβ 25-35) was studied on Аβ 25-35-induced injury (5 μM for 24 h) in PC12 cells. The ability of drug to protect the impairments of cell viability, calcium homeostasis, ROS level, mitochondrial function, tau phosphorylation and neurite outgrowth caused by Аβ 25-35 were evaluated. Following the exposure of PC12 cells to Аβ 25-35 an increase of the level of ROS, intracellular calcium, and tau phosphorylation at Ser396 were observed; these changes were accompanied by a decrease in cell viability and an increase of apoptosis. Noopept treatment before the amyloid-beta exposure improved PC12 cells viability, reduced the number of early and late apoptotic cells, the levels of intracellular reactive oxygen species and calcium and enhanced the mitochondrial membrane potential. In addition, pretreatment of PC12 cell with noopept significantly attenuated tau hyperphosphorylation at Ser396 and ameliorated the alterations of neurite outgrowth evoked by Аβ25-35.ConclusionsTaken together, these data provide evidence that novel cognitive enhancer noopept protects PC12 cell against deleterious actions of Aβ through inhibiting the oxidative damage and calcium overload as well as suppressing the mitochondrial apoptotic pathway. Moreover, neuroprotective properties of noopept likely include its ability to decrease tau phosphorylation and to restore the altered morphology of PC12 cells. Therefore, this nootropic dipeptide is able to positively affect not only common pathogenic pathways but also disease-specific mechanisms underlying Aβ-related pathology.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study8

8 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Efficacy outcome
In amyloid-beta-exposed PC12 cells, Noopept pretreatment improved viability.
In plain wordsIn amyloid-beta-exposed cells, Noopept pretreatment improved cell viability compared with amyloid-beta exposure without Noopept.
Peptide
Noopept (omberacetam)
Outcome
IncreaseCell viability
Study
n: not reported in source
PC12 cells exposed to amyloid-beta 25-35
Comparator
Amyloid-beta exposure without Noopept
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
10 μM · 72 hours before Аβ 25-35As given in this study.
Source sentences
Summary
Noopept treatment before the amyloid-beta exposure improved PC12 cells viability, reduced the number of early and late apoptotic cells, the levels of intracellular reactive oxygen species and calcium and enhanced the mitochondrial membrane potential.
1 context sentence(design, population, supporting numbers)
Summary · context
The neuroprotective effect of noopept (added to the medium at 10 μM concentration, 72 hours before Аβ 25-35) was studied on Аβ 25-35-induced injury (5 μM for 24 h) in PC12 cells.
Show in the study text Report a problemFND-2509678001
02LabIn vitro (cells)Efficacy outcome
In amyloid-beta-exposed PC12 cells, Noopept pretreatment reduced early apoptotic cells.
In plain wordsIn amyloid-beta-exposed cells, Noopept pretreatment reduced early apoptotic cells compared with amyloid-beta exposure without Noopept.
Peptide
Noopept (omberacetam)
Outcome
DecreaseEarly apoptosis
Study
n: not reported in source
PC12 cells exposed to amyloid-beta 25-35
Comparator
Amyloid-beta exposure without Noopept
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
10 μM · 72 hours before Аβ 25-35As given in this study.
Source sentences
Summary
Noopept treatment before the amyloid-beta exposure improved PC12 cells viability, reduced the number of early and late apoptotic cells, the levels of intracellular reactive oxygen species and calcium and enhanced the mitochondrial membrane potential.
1 context sentence(design, population, supporting numbers)
Summary · context
The neuroprotective effect of noopept (added to the medium at 10 μM concentration, 72 hours before Аβ 25-35) was studied on Аβ 25-35-induced injury (5 μM for 24 h) in PC12 cells.
Show in the study text Report a problemFND-2509678002
03LabIn vitro (cells)Efficacy outcome
In amyloid-beta-exposed PC12 cells, Noopept pretreatment reduced late apoptotic cells.
In plain wordsIn amyloid-beta-exposed cells, Noopept pretreatment reduced late apoptotic cells compared with amyloid-beta exposure without Noopept.
Peptide
Noopept (omberacetam)
Outcome
DecreaseLate apoptosis
Study
n: not reported in source
PC12 cells exposed to amyloid-beta 25-35
Comparator
Amyloid-beta exposure without Noopept
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
10 μM · 72 hours before Аβ 25-35As given in this study.
Source sentences
Summary
Noopept treatment before the amyloid-beta exposure improved PC12 cells viability, reduced the number of early and late apoptotic cells, the levels of intracellular reactive oxygen species and calcium and enhanced the mitochondrial membrane potential.
1 context sentence(design, population, supporting numbers)
Summary · context
The neuroprotective effect of noopept (added to the medium at 10 μM concentration, 72 hours before Аβ 25-35) was studied on Аβ 25-35-induced injury (5 μM for 24 h) in PC12 cells.
Show in the study text Report a problemFND-2509678003
04LabIn vitro (cells)Efficacy outcome
In amyloid-beta-exposed PC12 cells, Noopept pretreatment reduced intracellular reactive oxygen species.
In plain wordsIn amyloid-beta-exposed cells, Noopept pretreatment reduced reactive oxygen species compared with amyloid-beta exposure without Noopept.
Peptide
Noopept (omberacetam)
Outcome
DecreaseIntracellular reactive oxygen species
Study
n: not reported in source
PC12 cells exposed to amyloid-beta 25-35
Comparator
Amyloid-beta exposure without Noopept
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
10 μM · 72 hours before Аβ 25-35As given in this study.
Source sentences
Summary
Noopept treatment before the amyloid-beta exposure improved PC12 cells viability, reduced the number of early and late apoptotic cells, the levels of intracellular reactive oxygen species and calcium and enhanced the mitochondrial membrane potential.
1 context sentence(design, population, supporting numbers)
Summary · context
The neuroprotective effect of noopept (added to the medium at 10 μM concentration, 72 hours before Аβ 25-35) was studied on Аβ 25-35-induced injury (5 μM for 24 h) in PC12 cells.
Show in the study text Report a problemFND-2509678004
05LabIn vitro (cells)Efficacy outcome
In amyloid-beta-exposed PC12 cells, Noopept pretreatment reduced intracellular calcium.
In plain wordsIn amyloid-beta-exposed cells, Noopept pretreatment reduced intracellular calcium compared with amyloid-beta exposure without Noopept.
Peptide
Noopept (omberacetam)
Outcome
DecreaseIntracellular calcium
Study
n: not reported in source
PC12 cells exposed to amyloid-beta 25-35
Comparator
Amyloid-beta exposure without Noopept
Outcome group
Receptor activity and mechanism · Nervous system and cognition
Studied dose
10 μM · 72 hours before Аβ 25-35As given in this study.
Source sentences
Summary
Noopept treatment before the amyloid-beta exposure improved PC12 cells viability, reduced the number of early and late apoptotic cells, the levels of intracellular reactive oxygen species and calcium and enhanced the mitochondrial membrane potential.
1 context sentence(design, population, supporting numbers)
Summary · context
The neuroprotective effect of noopept (added to the medium at 10 μM concentration, 72 hours before Аβ 25-35) was studied on Аβ 25-35-induced injury (5 μM for 24 h) in PC12 cells.
Show in the study text Report a problemFND-2509678005
06LabIn vitro (cells)Efficacy outcome
In amyloid-beta-exposed PC12 cells, Noopept pretreatment enhanced mitochondrial membrane potential.
In plain wordsIn amyloid-beta-exposed cells, Noopept pretreatment increased mitochondrial membrane potential compared with amyloid-beta exposure without Noopept.
Peptide
Noopept (omberacetam)
Outcome
IncreaseMitochondrial membrane potential
Study
n: not reported in source
PC12 cells exposed to amyloid-beta 25-35
Comparator
Amyloid-beta exposure without Noopept
Outcome group
Mitochondrial function · Nervous system and cognition
Studied dose
10 μM · 72 hours before Аβ 25-35As given in this study.
Source sentences
Summary
Noopept treatment before the amyloid-beta exposure improved PC12 cells viability, reduced the number of early and late apoptotic cells, the levels of intracellular reactive oxygen species and calcium and enhanced the mitochondrial membrane potential.
1 context sentence(design, population, supporting numbers)
Summary · context
The neuroprotective effect of noopept (added to the medium at 10 μM concentration, 72 hours before Аβ 25-35) was studied on Аβ 25-35-induced injury (5 μM for 24 h) in PC12 cells.
Show in the study text Report a problemFND-2509678006
07LabIn vitro (cells)Efficacy outcome
In amyloid-beta-exposed PC12 cells, Noopept pretreatment attenuated tau hyperphosphorylation at Ser396.
In plain wordsIn amyloid-beta-exposed cells, Noopept pretreatment reduced tau hyperphosphorylation at Ser396 compared with amyloid-beta exposure without Noopept.
Peptide
Noopept (omberacetam)
Outcome
DecreaseTau hyperphosphorylation at Ser396
Study
n: not reported in source
PC12 cells exposed to amyloid-beta 25-35
Comparator
Amyloid-beta exposure without Noopept
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
10 μM · 72 hours before Аβ 25-35As given in this study.
Source sentences
Summary
In addition, pretreatment of PC12 cell with noopept significantly attenuated tau hyperphosphorylation at Ser396 and ameliorated the alterations of neurite outgrowth evoked by Аβ25-35.
1 context sentence(design, population, supporting numbers)
Summary · context
The neuroprotective effect of noopept (added to the medium at 10 μM concentration, 72 hours before Аβ 25-35) was studied on Аβ 25-35-induced injury (5 μM for 24 h) in PC12 cells.
Show in the study text Report a problemFND-2509678007
08LabIn vitro (cells)Efficacy outcome
In amyloid-beta-exposed PC12 cells, Noopept pretreatment ameliorated alterations of neurite outgrowth.
In plain wordsIn amyloid-beta-exposed cells, Noopept pretreatment reduced neurite growth alterations compared with amyloid-beta exposure without Noopept.
Peptide
Noopept (omberacetam)
Outcome
DecreaseNeurite outgrowth alterations
Study
n: not reported in source
PC12 cells exposed to amyloid-beta 25-35
Comparator
Amyloid-beta exposure without Noopept
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
10 μM · 72 hours before Аβ 25-35As given in this study.
Source sentences
Summary
In addition, pretreatment of PC12 cell with noopept significantly attenuated tau hyperphosphorylation at Ser396 and ameliorated the alterations of neurite outgrowth evoked by Аβ25-35.
1 context sentence(design, population, supporting numbers)
Summary · context
The neuroprotective effect of noopept (added to the medium at 10 μM concentration, 72 hours before Аβ 25-35) was studied on Аβ 25-35-induced injury (5 μM for 24 h) in PC12 cells.
Show in the study text Report a problemFND-2509678008

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

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Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
21 Oct 2026, 12:50 UTC
First added · source update from Europe PMC

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