Role of AMPK and PPARγ1 in exercise-induced lipoprotein lipase in skeletal muscle.

Sasaki T, Nakata R, Inoue H, Shimizu M, Inoue J, Sato R

American journal of physiology. Endocrinology and metabolism18 Mar 2014Cited by 31 (Europe PMC)

PMID24644240DOI10.1152/ajpendo.00691.2013PepBankPUB-24644240

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study11

9 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Mechanism
In mice, AICAR increased gastrocnemius PPARγ1 mRNA.
In plain wordsIn mice, AICAR increased PPARγ1 gene expression in muscle.
Peptide
AICAR (acadesine)
Outcome
IncreasePPARγ1 mRNA
Study
mousen: not reported in source
gastrocnemius muscle
Outcome group
Receptor activity and mechanism · Musculoskeletal
Studied dose
Dose not stated · intraperitonealAs given in this study (mouse).
Source sentences
Summary
When mice were intraperitoneally administered an AMP-activated protein kinase (AMPK) activator 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR), the mRNA levels of the aforementioned three genes increased in gastrocnemius muscle.
1 context sentence(design, population, supporting numbers)
Summary · context
Here we show that the mRNA levels of one of peroxisome proliferator-activated receptor (PPAR) family members, PPARγ1, and genes related to energy metabolism, including PPARγ coactivator-1 protein-1α (PGC-1α) and lipoprotein lipase (LPL), increased in the gastrocnemius muscle of habitual exercise-trained mice.
Report a problemFND-2464424001
02AnimalAnimal study (in vivo)Mechanism
In mice, AICAR increased gastrocnemius PGC-1α mRNA.
In plain wordsIn mice, AICAR increased PGC-1α gene expression in muscle.
Peptide
AICAR (acadesine)
Outcome
IncreasePGC-1α mRNA
Study
mousen: not reported in source
gastrocnemius muscle
Outcome group
Receptor activity and mechanism · Musculoskeletal
Studied dose
Dose not stated · intraperitonealAs given in this study (mouse).
Source sentences
Summary
When mice were intraperitoneally administered an AMP-activated protein kinase (AMPK) activator 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR), the mRNA levels of the aforementioned three genes increased in gastrocnemius muscle.
1 context sentence(design, population, supporting numbers)
Summary · context
Here we show that the mRNA levels of one of peroxisome proliferator-activated receptor (PPAR) family members, PPARγ1, and genes related to energy metabolism, including PPARγ coactivator-1 protein-1α (PGC-1α) and lipoprotein lipase (LPL), increased in the gastrocnemius muscle of habitual exercise-trained mice.
Report a problemFND-2464424002
03AnimalAnimal study (in vivo)Mechanism
In mice, AICAR increased gastrocnemius LPL mRNA.
In plain wordsIn mice, AICAR increased lipoprotein lipase gene expression in muscle.
Peptide
AICAR (acadesine)
Outcome
IncreaseLPL mRNA
Study
mousen: not reported in source
gastrocnemius muscle
Outcome group
Receptor activity and mechanism · Musculoskeletal
Studied dose
Dose not stated · intraperitonealAs given in this study (mouse).
Source sentences
Summary
When mice were intraperitoneally administered an AMP-activated protein kinase (AMPK) activator 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR), the mRNA levels of the aforementioned three genes increased in gastrocnemius muscle.
1 context sentence(design, population, supporting numbers)
Summary · context
Here we show that the mRNA levels of one of peroxisome proliferator-activated receptor (PPAR) family members, PPARγ1, and genes related to energy metabolism, including PPARγ coactivator-1 protein-1α (PGC-1α) and lipoprotein lipase (LPL), increased in the gastrocnemius muscle of habitual exercise-trained mice.
Report a problemFND-2464424003
04LabIn vitro (cells)Mechanism
In C2C12 myotubes, AICAR increased PPARγ1 mRNA.
In plain wordsIn cells, AICAR increased PPARγ1 gene expression.
Peptide
AICAR (acadesine)
Outcome
IncreasePPARγ1 mRNA
Study
n: not reported in source
C2C12 differentiated myotubes
Outcome group
Receptor activity and mechanism · Musculoskeletal
Studied dose
Dose not stated
Source sentences
Summary
AICAR treatment to C2C12 differentiated myotubes also increased PPARγ1 mRNA levels, but not PPARα and -δ mRNA levels, concomitant with increased PGC-1α mRNA levels.
Report a problemFND-2464424004
05LabIn vitro (cells)Mechanism
In C2C12 myotubes, AICAR did not increase PPARα mRNA.
In plain wordsIn cells, AICAR did not increase PPARα gene expression.
Peptide
AICAR (acadesine)
Outcome
No significant changePPARα mRNA
Study
n: not reported in source
C2C12 differentiated myotubes
Outcome group
Receptor activity and mechanism · Musculoskeletal
Studied dose
Dose not stated
Source sentences
Summary
AICAR treatment to C2C12 differentiated myotubes also increased PPARγ1 mRNA levels, but not PPARα and -δ mRNA levels, concomitant with increased PGC-1α mRNA levels.
Report a problemFND-2464424005
06LabIn vitro (cells)Mechanism
In C2C12 myotubes, AICAR did not increase PPARδ mRNA.
In plain wordsIn cells, AICAR did not increase PPARδ gene expression.
Peptide
AICAR (acadesine)
Outcome
No significant changePPARδ mRNA
Study
n: not reported in source
C2C12 differentiated myotubes
Outcome group
Receptor activity and mechanism · Musculoskeletal
Studied dose
Dose not stated
Source sentences
Summary
AICAR treatment to C2C12 differentiated myotubes also increased PPARγ1 mRNA levels, but not PPARα and -δ mRNA levels, concomitant with increased PGC-1α mRNA levels.
Report a problemFND-2464424006
07LabIn vitro (cells)Mechanism
In C2C12 myotubes, AICAR increased PGC-1α mRNA.
In plain wordsIn cells, AICAR increased PGC-1α gene expression.
Peptide
AICAR (acadesine)
Outcome
IncreasePGC-1α mRNA
Study
n: not reported in source
C2C12 differentiated myotubes
Outcome group
Receptor activity and mechanism · Musculoskeletal
Studied dose
Dose not stated
Source sentences
Summary
AICAR treatment to C2C12 differentiated myotubes also increased PPARγ1 mRNA levels, but not PPARα and -δ mRNA levels, concomitant with increased PGC-1α mRNA levels.
Report a problemFND-2464424007
08LabIn vitro (cells)Mechanism
In C2C12 cells, AICAR increased PPARγ1 mRNA half-life nearly threefold through AMPK activation.
In plain wordsIn cells, AICAR increased PPARγ1 mRNA half-life nearly threefold.
Peptide
AICAR (acadesine)
Outcome
IncreasePPARγ1 mRNA half-life
Study
n: not reported in source
C2C12 myotubes
Outcome group
Receptor activity and mechanism · Musculoskeletal
Studied dose
Dose not stated
Measurements
PPARγ1 mRNA half-life
4 h
before AICAR-associated increase
PPARγ1 mRNA half-life
12 h
after AICAR-associated increase
Source sentences
Summary
AICAR treatment increased the half-life of PPARγ1 mRNA nearly threefold (4-12 h) by activating AMPK.
Report a problemFND-2464424009
09LabIn vitro (cells)Mechanism
In C2C12 myotubes, AICAR increased LPL gene expression.
In plain wordsIn cells, AICAR increased lipoprotein lipase gene expression.
Peptide
AICAR (acadesine)
Outcome
IncreaseLPL gene expression
Study
n: not reported in source
C2C12 myotubes
Outcome group
Receptor activity and mechanism · Musculoskeletal
Studied dose
Dose not stated
Source sentences
Summary
In contrast, when PPARγ1 expression was suppressed in C2C12 myotubes, LPL mRNA levels were significantly reduced, and the effect of AICAR on increased LPL gene expression was almost completely blocked.
Report a problemFND-2464424011

Studies that did not test the peptide alone2

In this study, AICAR (acadesine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking AICAR (acadesine).

10LabNot a test of AICAR (acadesine) aloneIn vitro (cells)Mechanism
In C2C12 myotubes, compound C blocked the effects of AICAR on gene expression.
In plain wordsIn cells, compound C blocked AICAR's effects on gene expression.
Peptide
AICAR (acadesine)
Outcome
DecreaseBlockade of AICAR effects by compound C
Study
n: not reported in source
C2C12 differentiated myotubes
Outcome group
Receptor activity and mechanism · Musculoskeletal
Source sentences
Summary
An AMPK inhibitor, compound C, blocked these AICAR effects.
1 context sentence(design, population, supporting numbers)
Summary · context
AICAR treatment to C2C12 differentiated myotubes also increased PPARγ1 mRNA levels, but not PPARα and -δ mRNA levels, concomitant with increased PGC-1α mRNA levels.
In this study, AICAR (acadesine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking AICAR (acadesine).
Report a problemFND-2464424008
11LabNot a test of AICAR (acadesine) aloneIn vitro (cells)Mechanism
In C2C12 myotubes, PPARγ1 suppression reduced LPL mRNA and almost completely blocked the AICAR-induced increase in LPL expression.
In plain wordsIn cells, suppressing PPARγ1 reduced LPL expression and almost completely blocked its increase with AICAR.
Peptide
AICAR (acadesine)
Outcome
DecreaseLPL mRNA after PPARγ1 suppression
Study
n: not reported in source
PPARγ1-suppressed C2C12 myotubes
Outcome group
Receptor activity and mechanism · Musculoskeletal
Source sentences
Summary
In contrast, when PPARγ1 expression was suppressed in C2C12 myotubes, LPL mRNA levels were significantly reduced, and the effect of AICAR on increased LPL gene expression was almost completely blocked.
In this study, AICAR (acadesine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking AICAR (acadesine).
Report a problemFND-2464424010

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
21 Oct 2026, 12:50 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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